AAV's and uses thereof
The invention in some aspects relates to recombinant adeno-associated viruses having distinct tissue targeting capabilities. In some aspects, the invention relates to gene transfer methods using the recombinant adeno-associate viruses. In some aspects, the invention relates to isolated AAV capsid proteins and isolated nucleic acids encoding the same.
1. A recombinant adeno-associated virus (rAAV) comprising a nucleic acid encoding a heterologous transgene and an AAV capsid, wherein the AAV capsid comprises a protein comprising an amino acid sequence selected from SEQ ID NOs.: 96, 98, 100, and 111.
2. The rAAV of claim 1 , wherein the heterologous transgene comprises a tissue-specific promoter operably linked to a coding sequence.
3. The rAAV of claim 2 , wherein the coding sequence encodes a therapeutic protein.
4. The rAAV of claim 3 , wherein the therapeutic protein is aromatic amino acid decarboxylase (AADC).
5. A composition comprising the rAAV of claim 1 .
6. The composition of claim 5 , further comprising a pharmaceutically acceptable carrier.
7. The rAAV of claim 1 , wherein the AAV capsid protein comprises the amino acid sequence set forth in SEQ ID NO.: 96.
8. A composition comprising the rAAV of claim 7 and a pharmaceutically acceptable carrier.
9. The rAAV of claim 1 , wherein the AAV capsid protein comprises the amino acid sequence set forth in SEQ ID NO.: 98.
10. A composition comprising the rAAV of claim 9 and a pharmaceutically acceptable carrier.
11. The rAAV of claim 1 , wherein the AAV capsid protein comprises the amino acid sequence set forth in SEQ ID NO.: 100.
12. A composition comprising the rAAV of claim 11 and a pharmaceutically acceptable carrier.
13. The rAAV of claim 1 , wherein the AAV capsid protein comprises the amino acid sequence set forth in SEQ ID NO.: 111.
14. A composition comprising the rAAV of claim 13 and a pharmaceutically acceptable carrier.
15. The rAAV of claim 3 , wherein the AAV capsid protein comprises the amino acid sequence set forth in SEQ ID NO.: 96.
16. The rAAV of claim 3 , wherein the AAV capsid protein comprises the amino acid sequence set forth in SEQ ID NO.: 98.
17. The rAAV of claim 3 , wherein the AAV capsid protein comprises the amino acid sequence set forth in SEQ ID NO.: 100.
18. The rAAV of claim 3 , wherein the AAV capsid protein comprises the amino acid sequence set forth in SEQ ID NO.: 111.