Compositions and vaccines comprising vesicles and methods of using the same
The disclosure relates to compositions, pharmaceutical compositions, vaccines, and methods of making and using the same. The compositions of the disclosure are useful to stimulate an antigen-specific immune response and, in some embodiments, a protective immune response in an animal after challenge to pathogens. The compositions of the disclosure are also useful for treatment and prevention of disease in a subject such as cancer.
1. A method of generating an antibody response against Neisseria gonorrhoeae in a subject in need thereof, comprising administering to the subject a therapeutically effective amount of a composition comprising a catanionic surfactant vesicle;
wherein the catanionic surfactant vesicle comprises a unilamellar bilayer comprising a mixture of oppositely charged single-tailed surfactants and one or a plurality of Neisseria gonorrhoeae immunogens from whole Neisseria gonorrhoeae cell extract;
wherein the one or plurality of Neisseria gonorrhoeae immunogens comprise at least one membrane-bound sugar, or at least one membrane-bound polypeptide or at least one combination of a membrane-bound sugar and membrane-bound polypeptide; and wherein the membrane-bound sugar, the membrane-bound polypeptide, or the combination of the membrane-bound sugar and membrane-bound polypeptide is positioned at least partially in the unilamellar bilayer;
wherein the catanionic surfactant vesicle comprises bacterial lipooligosaccharide (LOS) or lipopolysaccharide (LPS) and one or a plurality of Neisseria gonorrhoeae lipoproteins or Neisseria gonorrhoeae membrane-bound protein; and
wherein the catanionic surfactant vesicle comprises a percentage of carbohydrate from about 0% to about 25% by weight.
2. The method of claim 1 , wherein the catanionic surfactant vesicle comprises a percentage of carbohydrate from about 0.1% to about 16% by weight.
3. The method of claim 1 , wherein the one or plurality of Neisseria gonorrhoeae immunogens comprise one or a combination of pilin, porA, porB, OPA, DnaK, Tul4, and FopA.
4. The method of claim 1 , wherein the one or plurality of Neisseria gonorrhoeae immunogens comprises Pan HLA-DR reactive epitope (PADRE) individually or conjugated to one or a plurality of membrane-bound sugar molecules.
5. The method of claim 1 , wherein the catanionic surfactant vesicle is lyophilized.