Microfluidic Device

1 . A microfluidic device comprising:

a single sheet of paper comprising a main channel, at least two fluid transfer channels fluidically coupled to the main channel, and a separate diagnostic area fluidically coupled to each fluid transfer channel;

wax on a surface of the paper, the wax defining boundaries of the main channel, the at least two fluid transfer channels, and the diagnostic areas;

a wax backing on an opposite surface of the paper; and

at least one liver function assay reagent provided within at least one of the diagnostic areas.

2 . (canceled)

3 . The microfluidic device of claim 1 , further comprising a filter fluidically coupled to the main channel.

4 . The microfluidic device of claim 1 , wherein each fluid transfer channel comprises an anti-coagulant component.

5 . The microfluidic device of claim 4 , wherein the anti-coagulant component is EDTA.

6 . The microfluidic device of claim 4 , wherein the anti-coagulant component is heparin.

7 . The microfluidic device of claim 1 , wherein the at least one liver function assay reagent includes one or more dyes.

8 . The microfluidic device of claim 1 , wherein the hydrophilic substrate includes three fluid transfer channels fluidically coupled to the main channel, and wherein each of the three fluid channels terminates in a separate diagnostic area.

9 . The microfluidic device of claim 8 , wherein each diagnostic area includes a different liver function assay reagent.

10 . The microfluidic device of claim 8 , wherein a first and second fluid transfer channel have the same length, and wherein a third fluid transfer channel has a length which is less than either the first or second fluid transfer channels.

11 . A microfluidic device comprising:

at least two assay arrays, each assay array comprising:

a hydrophilic substrate, the hydrophilic substrate comprising a main channel, at least two fluid transfer channels fluidically coupled to the main channel, wherein each of the at least two fluid transfer channels terminates in a separate diagnostic area fluidically coupled to each fluid transfer channel,

wax on a surface of the hydrophilic substrate, the wax defining the boundaries of the main channel, the at least two fluid transfer channels, and the diagnostic areas;

a wax backing on an opposite surface of the hydrophilic substrate;

at least one liver function assay reagent provided within at least one of the diagnostic areas; and

a calibration region;

wherein the wax backings together provide a continuous coating of the opposite surface of the hydrophilic substrate.

12 . The microfluidic device of claim 11 , wherein each fluid transfer channel comprises an anti-coagulant component.

13 . The microfluidic device of claim 11 , wherein the liver function assay reagent comprises a stabilizer and at least one dye.

14 . The microfluidic device of claim 11 , wherein the liver function assay reagent selectively associates with albumin, alkaline phosphatase, alanine transaminase, aspartate transaminase, total bilirubin, and total protein.

15 . The microfluidic device of claim 11 , wherein the device is configured such that quantities of albumin, alkaline phosphatase, alanine transaminase, aspartate transaminase, total bilirubin, and total protein are detected.

16 . A micro fluidic device comprising: a hydrophilic substrate having at least six fluid transfer channels, wherein each of the six fluid transfer channels terminate in a discrete diagnostic area, wherein each of the at least six fluid transfer channels and discrete diagnostic areas are defined by wax boundaries on the hydrophilic substrate; and wherein a first discrete diagnostic area includes a first assay reagent that selectively associates with albumin, a second discrete diagnostic area includes a second assay reagent that selectively associates with alkaline phosphatase, a third discrete diagnostic area includes third assay reagent that selectively associates with alanine transaminase, a fourth discrete diagnostic area includes a fourth assay reagent that selectively associates with aspartate transaminase, a fifth discrete diagnostic area includes a fifth assay reagent that selectively associates with total bilirubin, and a sixth discrete diagnostic area includes a sixth assay reagent that selectively associates with total protein.

17 . The microfluidic device of claim 16 , wherein each of the at least six fluid transfer channels includes an anti-coagulant.

18 . The microfluidic device of claim 16 , wherein at least two of the discrete diagnostic areas include different dyes.

19 . The microfluidic device of claim 16 , wherein at least one of the assay arrays includes a calibration region.

20 . The microfluidic device of claim 16 , wherein the microfluidic device comprises at least two separate assay arrays.

21 . The microfluidic device of claim 1 , wherein the microfluidic device further comprises a calibration region.