Wheat with reduced lipoxygenase activity
A series of independent human-induced non-transgenic mutations found at one or more of the Lpx genes of wheat; wheat plants having these mutations in one or more of their Lpx genes; and a method of creating and finding similar and/or additional mutations of Lpx by screening pooled and/or individual wheat plants. The wheat plants disclosed herein exhibit decreased lipoxygenase activity without having the inclusion of foreign nucleic acids in their genomes. Additionally, products produced from the wheat plants disclosed herein display increased oxidative stability and increased shelf life without having the inclusion of foreign nucleic acids in their genomes.
1. A wheat plant comprising a human induced alteration in an Lpx1 gene in a D genome, wherein milled grain from said wheat plant has a property selected from the group consisting of: (a) increased shelf-life; (b) increased oxidative stability; (c) decreased production of Lpx1 protein; (d) decreased lipoxygenase activity; (e) decreased hexanal production; (f) decreased pinellic acid production; (g) decreased decomposition products from fatty acids; and (h) improved sensory characteristics as compared to milled grain from a wild type wheat plant.
2. The wheat plant of claim 1 , wherein the Lpx1 gene is Lpx-D1.
3. The wheat plant of claim 1 , further comprising a human induced alteration in a Lpx1 gene of a B genome.
4. The wheat plant of claim 3 , wherein the Lpx1 gene of the B genome is Lpx-B1.2.
5. The wheat plant of claim 1 , wherein the human induced alteration in the Lpx1 gene of the D genome is in a promoter.
6. Wheat grain from the wheat plant of claim 1 .
7. Flour comprising wheat grain of claim 6 .
8. A wheat seed, plant part or progeny thereof from a wheat plant of claim 1 , wherein said progeny comprises the alteration in the Lpx1 gene.
9. A wheat plant comprising a human induced alteration in an Lpx1 gene in each of a B and a D genome, wherein milled grain from said wheat plant has a property selected from the group consisting of: (a) increased shelf-life; (b) increased oxidative stability; (c) decreased production of Lpx1 protein; (d) decreased lipoxygenase activity; (e) decreased hexanal production; (f) decreased pinellic acid production; (g) decreased decomposition products from fatty acids; and (h) improved sensory characteristics as compared to milled grain from a wild type wheat plant.
10. The wheat plant of claim 9 , wherein the Lpx1 gene in the D genome is Lpx-D1.
11. The wheat plant of claim 9 , wherein the Lpx1 gene in the B genome is Lpx-B1.2.
12. Wheat grain from the wheat plant of claim 9 .
13. Flour comprising wheat grain of claim 12 .
14. A wheat seed, plant part or progeny thereof from a wheat plant of claim 9 , wherein said progeny comprises the alteration in the Lpx1 gene.
15. Wheat grain comprising an embryo and a reduced activity of lipoxygenase, wherein the embryo comprises a human induced alteration in an Lpx1 gene in a D genome, and further wherein the lipoxygenase activity is reduced by at least 9% relative to the lipoxygenase activity in a wild type parental wheat grain.
16. The wheat grain of claim 15 , wherein the human induced alteration in the Lpx1 gene of the D genome is in a promoter.
17. The wheat grain of claim 15 , wherein the embryo further comprises a human induced alteration in an Lpx1 gene in a B genome.
18. The wheat grain of claim 15 , wherein the lipoxygenase activity is reduced by at least 20% relative to the lipoxygenase activity in the wild type parental wheat grain.
19. The wheat grain of claim 15 , wherein the lipoxygenase activity is reduced by at least 60% relative to the lipoxygenase activity in the wild type parental wheat grain.
20. Flour comprising wheat grain of claim 15 .