Salt formulations for the fermentation of marine microorganisms
Growth medium are disclosed for use in fermenting a marine microorganism. The medium comprise Potassium, Calcium, Strontium, Borate and Fluoride at specific concentrations. Alternatively, the growth medium comprises cobalt at specified concentrations or comprises vitamin B 12 at specified concentrations. Methods of producing certain desired compound by fermentation of a marine microorganism are also disclosed.
1. A salt formulation for use in fermenting a marine microorganism, the formulation comprising:
(a) NaCl in the salt formulation at a mass percent between 92% and 76%;
(c) KCl in the salt formulation at a mass percent between 5% and 1.50%;
(d) CaCl 2 ⋅H 2 O in the salt formulation at a mass percent between 3% and 1%;
(e) SrCl 2 in the salt formulation at a mass percent between 0.10% and 0.025%;
(f) H 3 BO 3 ⋅7H 2 O in the salt formulation at a mass percent between 0.20% and 0.04%; and
(g) NaF in the salt formulation at a mass percent between 0.05% and 0.005%.
2. The salt formulation of claim 1 , further comprising CaCO 3 at a mass percent between 5% and 1%.
3. The salt formulation of claim 1 , further comprising CoCl 2 ⋅6H 2 O.
4. The salt formulation of claim 1 , further comprising KBr at a mass percent between 3% and 0.10%.
5. The salt formulation of claim 1 , further comprising Fe 2 (SO 4 ) 3 .
6. A salt formulation for use in fermenting a marine microorganism, the formulation comprising:
(a) Na 2 SO 4 at a mass percent between 85% and 72%;
(c) KCl at a mass percent between 5% and 3%;
(d) CaCl 2 ⋅H 2 O at a mass percent between 3% and 1%;
(e) SrCl 2 at a mass percent between 0.15% and 0.05%;
(f) H 3 BO 3 ⋅7H 2 O at a mass percent between 0.30% and 0.10%; and
(g) NaF at a mass percent between 0.03% and 0.01%.
7. The salt formulation of claim 6 , further comprising CaCO 3 at a mass percent between 20% and 0.5%.
8. The salt formulation of claim 6 , further comprising CoCl 2 ⋅6H 2 O.
9. The salt formulation of claim 6 , further comprising KBr at a mass percent between 3% and 0.10%.
10. The salt formulation of claim 6 , further comprising Fe 2 (SO 4 ) 3 .
11. A salt formulation for use in fermenting a marine microorganism, the formulation comprising:
(a) KCl at a mass percent between 90% and 75%;
(b) MgSO 4 at a mass percent between 20% and 5%
(c) CaCl 2 at a mass percent between 5% and 3%;
(d) SrCl 2 at a mass percent between 0.3% and 0.03%;
(e) H 3 BO 3 at a mass percent between 0.4% and 0.04%; and
(f) NaF at a mass percent between 0.03% and 0.01%.