Malaria antigens and methods of use
The invention is directed to a composition comprising one or more polypeptides or one or more nucleic acid sequences that can induce a protective immune response against Plasmodium species that infect humans. The invention also is directed to a method of using such compositions to induce a protective immune response against a Plasmodium parasite in a mammal.
1. A composition comprising a pharmaceutically acceptable carrier and one or more isolated nucleic acid sequences, wherein the one or more isolated nucleic acid sequences are selected from the group consisting of SEQ ID NOs: 27-29.
2. The composition of claim 1 , wherein the one or more isolated nucleic acid sequences are present in a vector.
3. The composition of claim 2 , wherein the vector is an adenoviral vector.
4. The composition of claim 3 , wherein the adenoviral vector is a human adenoviral vector or a gorilla adenoviral vector.
5. The composition of claim 3 , wherein the adenoviral vector requires complementation of a deficiency in one or more early regions of the adenoviral genome for propagation and does not require complementation of any other deficiency of the adenoviral genome for propagation.
6. The composition of claim 5 , wherein the one or more early regions are selected from the group consisting of the E1 region, the E2 region, and the E4region of the adenovirus genome.
7. The composition of claim 5 , wherein the adenoviral vector requires complementation of a deficiency in the E1 region of the adenoviral genome for propagation and does not require complementation of any other deficiency of the adenoviral genome for propagation.
8. The composition of claim 5 , wherein the adenoviral vector requires complementation of a deficiency in the E1A region or the E1B region of the adenoviral genome for propagation and does not require complementation of any other deficiency of the adenoviral genome for propagation.
9. The composition of claim 5 , wherein the adenoviral vector requires complementation of a deficiency in the E4region of the adenoviral genome for propagation and does not require complementation of any other deficiency of the adenoviral genome for propagation.
10. The composition of claim 5 , wherein the adenoviral vector requires complementation of a deficiency in the El region of the adenoviral genome and a deficiency in the E4 region of the adenoviral genome for propagation and does not require complementation of any other deficiency of the adenoviral genome for propagation.
11. A method of inducing a protective immune response against a Plasmodium parasite in a mammal, which method comprises administering to the mammal the composition of claim 1 , whereupon a protective immune response against the Plasmodium parasite in the mammal is induced.
12. The method of claim 11 , wherein the mammal is a human.
13. The method of claim 11 , wherein the Plasmodium parasite is Plasmodium falciparum or Plasmodium vivax .
14. The method of claim 11 , wherein the one or more isolated nucleic acid sequences are present in a vector.
15. The method of claim 14 , wherein the vector is an adenoviral vector.
16. The method of claim 15 , wherein the adenoviral vector is a human adenoviral vector or a gorilla adenoviral vector.
17. The method of claim 15 , wherein the adenoviral vector requires complementation of a deficiency in one or more early regions of the adenoviral genome for propagation and does not require complementation of any other deficiency of tare adenoviral genome for propagation.
18. The composition of claim 1 , comprising a pharmaceutically acceptable carrier and the isolated nucleic acid sequence of SEQ II) NO: 27.
19. The composition of claim 1 , comprising a pharmaceutically acceptable carrier and the isolated nucleic acid sequence of SEQ ID NO: 28.
20. The composition of claim 1 , comprising a pharmaceutically acceptable carrier and the isolated nucleic acid sequence of SEQ II) NO: 29.