Method and electronic device for determining the concentration of an analyte
A method is provided for determining, the presence and concentration of an analyte by contacting the sample with a solution comprising: magnetic beads, a capture probe capable of binding the analyte, a reporter probe and cellulose, whereby, if the analyte is present, an MB-analyte-reporter-cellulase sandwich is formed; and then contacting the solution comprising the sandwich with an electrode covered with an electrically insulating layer comprising or consisting of cellulose and/or a cellulose derivative, wherein the MB-analyte-reporter-cellulase sandwich leads to degradation of the insulating layer thereby causing a measurable change in electrical properties at the electrode surface, wherein the change in electrical properties is a function of the amount of analyte in the sample. Devices and biosensor applying the method are also provided.
1. A device for measuring the concentration of an analyte, wherein said device comprises an electrode covered with an electrically insulating layer comprising or consisting of cellulose or cellulose derivatives, wherein said covered electrode is embedded in a solution comprising magnetic beads, a capture probe bound to a magnetic bead, and a reporter probe bound to cellulase.
2. The device according to claim 1 , wherein said analyte is an oligonucleotide, a protein or a cell.
3. The device according to claim 1 , wherein said capture probe is an oligonucleotide, a biotinylated oligonucleotide, biotinylated DNA, an antibody or an aptamer.
4. The device according to claim 1 , wherein said reporter probe is an oligonucleotide, a biotinylated oligonucleotide, biotinylated DNA, an antibody or an aptamer.
5. The device according to claim 1 , wherein said insulating layer comprises or consists of nitrocellulose.
6. The device according to claim 1 , wherein said device is a biosensor.
7. A kit for measuring the concentration of an analyte comprising an electrode covered with an electrically insulating layer comprising or consisting of cellulose or cellulose derivatives, magnetic beads, a capture probe bound to a magnetic bead, a reporter probe bound to cellulase, wherein:
said analyte is an oligonucleotide, a protein or a cell;
said capture probe is an oligonucleotide, a biotinylated oligonucleotide, biotinylated DNA, an antibody or an aptamer;
said reporter probe is an oligonucleotide, a biotinylated oligonucleotide, biotinylated DNA, an antibody or an aptamer; and
said insulating layer comprises or consists of nitrocellulose.