Granted Patent
B2
US 12,404,493 · App. 15/994,302 · Granted Sep 2, 2025
Methods for the re-derivation of diverse pluripotent stem cell-derived brown fat cells
Assignee:
AgeX Therapeutics, Inc.
View Patent ↗
Loading inventors, assignments & file history…
Abstract
Aspects of the present invention include methods and compositions related to the production and use of pluripotent stem cell-derived clonal embryonic progenitor cell types useful in the generation of cellular components of brown adipocyte tissue for research and therapy relating to applications in obesity, diabetes, and cardiovascular disease.
Claims (27)
1. A method of producing brown adipocytes comprising:
a. differentiating a human pluripotent stem cell in a neural differentiation medium consisting of DMEM/F12, B27 supplement, and basic Fibroblast Growth Factor (bFGF), thereby forming an embryoid body;
b. producing a clonal embryonic progenitor cell line from the embryoid body, wherein said clonal embryonic progenitor cell line expresses DIO3, SLC1A3, SBSN, DLK1 and ZIC2, but does not express COX7A1, DLX5, HOXA5, IL13RA2, CRABP1, NEFM, PRG4, and RBP1;
c. differentiating the clonal embryonic progenitor cell line in the presence of a PPARgamma (PPARy) agonist, a thyroid hormone, and a TGFβ family member polypeptide, thereby producing a differentiated progeny from the clonal embryonic progenitor cell line;
d. screening the differentiated progeny from the clonal embryonic progenitor cell line for the expression of a brown adipocyte marker comprising UCP1; and
e. isolating UCP1 expressing brown adipocytes.
2. The method of claim 1 , wherein the PPARgamma agonist is rosiglitazone.
3. The method of claim 1 , wherein the UCP1 expressing brown adipocytes are further characterized by the expression of one or more of ADIPOQ, C19orf80, FABP4 or CD36.
4. A method of producing brown adipocytes comprising:
a. differentiating a human pluripotent stem cell in a neural differentiation medium consisting of DMEM/F12, B27 supplement, and basic Fibroblast Growth Factor (bFGF), thereby forming an embryoid body;
b. producing a clonal embryonic progenitor cell line from the embryoid body, wherein said clonal embryonic progenitor cell line expresses HOXA2, HOXB2, NEFM, RBP1 and DLK1, but does not express COX7A1, IL13RA2, ZIC2, DLX5, PRG4, CRABP1 and SBSN;
c. differentiating the clonal embryonic progenitor cell line in the presence of a PPARgamma (PPARy) agonist, a thyroid hormone, and a TGFβ family member polypeptide, thereby producing a differentiated progeny from the clonal embryonic progenitor cell line;
d. screening the differentiated progeny from the clonal embryonic progenitor cell line for the expression of a brown adipocyte marker comprising UCP1; and
e. isolating UCP1 expressing brown adipocytes.
5. The method of claim 4 , wherein the PPARgamma agonist is rosiglitazone.
6. The method of claim 4 , wherein the UCP1 expressing brown adipocytes are further characterized by the expression of one or more of ADIPOQ, C19orf80, FABP4 or CD36.
7. A method of producing brown adipocytes comprising:
a. differentiating a human pluripotent stem cell in a neural differentiation medium consisting of DMEM/F12, B27 supplement, and basic Fibroblast Growth Factor (bFGF), thereby forming an embryoid body;
b. producing a clonal embryonic progenitor cell line from the embryoid body, wherein said clonal embryonic progenitor cell line expresses RBP1, ZIC2 and HOXA2, but does not express COX7A1, HOXB2, HOXA5, NEFM, PRG4, DLX5, IL13RA2, CRABP1, and SBSN;
c. differentiating the clonal embryonic progenitor cell line in the presence of a PPARgamma (PPARy) agonist, a thyroid hormone, and a TGFβ family member polypeptide, thereby producing a differentiated progeny from the clonal embryonic progenitor cell line;
d. screening the differentiated progeny from the clonal embryonic progenitor cell line for the expression of a brown adipocyte marker comprising UCP1; and
e. isolating UCP1 expressing brown adipocytes.
8. The method of claim 7 , wherein the PPARgamma agonist is rosiglitazone.
9. The method of claim 7 , wherein the UCP1 expressing brown adipocytes are further characterized by the expression of one or more of ADIPOQ, C19orf80, FABP4 or CD36.
10. The method of claim 1 , wherein the TGFβ family member polypeptide is BMP4.
11. The method of claim 4 , wherein the TGFβ family member polypeptide is BMP4.
12. The method of claim 7 , wherein the TGFβ family member polypeptide is BMP4.
Assignments (4)
SECURITY INTEREST
Recorded Apr 8, 2024
From: UNIVERXOME BIOENGINEERING, INC.
To: JUVENESCENCE LIMITED
Reel/Frame 067037/0625 →
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded Oct 7, 2020
From: LINEAGE CELL THERAPEUTICS, INC.
To: AGEX THERAPEUTICS, INC.
Reel/Frame 054001/0950 →
CHANGE OF NAME
Recorded Oct 7, 2020
From: BIOTIME, INC.
To: LINEAGE CELL THERAPEUTICS, INC.
Reel/Frame 054005/0749 →
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded Jun 11, 2019
From: WEST, MICHAEL D.; STERNBERG, HAL
To: BIOTIME, INC.
Reel/Frame 049433/0427 →
Continuity (3)
Continuation
PCTUS2016065366
· Dec 7, 2016
Provisional Application
62264311
· Dec 7, 2015
References Cited (400)
US 5030015A
· Baker et al.
· 1991
[cited by applicant]
US 5489508A
· West et al.
· 1996
[cited by applicant]
US 5639613A
· Shay et al.
· 1997
[cited by applicant]
US 5686306A
· West et al.
· 1997
[cited by applicant]
US 5843780A
· Thomson
· 1998
[cited by applicant]
US 5922601A
· Baetscher et al.
· 1999
[cited by applicant]
US 6200806B1
· Thomson
· 2001
[cited by applicant]
US 6248934B1
· Tessier-Lavigne et al.
· 2001
[cited by applicant]
US 6280718B1
· Kaufman et al.
· 2001
[cited by applicant]
US 6465493B1
· Burgess et al.
· 2002
[cited by applicant]
US 6602711B1
· Thomson et al.
· 2003
[cited by applicant]
US 6667176B1
· Funk et al.
· 2003
[cited by applicant]
US 6685911B1
· Zitvogel et al.
· 2004
[cited by applicant]
US 6887706B2
· Zhang et al.
· 2005
[cited by applicant]
US 7176023B2
· Kaufman et al.
· 2007
[cited by applicant]
US 7217569B2
· Thomson
· 2007
[cited by applicant]
US 7253334B2
· Collas et al.
· 2007
[cited by applicant]
US 7417041B2
· Blumberg et al.
· 2008
[cited by applicant]
US 7582479B2
· Thomson
· 2009
[cited by applicant]
US 7585622B1
· Cech et al.
· 2009
[cited by applicant]
US 7625573B2
· Zitvogel et al.
· 2009
[cited by applicant]
US 7736895B2
· Collas et al.
· 2010
[cited by applicant]
US 7928069B2
· Prestwich et al.
· 2011
[cited by applicant]
US 7951591B2
· Robl et al.
· 2011
[cited by applicant]
US 7981871B2
· Prestwich et al.
· 2011
[cited by applicant]
US 8021847B2
· Pietrzkowski
· 2011
[cited by applicant]
US 8058065B2
· Yamanaka et al.
· 2011
[cited by applicant]
US 8090542B2
· Khvorova et al.
· 2012
[cited by applicant]
US 8236774B2
· Cech et al.
· 2012
[cited by applicant]
US 8247384B2
· Green et al.
· 2012
[cited by applicant]
US 8324184B2
· Prestwich et al.
· 2012
[cited by applicant]
US 8476017B2
· Pietrzkowski
· 2013
[cited by applicant]
US 8685386B2
· West et al.
· 2014
[cited by applicant]
US 9175263B2
· Larocca et al.
· 2015
[cited by applicant]
US 20010039316A1
· Campbell et al.
· 2001
[cited by applicant]
US 20020001842A1
· Chapman
· 2002
[cited by applicant]
US 20020051762A1
· Rafii et al.
· 2002
[cited by applicant]
US 20020069484A1
· Creel
· 2002
[cited by applicant]
US 20020142397A1
· Collas et al.
· 2002
[cited by applicant]
US 20030046722A1
· Collas et al.
· 2003
[cited by applicant]
US 20030129745A1
· Robl et al.
· 2003
[cited by applicant]
US 20030149277A1
· Gaster et al.
· 2003
[cited by applicant]
US 20030161817A1
· Young et al.
· 2003
[cited by applicant]
US 20030166633A1
· Gaster et al.
· 2003
[cited by applicant]
US 20030180268A1
· Atala
· 2003
[cited by applicant]
US 20030232430A1
· Cibelli et al.
· 2003
[cited by applicant]
US 20040001807A1
· Edelberg et al.
· 2004
[cited by applicant]
US 20040039198A1
· Bender et al.
· 2004
[cited by applicant]
US 20040063745A1
· Gellibert et al.
· 2004
[cited by applicant]
US 20040152738A1
· Gaster et al.
· 2004
[cited by applicant]
US 20040199935A1
· Chapman
· 2004
[cited by applicant]
US 20040219563A1
· West et al.
· 2004
[cited by applicant]
US 20040220230A1
· Gaster et al.
· 2004
[cited by applicant]
US 20040228847A1
· Goldschmidt-Clermont et al.
· 2004
[cited by applicant]
US 20040247567A1
· Gurtner
· 2004
[cited by applicant]
US 20040266842A1
· Gaster et al.
· 2004
[cited by applicant]
US 20050014258A1
· Collas et al.
· 2005
[cited by applicant]
US 20050014938A1
· Gaster et al.
· 2005
[cited by applicant]
US 20050165011A1
· Gellibert et al.
· 2005
[cited by applicant]
US 20050250727A1
· Tasken et al.
· 2005
[cited by applicant]
US 20060003446A1
· Keller et al.
· 2006
[cited by applicant]
US 20060171930A1
· Seyda et al.
· 2006
[cited by applicant]
US 20060212952A1
· Collas et al.
· 2006
[cited by applicant]
US 20070072901A1
· Washio
· 2007
[cited by applicant]
US 20070116684A1
· Atala et al.
· 2007
[cited by applicant]
US 20070154428A1
· Sato et al.
· 2007
[cited by applicant]
US 20070154474A1
· Soppet et al.
· 2007
[cited by applicant]
US 20070155013A1
· Akaike et al.
· 2007
[cited by applicant]
US 20080070303A1
· West et al.
· 2008
[cited by applicant]
US 20080233610A1
· Thomson
· 2008
[cited by applicant]
US 20090047263A1
· Yamanaka
· 2009
[cited by applicant]
US 20090104159A1
· Prosper et al.
· 2009
[cited by applicant]
US 20090148423A1
· Sumitran-Holgersson
· 2009
[cited by applicant]
US 20090220450A1
· Green et al.
· 2009
[cited by applicant]
US 20100003757A1
· Mack
· 2010
[cited by applicant]
US 20100055678A1
· Jaatinen et al.
· 2010
[cited by applicant]
US 20100111914A1
· Zhang et al.
· 2010
[cited by applicant]
US 20100158872A1
· Keller et al.
· 2010
[cited by applicant]
US 20100166713A1
· Dalton et al.
· 2010
[cited by applicant]
US 20100167404A1
· West et al.
· 2010
[cited by applicant]
US 20100183620A1
· Bhawe et al.
· 2010
[cited by applicant]
US 20100184033A1
· West et al.
· 2010
[cited by applicant]
US 20100203021A1
· Goumans et al.
· 2010
[cited by applicant]
US 20100255580A1
· Rezania
· 2010
[cited by applicant]
US 20100267135A1
· Sakurada et al.
· 2010
[cited by applicant]
US 20100330063A1
· Weinstein
· 2010
[cited by applicant]
US 20110038791A1
· Ford et al.
· 2011
[cited by applicant]
US 20110104133A1
· Tseng
· 2011
[cited by examiner]
US 20110143441A1
· West et al.
· 2011
[cited by applicant]
US 20110218143A1
· Kaushal et al.
· 2011
[cited by applicant]
US 20110223669A1
· Yamanaka et al.
· 2011
[cited by applicant]
US 20110312881A1
· Silverman et al.
· 2011
[cited by applicant]
US 20120060232A1
· Stan
· 2012
[cited by applicant]
US 20120093885A1
· Sahoo et al.
· 2012
[cited by applicant]
US 20120148546A1
· Dar-Oaknin et al.
· 2012
[cited by applicant]
US 20120171171A1
· West et al.
· 2012
[cited by applicant]
US 20120295347A1
· Kessler
· 2012
[cited by applicant]
US 20120301443A1
· Raffi et al.
· 2012
[cited by applicant]
US 20130015622A1
· Hata
· 2013
[cited by applicant]
US 20130115609A1
· Ho
· 2013
[cited by applicant]
US 20130115673A1
· West et al.
· 2013
[cited by applicant]
US 20130196865A1
· Hochedlinger et al.
· 2013
[cited by applicant]
US 20130202564A1
· Han et al.
· 2013
[cited by applicant]
US 20140010801A1
· Niedernhofer et al.
· 2014
[cited by applicant]
US 20140178988A1
· West et al.
· 2014
[cited by applicant]
US 20140349396A1
· West et al.
· 2014
[cited by applicant]
US 20150004144A1
· Cowan
· 2015
[cited by examiner]
US 20150017718A1
· Nakatsuji et al.
· 2015
[cited by applicant]
US 20150079110A1
· Burns et al.
· 2015
[cited by applicant]
US 20150275177A1
· West et al.
· 2015
[cited by applicant]
US 20160002597A1
· Sinden et al.
· 2016
[cited by applicant]
US 20160193252A1
· Hicks et al.
· 2016
[cited by applicant]
US 20160369237A1
· West et al.
· 2016
[cited by applicant]
US 20170108503A1
· Klass et al.
· 2017
[cited by applicant]
US 20180170982A1
· West et al.
· 2018
[cited by applicant]
US 20180273906A1
· Ashraf
· 2018
[cited by applicant]
US 20190151372A1
· Larocca et al.
· 2019
[cited by applicant]
US 20190175691A1
· West et al.
· 2019
[cited by applicant]
US 20190218511A1
· Larocca et al.
· 2019
[cited by applicant]
US 20220088137A1
· West et al.
· 2022
[cited by applicant]
US 20220088138A1
· West et al.
· 2022
[cited by applicant]
US 20220098554A1
· Yang et al.
· 2022
[cited by applicant]
US 20220160833A1
· West et al.
· 2022
[cited by applicant]
US 20220401494A1
· Larocca et al.
· 2022
[cited by applicant]
US 20220403341A1
· Larocca et al.
· 2022
[cited by applicant]
US 20230093399A1
· West et al.
· 2023
[cited by applicant]
CN 102105789A
· 2011
[cited by applicant]
CN 104114693A
· 2014
[cited by applicant]
CN 104350146A
· 2015
[cited by applicant]
CN 105435244A
· 2016
[cited by applicant]
EP 1001806A1
· 2000
[cited by applicant]
EP 1523990A1
· 2005
[cited by applicant]
EP 1860180A1
· 2007
[cited by applicant]
EP 2254586A1
· 2010
[cited by applicant]
EP 2496711A2
· 2012
[cited by applicant]
EP 3387112A1
· 2018
[cited by applicant]
EP 3463391A1
· 2019
[cited by applicant]
JP 2013151437A
· 2013
[cited by applicant]
JP 2016518393A
· 2016
[cited by applicant]
JP 2019517538A
· 2019
[cited by applicant]
WO WO199830679A1
· 1998
[cited by applicant]
WO WO1999003499A1
· 1999
[cited by applicant]
WO WO199920741A1
· 1999
[cited by applicant]
WO WO200100650A1
· 2001
[cited by applicant]
WO WO200130978A1
· 2001
[cited by applicant]
WO WO200151616A2
· 2001
[cited by applicant]
WO WO2003046141A2
· 2003
[cited by applicant]
WO WO2003074654A2
· 2003
[cited by applicant]
WO WO2003076603A2
· 2003
[cited by applicant]
WO WO2005068610A1
· 2005
[cited by applicant]
WO WO2005121369A2
· 2005
[cited by applicant]
WO 2006054262A2
· 2006
[cited by applicant]
WO WO2006130504A2
· 2006
[cited by applicant]
WO WO2007019398A1
· 2007
[cited by applicant]
WO WO2007047894A2
· 2007
[cited by applicant]
WO 2007062198A1
· 2007
[cited by applicant]
WO WO2007058671A1
· 2007
[cited by applicant]
WO WO2008089448A2
· 2008
[cited by applicant]
WO WO2008148938A1
· 2008
[cited by applicant]
WO WO2009052211A1
· 2009
[cited by applicant]
WO WO2009105044A1
· 2009
[cited by applicant]
WO WO2010021993A1
· 2010
[cited by applicant]
WO WO2011053257A2
· 2011
[cited by applicant]
WO WO2011150105A2
· 2011
[cited by applicant]
WO WO2012020308A2
· 2012
[cited by applicant]
WO 2012065065A1
· 2012
[cited by applicant]
WO WO2012125471A1
· 2012
[cited by applicant]
WO 2012147470A1
· 2012
[cited by applicant]
WO WO2013003595A1
· 2013
[cited by applicant]
WO WO2013014691A1
· 2013
[cited by applicant]
WO WO2013036969A1
· 2013
[cited by applicant]
WO 2013082268A1
· 2013
[cited by applicant]
WO 2013084000A2
· 2013
[cited by applicant]
WO WO2013150303A1
· 2013
[cited by applicant]
WO WO2013172793A1
· 2013
[cited by applicant]
WO WO2014013258A1
· 2014
[cited by applicant]
WO WO2014022852A1
· 2014
[cited by applicant]
WO WO2014028493A2
· 2014
[cited by applicant]
WO WO2014091373A1
· 2014
[cited by applicant]
WO WO2014125276A1
· 2014
[cited by applicant]
WO WO2014125277A1
· 2014
[cited by applicant]
WO WO2014197421A1
· 2014
[cited by applicant]
WO WO2015052526A1
· 2015
[cited by applicant]
WO WO2015052527A1
· 2015
[cited by applicant]
WO 2017100313A1
· 2017
[cited by applicant]
WO 2017214342A1
· 2017
[cited by applicant]
WO 2019178296A1
· 2019
[cited by applicant]
WO 2019209892A1
· 2019
[cited by applicant]
WO 2020069373A1
· 2020
[cited by applicant]
Ohno et al. PPAR agonists induce a white-to-brown fat conversion through stabilization of PRDM16 protein. Cell Metab. Mar. 7, 2012; 15(3): 395-404 (Year: 2012).
[cited by examiner]
Nedergaard et al. PPAR in the control of brown adipocyte differentiation. Biochimica et Biophysica Acta 1740 (2005) 293-304. (Year: 2005).
[cited by examiner]
Abad et al., Reprogramming in vivo produces teratomas and iPS cells with totipotency features. Nature. 2013;502(7471):340-345.
[cited by applicant]
Bodnar et al., Extension of life-span by introduction of telomerase into normal human cells. Science. 1998;279(5349):349-352.
[cited by applicant]
Chuang et al., The fission yeast homologue of Orc4p binds to replication origin DNA via multiple AT-hooks. Proc Natl Acad Sci U S A. 1999;96(6):2656-2661.
[cited by applicant]
Crisan et al., A perivascular origin for mesenchymal stem cells in multiple human organs. Cell Stem Cell. Sep. 11, 2008;3(3):301-13.
[cited by applicant]
Cruz et al., Extracellular Vesicles: Decoding a New Language for Cellular Communication in Early Embryonic Development. Front Cell Dev Biol. Aug. 28, 2018;6:94.
[cited by applicant]
French et al., What is a Conservative Substitution? J Mol Evol. 1983;19:171-175.
[cited by applicant]
Greenwood-Goodwin et al., A novel lineage restricted, pericyte-like cell line isolated from human embryonic stem cells. Sci Rep. Apr. 25, 2016;6:24403, 10 pages.
[cited by applicant]
Horvath, DNA methylation age of human tissues and cell types. Genome Biol. 2013;14(10):R115, 20 pages.
[cited by applicant]
Kaczkowski et al., Transcriptome Analysis of Recurrently Deregulated Genes across Multiple Cancers Identifies New Pan-Cancer Biomarkers. Cancer Res. 2016;76(2):216-226.
[cited by applicant]
Kashyap et al., Regulation of stem cell pluripotency and differentiation involves a mutual regulatory circuit of the Nanog, OCT4, and SOX2 pluripotency transcription factors with polycomb repressive complexes and stem c…
[cited by applicant]
Kim et al., Specific association of human telomerase activity with immortal cells and cancer. Science. 1994;266(5193):2011-2015.
[cited by applicant]
Lanza et al., Extension of cell life-span and telomere length in animals cloned from senescent somatic cells. Science. 2000;288(5466):665-669.
[cited by applicant]
Lu et al., Reversal of ageing-and injury-induced vision loss by Tet-dependent epigenetic reprogramming. bioRxiv, retrieved nline at: https://www.biorxiv.org/content/10.1101/710210v1.full.pdf. 51 pages, Jul. 31, 2019.
[cited by applicant]
Marion et al., Common Telomere Changes during In Vivo Reprogramming and Early Stages of Tumorigenesis. Stem Cell Reports. 2017;8(2):460-475.
[cited by applicant]
Ocampo et al., In Vivo Amelioration of Age-Associated Hallmarks by Partial Reprogramming. Cell. 2016; 167(7):1719-1733.e12.
[cited by applicant]
Olova et al., Partial reprogramming induces a steady decline in epigenetic age before loss of somatic identity. Aging Cell. 2019;18(1):e12877, 7 pages.
[cited by applicant]
Roth et al., Telomerase levels control the lifespan of human T lymphocytes. Blood. 2003;102(3):849-857.
[cited by applicant]
Shyh-Chang et al., Lin28 enhances tissue repair by reprogramming cellular metabolism. Cell. 2013;155(4):778-792.
[cited by applicant]
Vaziri et al., Spontaneous reversal of the developmental aging of normal human cells following transcriptional reprogramming. Regen Med. 2010;5(3):345-363.
[cited by applicant]
West et al., Use of deep neural network ensembles to identify embryonic-fetal transition markers: repression of COX7A1 in embryonic and cancer cells. Oncotarget. Dec. 28, 2017;9(8):7796-7811.
[cited by applicant]
Yuan et al., Exosomes Derived From Pericytes Improve Microcirculation and Protect Blood-Spinal Cord Barrier After Spinal Cord Injury in Mice. Front Neurosci. 2019;13:319, 14 pages.
[cited by applicant]
Ahfeldt et al., Programming human pluripotent stem cells into white and brown adipocytes. Nat Cell Biol. Jan. 15, 2012;14(2):209-19.
[cited by applicant]
Amieux et al., Cyclic nucleotides converge on brown adipose tissue differentiation. Sci Signal. Jan. 12, 2010;3(104):pe2. 3 pages.
[cited by applicant]
Ancey et al., Secretion of IL-6, IL-11 and LIF by human cardiomyocytes in primary culture. Cytokine. May 21, 2002;18(4):199-205.
[cited by applicant]
Armulik et al., Pericytes regulate the blood-brain barrier. Nature. Nov. 25, 2010;468(7323):557-61.
[cited by applicant]
Armulik et al., Pericytes: developmental, physiological, and pathological perspectives, problems, and promises. Dev Cell. Aug. 16, 2011;21(2):193-215.
[cited by applicant]
Bai et al., BMP4 regulates vascular progenitor development in human embryonic stem cells through a Smad-dependent pathway. J Cell Biochem. Feb. 1, 2010;109(2):363-74.
[cited by applicant]
Beranger et al., In vitro brown and “brite”/“beige” adipogenesis: human cellular models and molecular aspects. Biochim Biophys Acta. May 2013;1831(5):905-14.
[cited by applicant]
Bergers et al., The role of pericytes in blood-vessel formation and maintenance. Neuro Oncol. Oct. 2005;7(4):452-64.
[cited by applicant]
Bian et al., Extracellular vesicles derived from human bone marrow mesenchymal stem cells promote angiogenesis in a rat myocardial infarction model. J Mol Med (Berl). Apr. 2014;92(4):387-97.
[cited by applicant]
Bignone et al., Identification of human embryonic progenitor cell targeting peptides using phage display. PLoS One. 2013;8(3):e58200. 12 pages.
[cited by applicant]
Birbrair et al., Role of pericytes in skeletal muscle regeneration and fat accumulation. Stem Cells Dev. Aug. 15, 2013;22(16):2298-314.
[cited by applicant]
Birbrair et al., Type-2 pericytes participate in normal and tumoral angiogenesis. Am J Physiol Cell Physiol. Jul. 1, 2014;307(1):C25-38.
[cited by applicant]
Blanpain et al., Stem cells assessed. Nat Rev Mol Cell Biol. Jun. 8, 2012;13(7):471-6.
[cited by applicant]
Blocki et al., Not all MSCs can act as pericytes: functional in vitro assays to distinguish pericytes from other mesenchymal stem cells in angiogenesis. Stem Cells Dev. Sep. 1, 2013;22(17):2347-55.
[cited by applicant]
Bloom et al., Disodium (R,R)-5-[2-[[2-(3-chlorophenyl)-2-hydroxyethyl]-amino] propyl]-1,3-benzodioxole-2,2-dicarboxylate (CL 316,243). A potent beta-adrenergic agonist virtually specific for beta 3 receptors. A promisin…
[cited by applicant]
Blum et al., The tumorigenicity of diploid and aneuploid human pluripotent stem cells. Cell Cycle. Dec. 2009;8(23):3822-30.
[cited by applicant]
Bogos et al., VEGFR-3-positive circulating lymphatic/vascular endothelial progenitor cell level is associated with poor prognosis in human small cell lung cancer. Clin Cancer Res. Mar. 1, 2009;15(5):1741-6.
[cited by applicant]
Bongso et al., Improved quality of human embryos when co-cultured with human ampullary cells. Hum Reprod. Aug. 1989;4(6):706-13.
[cited by applicant]
Bongso et al., Isolation and culture of inner cell mass cells from human blastocysts. Hum Reprod. Nov. 1994;9(11):2110-7.
[cited by applicant]
Bruno et al., Mesenchymal stem cell-derived microvesicles protect against acute tubular injury. J Am Soc Nephrol. May 2009;20(5):1053-67.
[cited by applicant]
Camussi et al., Exosomes/microvesicles as a mechanism of cell-to-cell communication. Kidney Int. Nov. 2010;78(9):838-48.
[cited by applicant]
Cannon et al., Brown adipose tissue: function and physiological significance. Physiol Rev. Jan. 2004;84(1):277-359.
[cited by applicant]
Cannon et al., Cultures of adipose precursor cells from brown adipose tissue and of clonal brown-adipocyte-like cell lines. Methods Mol Biol. 2001;155:213-24.
[cited by applicant]
Carmeliet, Angiogenesis in life, disease and medicine. Nature. Dec. 15, 2005;438(7070):932-6.
[cited by applicant]
Cheema et al., Regulation and guidance of cell behavior for tissue regeneration via the siRNA mechanism. Wound Repair Regen. May-Jun. 2007;15(3):286-95.
[cited by applicant]
Chen et al., A model of focal ischemic stroke in the rat: reproducible extensive cortical infarction. Stroke. Jul.-Aug. 1986;17(4):738-43.
[cited by applicant]
Chen et al., Enabling a robust scalable manufacturing process for therapeutic exosomes through oncogenic immortalization of human ESC-derived MSCs. J Transl Med. Apr. 25, 2011;9:47. 10 pages.
[cited by applicant]
Chen et al., Human myocardial pericytes: multipotent mesodermal precursors exhibiting cardiac specificity. Stem Cells. Feb. 2015;33(2):557-73.
[cited by applicant]
Chen et al., Human pericytes for ischemic heart repair. Stem Cells. Feb. 2013;31(2):305-16.
[cited by applicant]
Chung et al., Human embryonic stem cell lines generated without embryo destruction. Cell Stem Cell. Feb. 7, 2008;2(2):113-7.
[cited by applicant]
Cibelli et al., Transgenic bovine chimeric offspring produced from somatic cell-derived stem-like cells. Nat Biotechnol. Jul. 1998;16(7):642-6.
[cited by applicant]
Climent et al., TGFbeta Triggers miR-143/145 Transfer From Smooth Muscle Cells to Endothelial Cells, Thereby Modulating Vessel Stabilization. Circ Res. May 22, 2015;116(11):1753-64.
[cited by applicant]
Cohen et al., Turning straw into gold: directing cell fate for regenerative medicine. Nat Rev Genet. Apr. 2011;12(4):243-52.
[cited by applicant]
Conley et al., BMPs regulate differentiation of a putative visceral endoderm layer within human embryonic stem-cell-derived embryoid bodies. Biochem Cell Biol. Feb. 2007;85(1):121-32.
[cited by applicant]
Cooper et al., Modulation of PGC-1 coactivator pathways in brown fat differentiation through LRP130. J Biol Chem. Nov. 14, 2008;283(46):31960-7.
[cited by applicant]
Corselli et al., Identification of perivascular mesenchymal stromal/stem cells by flow cytometry. Cytometry A. Aug. 2013;83(8):714-20.
[cited by applicant]
Costa et al., The hESC line Envy expresses high levels of GFP in all differentiated progeny. Nat Methods. Apr. 2005;2(4):259-60.
[cited by applicant]
Costa et al., The hESC line Envy expresses high levels of GFP in all differentiated progeny. Nat Methods. Apr. 2005;2(4):259-60. Advance online publication.
[cited by applicant]
Crook et al., The generation of six clinical-grade human embryonic stem cell lines. Cell Stem Cell. Nov. 2007;1(5):490-4.
[cited by applicant]
Dai et al., MicroRNA-223-3p inhibits the angiogenesis of ischemic cardiac microvascular endothelial cells via affecting RPS6KB1/hif-1a signal pathway. PLoS One. Oct. 14, 2014;9(10):e108468. 14 pages.
[cited by applicant]
Daneman et al., Pericytes are required for blood-brain barrier integrity during embryogenesis. Nature. Nov. 25, 2010;468(7323):562-6.
[cited by applicant]
Dar et al., Multipotent vasculogenic pericytes from human pluripotent stem cells promote recovery of murine ischemic limb. Circulation. Jan. 3, 2012;125(1):87-99.
[cited by applicant]
De Souza Batista et al., Omentin plasma levels and gene expression are decreased in obesity. Diabetes. Jun. 2007;56(6):1655-61.
[cited by applicant]
Dechesne et al., Stem Cells from Human Adipose Tissue: A New Tool for Pharmacological Studies and for Clinical Applications. Adipose Stem Cells and Regenerative Medicine. Y.-G. Illouz (Ed.), Springer-Verlag Berlin Heide…
[cited by applicant]
Deregibus et al., Endothelial progenitor cell derived microvesicles activate an angiogenic program in endothelial cells by a horizontal transfer of mRNA. Blood. Oct. 1, 2007;110(7):2440-8.
[cited by applicant]
Desandro et al., Associations and interactions between bare lymphocyte syndrome factors. Mol Cell Biol. Sep. 2000;20(17):6587-99.
[cited by applicant]
Dore-Duffy et al., Morphology and properties of pericytes. Methods Mol Biol. 2011;686:49-68.
[cited by applicant]
Dubois et al., SIRPA is a specific cell-surface marker for isolating cardiomyocytes derived from human pluripotent stem cells. Nat Biotechnol. Oct. 23, 2011;29(11):1011-8.
[cited by applicant]
Durick et al., Hunting with traps: genome-wide strategies for gene discovery and functional analysis. Genome Res. Nov. 1999;9(11):1019-25.
[cited by applicant]
Díez et al., Endothelial progenitor cells undergo an endothelial-to-mesenchymal transition-like process mediated by TGFbetaRI. Cardiovasc Res. Dec. 1, 2010;88(3):502-11.
[cited by applicant]
Elabd et al., Human multipotent adipose-derived stem cells differentiate into functional brown adipocytes. Stem Cells. Nov. 2009;27(11):2753-60.
[cited by applicant]
Elali et al., The role of pericytes in neurovascular unit remodeling in brain disorders. Int J Mol Sci. Apr. 16, 2014;15(4):6453-74.
[cited by applicant]
Espandar et al., Adipose-derived stem cells on hyaluronic acid-derived scaffold: a new horizon in bioengineered cornea. Arch Ophthalmol. Feb. 2012;130(2):202-8.
[cited by applicant]
Fedorenko et al., Mechanism of fatty-acid-dependent UCP1 uncoupling in brown fat mitochondria. Cell. Oct. 12, 2012;151(2):400-13.
[cited by applicant]
Ferrari et al., Transforming growth factor-beta 1 (TGF-beta1) induces angiogenesis through vascular endothelial growth factor (VEGF)-mediated apoptosis. J Cell Physiol. May 2009;219(2):449-58.
[cited by applicant]
Fish et al., miR-126 regulates angiogenic signaling and vascular integrity. Dev Cell. Aug. 2008;15(2):272-84.
[cited by applicant]
Follenzi et al., Gene transfer by lentiviral vectors is limited by nuclear translocation and rescued by HIV-1 pol sequences. Nat Genet. Jun. 2000;25(2):217-22.
[cited by applicant]
Forte et al., MicroRNA-mediated transformation by the Kaposi's sarcoma-associated herpesvirus Kaposin locus. J Virol. Feb. 2015;89(4):2333-41.
[cited by applicant]
Francavilla et al., Transient GFER knockdown in vivo impairs liver regeneration after partial hepatectomy. Int J Biochem Cell Biol. Aug. 2014;53:343-51.
[cited by applicant]
Fu et al., Endothelial cell O-glycan deficiency causes blood/lymphatic misconnections and consequent fatty liver disease in mice. J Clin Invest. Nov. 2008;118(11):3725-37.
[cited by applicant]
Funk et al., Evaluating the genomic and sequence integrity of human ES cell lines; comparison to normal genomes. Stem Cell Res. Mar. 2012;8(2):154-64.
[cited by applicant]
Gao et al., Endothelial progenitor cells control the angiogenic switch in mouse lung metastasis. Science. Jan. 11, 2008;319(5860):195-8.
[cited by applicant]
Garbuzova-Davis et al., Blood-CNS Barrier Impairment in ALS patients versus an animal model. Front Cell Neurosci. Feb. 3, 2014;8:21. 10 pages.
[cited by applicant]
Garcia et al., Glucose Starvation in Cardiomyocytes Enhances Exosome Secretion and Promotes Angiogenesis in Endothelial Cells. PLoS One. Sep. 22, 2015;10(9):e0138849. 23 pages.
[cited by applicant]
Gardner et al., Culture and transfer of human blastocysts increases implantation rates and reduces the need for multiple embryo transfers. Fertil Steril. Jan. 1998;69(1):84-8.
[cited by applicant]
Gehling et al., In vitro differentiation of endothelial cells from AC133-positive progenitor cells. Blood. May 15, 2000,95(10):3106-12.
[cited by applicant]
George et al., Isolation of human platelet membrane microparticles from plasma and serum. Blood. Oct. 1982;60(4):834-40.
[cited by applicant]
Goldman et al., A boost of BMP4 accelerates the commitment of human embryonic stem cells to the endothelial lineage. Stem Cells. Aug. 2009;27(8):1750-9.
[cited by applicant]
Golozoubova et al., Only UCP1 can mediate adaptive nonshivering thermogenesis in the cold. FASEB J. Sep. 2001;15(11):2048-50.
[cited by applicant]
Goumans et al., TGF-beta signaling in vascular biology and dysfunction. Cell Res. Jan. 2009;19(1):116-27.
[cited by applicant]
Grigolo et al., Transplantation of chondrocytes seeded on a hyaluronan derivative (hyaff-11) into cartilage defects in rabbits. Biomaterials. Sep. 2001;22(17):2417-24.
[cited by applicant]
Grützkau et al., Small but mighty: how the MACS-technology based on nanosized superparamagnetic particles has helped to analyze the immune system within the last 20 years. Cytometry A. Jul. 2010;77(7):643-7.
[cited by applicant]
Guduric-Fuchs et al., Selective extracellular vesicle-mediated export of an overlapping set of microRNAs from multiple cell types. BMC Genomics. Aug. 1, 2012;13:357. 14 pages.
[cited by applicant]
Haflidadóttir et al., Upregulation of miR-96 enhances cellular proliferation of prostate cancer cells through FOXO1. PLoS One. Aug. 12, 2013;8(8):e72400. 11 pages.
[cited by applicant]
Harms et al., Brown and beige fat: development, function and therapeutic potential. Nat Med. Oct. 2013;19(10):1252-63.
[cited by applicant]
Hassan et al., Encapsulation and 3D culture of human adipose-derived stem cells in an in-situ crosslinked hybrid hydrogel composed of PEG-based hyperbranched copolymer and hyaluronic acid. Stem Cell Res Ther. Mar. 21, 2…
[cited by applicant]
Hedman et al., Isolation of the pericellular matrix of human fibroblast cultures. J Cell Biol. Apr. 1979;81(1):83-91.
[cited by applicant]
Hemmrich et al., Implantation of preadipocyte-loaded hyaluronic acid-based scaffolds into nude mice to evaluate potential for soft tissue engineering. Biomaterials. Dec. 2005;26(34):7025-37.
[cited by applicant]
Heo et al., Spontaneous differentiation of mouse embryonic stem cells in vitro: characterization by global gene expression profiles. Biochem Biophys Res Commun. Jul. 15, 2005;332(4):1061-9.
[cited by applicant]
Herrera et al., Human liver stem cell-derived microvesicles accelerate hepatic regeneration in hepatectomized rats. J Cell Mol Med. Jun. 2010;14(6B):1605-18.
[cited by applicant]
Ho et al., Synthetic protein transduction domains: enhanced transduction potential in vitro and in vivo. Cancer Res. Jan. 15, 2001;61(2):474-7.
[cited by applicant]
Honda et al., Cartilage formation by cultured chondrocytes in a new scaffold made of poly(L-lactide-epsilon-caprolactone) sponge. J Oral Maxillofac Surg. Jul. 2000;58(7):767-75.
[cited by applicant]
Huber et al., Haemangioblast commitment is initiated in the primitive streak of the mouse embryo. Nature. Dec. 2, 2004;432(7017):625-30.
[cited by applicant]
Hynes et al., Micropatterning of 3D Microenvironments for Living Biosensor Applications. Biosensors (Basel). Mar. 2014;4(1):28-44.
[cited by applicant]
Hölig et al., Novel RGD lipopeptides for the targeting of liposomes to integrin-expressing endothelial and melanoma cells. Protein Eng Des Sel. May 2004;17(5):433-41.
[cited by applicant]
Hüttemann et al., Mice deleted for heart-type cytochrome c oxidase subunit 7a1 develop dilated cardiomyopathy. Mitochondrion. Mar. 2012;12(2):294-304.
[cited by applicant]
Ibrahim et al., Exosomes as critical agents of cardiac regeneration triggered by cell therapy. Stem Cell Reports. May 8, 2014;2(5):606-19.
[cited by applicant]
Inman et al., SB-431542 is a potent and specific inhibitor of transforming growth factor-beta superfamily type I activin receptor-like kinase (ALK) receptors ALK4, ALK5, and ALK7. Mol Pharmacol. Jul. 2002;62(1):65-74.
[cited by applicant]
Jackson et al., Recognizing and avoiding siRNA off-target effects for target identification and therapeutic application. Nat Rev Drug Discov. Jan. 2010;9(1):57-67.
[cited by applicant]
Jaiswal et al., Long-term multiple color imaging of live cells using quantum dot bioconjugates. Nat Biotechnol. Jan. 2003;21(1):47-51.
[cited by applicant]
Jakob et al., Role of microRNAs in stem/progenitor cells and cardiovascular repair. Cardiovasc Res. Mar. 15, 2012;93(4):614-22.
[cited by applicant]
James et al., An abundant perivascular source of stem cells for bone tissue engineering. Stem Cells Transl Med. Sep. 2012;1(9):673-84.
[cited by applicant]
James et al., Contribution of human embryonic stem cells to mouse blastocysts. Dev Biol. Jul. 1, 2006;295(1):90-102.
[cited by applicant]
James et al., Expansion and maintenance of human embryonic stem cell-derived endothelial cells by TGFbeta inhibition is Id1 dependent. Nat Biotechnol. Feb. 2010;28(2):161-6.
[cited by applicant]
Jankovic et al., Id1 restrains myeloid commitment, maintaining the self-renewal capacity of hematopoietic stem cells. Proc Natl Acad Sci U S A. Jan. 23, 2007;104(4):1260-5.
[cited by applicant]
Jeong et al., Nanovesicles engineered from ES cells for enhanced cell proliferation. Biomaterials. Nov. 2014;35(34):9302-10.
[cited by applicant]
Kane et al., Derivation of endothelial cells from human embryonic stem cells by directed differentiation: analysis of microRNA and angiogenesis in vitro and in vivo. Arterioscler Thromb Vasc Biol. Jul. 2010;30(7):1389-9…
[cited by applicant]
Kang et al., A self-enabling TGFbeta response coupled to stress signaling: Smad engages stress response factor ATF3 for Id1 repression in epithelial cells. Mol Cell. Apr. 2003;11(4):915-26.
[cited by applicant]
Karamanlidis et al., C/EBPbeta reprograms white 3T3-L1 preadipocytes to a Brown adipocyte pattern of gene expression. J Biol Chem. Aug. 24, 2007;282(34):24660-9.
[cited by applicant]
Kawamoto et al., Role of progenitor endothelial cells in cardiovascular disease and upcoming therapies. Catheter Cardiovasc Interv. Oct. 1, 2007;70(4):477-84.
[cited by applicant]
Kawasaki et al., Vascular Repair by Tissue-Resident Endothelial Progenitor Cells in Endotoxin-Induced Lung Injury. Am J Respir Cell Mol Biol. Oct. 2015;53(4):500-12.
[cited by applicant]
Kazantzis et al., PAZ6 cells constitute a representative model for human brown pre- adipocytes. Front Endocrinol (Lausanne). Feb. 2, 2012;3:13.
[cited by applicant]
Keller et al., Exosomes: from biogenesis and secretion to biological function. Immunol Lett. Nov. 15, 2006;107(2):102-8.
[cited by applicant]
Kelly et al., Signaling hierarchy regulating human endothelial cell development. Arterioscler Thromb Vasc Biol. May 2009;29(5):718-24.
[cited by applicant]
Khakoo et al., Endothelial progenitor cells. Annu Rev Med. 2005;56:79-101.
[cited by applicant]
Kim et al., Extracellular membrane vesicles from tumor cells promote angiogenesis via sphingomyelin. Cancer Res. Nov. 1, 2002;62(21):6312-7.
[cited by applicant]
King et al., Hypoxic enhancement of exosome release by breast cancer cells. BMC Cancer. Sep. 24, 2012;12:421. 10 pages.
[cited by applicant]
Korchagin, Neoplastic Diseases Reviews, Stem Cells. CancerLink.ru. 26 pages, (2011).
[cited by applicant]
Krosl et al., In vitro expansion of hematopoietic stem cells by recombinant TAT-HOXB4 protein. Nat Med. Nov. 2003;9(11):1428-32.
[cited by applicant]
Kucharzewska et al., Exosomes reflect the hypoxic status of glioma cells and mediate hypoxia-dependent activation of vascular cells during tumor development. PNAS. Apr. 30, 2013;110(18):7312-7317.
[cited by applicant]
Lai et al., Animal models of diabetic retinopathy: summary and comparison. J Diabetes Res. 2013;2013:106594. 29 pages.
[cited by applicant]
Laine et al., MicroRNAs miR-96, miR-124, and miR-199a regulate gene expression in human bone marrow-derived mesenchymal stem cells. J Cell Biochem. Aug. 2012;113(8):2687-95.
[cited by applicant]
Lanza et al., Human therapeutic cloning. Nat Med. Sep. 1999;5(9):975-7.
[cited by applicant]
Laping et al., Inhibition of transforming growth factor (TGF)-beta1-induced extracellular matrix with a novel inhibitor of the TGF-beta type I receptor kinase activity: SB-431542. Mol Pharmacol. Jul. 2002;62(1):58-64.
[cited by applicant]
Laposa, Stem cells for drug screening. J Cardiovasc Pharmacol. Sep. 2011;58(3):240-5.
[cited by applicant]
Le Grand et al., Six1 regulates stem cell repair potential and self-renewal during skeletal muscle regeneration. J Cell Biol. Sep. 3, 2012;198(5):815-32.
[cited by applicant]
Lee et al., Deletion of heart-type cytochrome c oxidase subunit 7a1 impairs skeletal muscle angiogenesis and oxidative phosphorylation. J Physiol. Oct. 15, 2012;590(20):5231-43.
[cited by applicant]
Lee et al., Exosomes mediate the cytoprotective action of mesenchymal stromal cells on hypoxia-induced pulmonary hypertension. Circulation. Nov. 27, 2012;126(22):2601-11.
[cited by applicant]
Lee et al., HLA-E is a major ligand for the natural killer inhibitory receptor CD94/NKG2A. Proc Natl Acad Sci U S A. Apr. 28, 1998;95(9):5199-204.
[cited by applicant]
Levenberg et al., Endothelial cells derived from human embryonic stem cells. Proc Natl Acad Sci U S A. Apr. 2, 2002;99(7):4391-6.
[cited by applicant]
Levenberg et al., Endothelial potential of human embryonic stem cells. Blood. Aug. 1, 2007;110(3):806-14.
[cited by applicant]
Li et al., Comparison of reporter gene and iron particle labeling for tracking fate of human embryonic stem cells and differentiated endothelial cells in living subjects. Stem Cells. Apr. 2008;26(4):864-73.
[cited by applicant]
Limbourg et al., Evaluation of postnatal arteriogenesis and angiogenesis in a mouse model of hind-limb ischemia. Nat Protoc. 2009;4(12):1737-48.
[cited by applicant]
Lin et al., Quantum dot imaging for embryonic stem cells. BMC Biotechnol. Oct. 9, 2007;7:67. 10 pages.
[cited by applicant]
Lin et al., Unregulated miR-96 induces cell proliferation in human breast cancer by downregulating transcriptional factor FOXO3a. PLoS One. Dec. 23, 2010;5(12):e15797. 10 pages.
[cited by applicant]
Liu et al., MiR-106b and MiR-15b modulate apoptosis and angiogenesis in myocardial infarction. Cell Physiol Biochem. 2012;29(5-6):851-62.
[cited by applicant]
Lopatina et al., Platelet-derived growth factor regulates the secretion of extracellular vesicles by adipose mesenchymal stem cells and enhances their angiogenic potential. Cell Commun Signal. Apr. 11, 2014;12:26. 12 pa…
[cited by applicant]
Lu et al., Generation of functional hemangioblasts from human embryonic stem cells. Nat Methods. Jun. 2007;4(6):501-9.
[cited by applicant]
Lu et al., Targeting of embryonic stem cells by peptide-conjugated quantum dots. PLoS One. Aug. 10, 2010;5(8):e12075. 10 pages.
[cited by applicant]
Lyden et al., Impaired recruitment of bone-marrow-derived endothelial and hematopoietic precursor cells blocks tumor angiogenesis and growth. Nat Med. Nov. 2001;7(11):1194-201.
[cited by applicant]
Mali et al., Improved efficiency and pace of generating induced pluripotent stem cells from human adult and fetal fibroblasts. Stem Cells. Aug. 2008;26(8):1998-2005.
[cited by applicant]
Martinez et al., Shed membrane microparticles from circulating and vascular cells in regulating vascular function. Am J Physiol Heart Circ Physiol. Mar. 2005;288(3):H1004-9.
[cited by applicant]
Molek et al., Peptide phage display as a tool for drug discovery: targeting membrane receptors. Molecules. Jan. 21, 2011;16(1):857-87.
[cited by applicant]
Nakagawa et al., Reprogramming of somatic cells to pluripotency. Adv Exp Med Biol. 2010;695:215-24.
[cited by applicant]
Nakashiba et al., Netrin-G1: a novel glycosyl phosphatidylinositol-linked mammalian netrin that is functionally divergent from classical netrins. J Neurosci. Sep. 1, 2000;20(17):6540-50.
[cited by applicant]
Naldini et al., In vivo gene delivery and stable transduction of nondividing cells by a lentiviral vector. Science. Apr. 12, 1996;272(5259):263-7.
[cited by applicant]
Nelson et al., Induced pluripotent stem cells: advances to applications. Stem Cells Cloning. Jan. 1, 2010;3:29-37.
[cited by applicant]
Nicoli et al., MicroRNA-mediated integration of haemodynamics and Vegf signalling during angiogenesis. Nature. Apr. 22, 2010;464(7292):1196-200.
[cited by applicant]
Niemelä et al., Molecular identification of PAL-E, a widely used endothelial-cell marker. Blood. Nov. 15, 2005;106(10):3405-9.
[cited by applicant]
Nishio et al., Production of functional classical brown adipocytes from human pluripotent stem cells using specific hemopoietin cocktail without gene transfer. Cell Metab. Sep. 5, 2012;16(3):394-406.
[cited by applicant]
Nonaka et al., Development of stabilin2+ endothelial cells from mouse embryonic stem cells by inhibition of TGFbeta/activin signaling. Biochem Biophys Res Commun. Oct. 17, 2008;375(2):256-60.
[cited by applicant]
Nourse et al., VEGF induces differentiation of functional endothelium from human embryonic stem cells: implications for tissue engineering. Arterioscler Thromb Vasc Biol. Jan. 2010;30(1):80-9.
[cited by applicant]
Odaka, Localization of mesenchymal cells in adult mouse thymus: their abnormal distribution in mice with disorganization of thymic medullary epithelium. J Histochem Cytochem. Apr. 2009;57(4):373-82.
[cited by applicant]
Ohshima et al., Let-7 microRNA family is selectively secreted into the extracellular environment via exosomes in a metastatic gastric cancer cell line. PLoS One. Oct. 8, 2010;5(10):e13247. 10 pages.
[cited by applicant]
Ong et al., Cross talk of combined gene and cell therapy in ischemic heart disease: role of exosomal microRNA transfer. Circulation. Sep. 9, 2014;130(11 Suppl 1):S60-9.
[cited by applicant]
Orlova et al., Functionality of endothelial cells and pericytes from human pluripotent stem cells demonstrated in cultured vascular plexus and zebrafish xenografts. Arterioscler Thromb Vasc Biol. Jan. 2014;34(1):177-86.
[cited by applicant]
Pankratz et al., MicroRNA-155 Exerts Cell-Specific Antiangiogenic but Proarteriogenic Effects During Adaptive Neovascularization. Circulation. May 5, 2015;131(18):1575-89.
[cited by applicant]
Patel et al., Poly(ethylene glycol) hydrogel system supports preadipocyte viability, adhesion, and proliferation. Tissue Eng. Sep.-Oct. 2005;11(9-10):1498-505.
[cited by applicant]
Peichev et al., Expression of VEGFR-2 and AC133 by circulating human CD34(+) cells identifies a population of functional endothelial precursors. Blood. Feb. 1, 2000;95(3):952-8.
[cited by applicant]
Perka et al., Joint cartilage repair with transplantation of embryonic chondrocytes embedded in collagen-fibrin matrices. Clin Exp Rheumatol. Jan.-Feb. 2000;18(1):13-22.
[cited by applicant]
Prestwich et al., The translational imperative: making cell therapy simple and effective. Acta Biomater. Dec. 2012;8(12):4200-7.
[cited by applicant]
Rafii et al., Cancer. A few to flip the angiogenic switch. Science. Jan. 11, 2008;319(5860):163-4.
[cited by applicant]
Ragni et al., Adipogenic potential in human mesenchymal stem cells strictly depends on adult or foetal tissue harvest. Int J Biochem Cell Biol. Nov. 2013;45(11):2456-66.
[cited by applicant]
Ramskold et al., An abundance of ubiquitously expressed genes revealed by tissue transcriptome sequence data. PLoS Comput Biol. 2009;5(12):e1000598, 11 pages.
[cited by applicant]
Rasmussen et al., TNFerade Biologic: preclinical toxicology of a novel adenovector with a radiation-inducible promoter, carrying the human tumor necrosis factor alpha gene. Cancer Gene Ther. Nov. 2002;9(11):951-7.
[cited by applicant]
Religa et al., Presence of bone marrow-derived circulating progenitor endothelial cells in the newly formed lymphatic vessels. Blood. Dec. 15, 2005;106(13):4184-90.
[cited by applicant]
Reubinoff et al., Embryonic stem cell lines from human blastocysts: somatic differentiation in vitro. Nat Biotechnol. Apr. 2000;18(4):399-404.
[cited by applicant]
Rhie et al., Implantation of Cultured Preadipocyte Using Chitosan/Alginate Sponge. Key Engineering Materials. 2007;342-343;349-352.
[cited by applicant]
Riolobos et al., HLA engineering of human pluripotent stem cells. Mol Ther. Jun. 2013;21(6):1232-41.
[cited by applicant]
Rong et al., A scalable approach to prevent teratoma formation of human embryonic stem cells. J Biol Chem. Sep. 21, 2012;287(39):32338-45.
[cited by applicant]
Rong et al., An effective approach to prevent immune rejection of human ESC-derived allografts. Cell Stem Cell. Jan. 2, 2014;14(1):121-30.
[cited by applicant]
Rosensteel et al., COL1A1 oligodeoxynucleotides decoy: biochemical and morphologic effects in an acute wound repair model. Exp Mol Pathol. Dec. 2010;89(3):307-13.
[cited by applicant]
Rosler et al., Long-term culture of human embryonic stem cells in feeder-free conditions. Dev Dyn. Feb. 2004;229(2):259-74.
[cited by applicant]
Rossig et al., Histone deacetylase activity is essential for the expression of HoxA9 and for endothelial commitment of progenitor cells. J Exp Med. Jun. 6, 2005;201(11):1825-35.
[cited by applicant]
Rudert et al., Bioartificial Cartilage. Cells Tissues Organs. 2000;167:95-105.
[cited by applicant]
Ruzinova et al., Id proteins in development, cell cycle and cancer. Trends Cell Biol. Aug. 2003;13(8):410-8.
[cited by applicant]
Sagare et al., Pericyte loss influences Alzheimer-like neurodegeneration in mice. Nat Commun. 2013;4:2932. 14 pages.
[cited by applicant]
Sahoo et al., Exosomes from human CD34(+) stem cells mediate their proangiogenic paracrine activity. Circ Res. Sep. 16, 2011;109(7):724-8.
[cited by applicant]
Salven et al., VEGFR-3 and CD133 identify a population of CD34+ lymphatic/vascular endothelial precursor cells. Blood. Jan. 1, 2003;101(1):168-72.
[cited by applicant]
Schnerch et al., Distinguishing between mouse and human pluripotent stem cell regulation: the best laid plans of mice and men. Stem Cells. Mar. 31, 2010;28(3):419-30.
[cited by applicant]
Schniedermann et al., Mouse lung contains endothelial progenitors with high capacity to form blood and lymphatic vessels. BMC Cell Biology. 2010;11(50):1-13.
[cited by applicant]