Preparation and use of a composition for prevention and mitigation of the effects of radiation
Plant extracts, compositions, pharmaceutical compositions and methods of making and using the same are provided herein. The compositions comprise γ-tocotrienol (GT3) and δ-tocotrienol (DT3) in ratios wherein the DT3 is predominate. The compositions are useful for radioprotection and radiomitigation in subjects in need thereof.
1. A method of treating a subject in need of a radioprotectant or for treatment of radiation exposure, the method comprising administering an effective amount of a tocotrienol composition comprising δ-tocotrienol and γ-tocotrienol in a ratio of between 2:1 and 10:1.
2. The method of claim 1 , wherein the tocotrienol composition comprises δ-tocotrienol and γ-tocotrienol in a ratio of between 5:1 and 9:1.
3. The method of claim 1 , wherein the tocotrienol composition comprises an annatto seed extract.
4. The method of claim 3 , wherein the annatto seed extract is prepared from a method comprising:
extracting oil from annatto seeds to produce an annatto seed oil, the annatto seed oil comprising δ-tocotrienol and γ-tocotrienol, and
enriching δ-tocotrienol or γ-tocotrienol from the annatto seed oil.
5. The method of claim 4 , wherein the method comprises enriching δ-tocotrienol and γ-tocotrienol from the annatto seed oil.
6. The method of claim 1 , wherein the tocotrienol composition comprises a total tocotrienol content of between 15% and 95%.
7. The method of claim 6 , wherein the total tocotrienol content is at least 50%.
8. The method of claim 1 , wherein the tocotrienol composition comprises a total tocotrienol content of at least 100 mg/mL.
9. The method of claim 8 , wherein the total tocotrienol content is at least 500 mg/mL.
10. The method of claim 1 , wherein the tocotrienol composition comprises a tocopherol content of less than 5%.
11. The method of claim 10 , wherein the tocopherol content is less than 1%.
12. The method of claim 1 , wherein the tocotrienol composition comprises a total tocotrienol content of between 15% and 95% and a tocopherol content of less than 5%.
13. The method of claim 2 , wherein the tocotrienol composition comprises an annatto seed extract having δ-tocotrienol and γ-tocotrienol in a ratio of between 5:1 and 9:1.
14. The method of claim 1 , wherein the tocotrienol composition is a pharmaceutical composition further comprising a pharmaceutically acceptable carrier.
15. The method of claim 14 , wherein the pharmaceutical composition comprises a dosage form having a total tocotrienol content of least 1 g.
16. The method of claim 15 , wherein the pharmaceutical composition comprises an annatto seed extract.
17. The method of claim 16 , wherein the annatto seed extract is prepared from a method comprising:
extracting oil from annatto seeds to produce an annatto seed oil, the annatto seed oil comprising δ-tocotrienol and γ-tocotrienol, and
enriching δ-tocotrienol or γ-tocotrienol from the annatto seed oil.