Granted Patent B2

US 12,303,810 · App. 16/033,126 · Granted May 20, 2025

Inventors: Attila Herczeg (Southborough, MA); Bengt G. Persson (Boston, MA); Julie-Anne Burdick (Hudson, MA)

Assignee: WaterSep BioSeparations LLC

B01D29/908B01D61/145B01D61/147B01D61/22B01D63/034B01D63/069

Abstract

Claims (39)

1. A single pass cross flow filtration system for a viscous liquid comprising biopolymers sensitive to shear rate through controlling a permeate flux, comprising:

a fluid reservoir fluidly connected to a means for urging fluid to a first filtration segment of a filtration module, the filtration module comprising two or more filtration segments fluidly connected in series, each having an upstream side and a downstream side, and each having a retentate channel and a permeate channel;

at least one pump and at least one valve in the retentate channel between respective ones of the two or more filtration segments;

at least one pump mounted in the permeate channel of each of the two or more filtrations segments, and

a fluid line segment for exiting permeate from all permeate channels to a reservoir for deposition, the fluid line segment comprising at least one valve positioned between the entries of permeate channels of two filtration segments,

wherein each filtration segment has a selected length and comprises hollow fiber filter membranes, the hollow fiber filter membranes of each filtration segment having a selected lumen diameter,

wherein at least one selected lumen diameter differs from another selected lumen diameter, and provided that the two or more filtration segments are arranged such that for a given filtration segment having a selected lumen diameter, no filtration segment on the upstream side of the given filtration segment has a selected lumen diameter that is larger,

wherein the at least one pump and the at least one valve in the respective retentate channel are positioned between filtration segments with the differing lumen diameters, thereby regulating the flow of retentate from the segment on the upstream side into the segment on the downstream side, and

wherein the means for urging fluid to the first filtration segment, all pumps mounted in the permeate channel of each of the two or more filtration segments, and the at least one valve in the fluid line segment for exiting permeate are controlled by a processor to control the permeate flux in the permeate channels of the two or more filtration segments.

2. The single pass cross flow filtration system of claim 1 , wherein the selected lengths of the two or more filtration segments are from about 12.5 cm to about 800 cm.

3. The single pass cross flow filtration system of claim 1 , wherein the selected lengths of the two or more filtration segments are chosen from about 30.5 cm, about 61 cm, and about 104 cm.

4. The single pass cross flow filtration system of claim 1 , wherein the two or more filtration segments each have a filtration segment housing inner diameter, and wherein each filtration segment housing inner diameter is chosen from about 0.5 cm to about 26 cm.

5. The single pass cross flow filtration system of claim 1 , wherein the two or more filtration segments each have a filtration segment housing inner diameter, and wherein each filtration segment housing inner diameter is chosen from about 0.95 cm to about 16 cm.

6. The single pass cross flow filtration system of claim 1 , wherein the selected lumen diameters are about 0.1 mm to about 5.0 mm.

7. The single pass cross flow filtration system of claim 1 wherein the hollow fiber filter membranes have pore sizes from about 1 nanometers to about 500 micrometers.

8. The single pass cross flow filtration system of claim 1 , wherein the at least one pump mounted in the permeate channel of each of the two or more filtration segments is configured to provide a back pressure in the permeate channel.

9. The single pass cross flow filtration system of claim 1 , wherein for each filtration segment that is located on a downstream side of an upstream filtration segment of the two or more filtration segments, the selected lumen diameter of the filtration segment on the downstream side is larger than the selected lumen diameter of the upstream filtration segment.

10. A method of single pass cross-flow filtration of a viscous liquid comprising biopolymers sensitive to shear rate for controlling permeate flux, comprising:

a. providing a fluid to be filtered;

b. providing a fluid reservoir containing the fluid to be filtered, the fluid reservoir being fluidly connected to a means for urging fluid to a first filtration segment of a filtration module;

c. the filtration module comprising two or more filtration segments fluidly connected in series, each filtration segment having an upstream side and a downstream side, and each having a retentate channel and a permeate channel; wherein each filtration segment comprises hollow fiber filter membranes;

d. adjusting the permeate flux to reach desired values of axial velocity, shear rate and its associated shear stress, axial pressure drops and transmembrane pressure drops by providing a configuration of components, the configuration of components comprising:

i. two or more filtration segments, each of the two or more filtration segments having a selected length;

ii. one or more hollow fiber filter membranes in each filtration segment, the one or more hollow fiber filter membranes having a selected lumen diameter, wherein at least one of the selected lumen diameters differs from the others, and provided that the two or more filtration segments are arranged such that for a given filtration segment having a selected lumen diameter, no filtration segment on the upstream side of the given filtration segment has a selected lumen diameter that is larger;

iii. at least one pump and at least one valve in the retentate channel of respective ones of the two or more filtration segments, the at least one pump and the at least one valve being positioned between two filtration segments with the differing lumen diameters;

iv. at least one pump mounted in the permeate channel of each of the two or more filtration segments;

v. a fluid line segment for exiting permeate from all permeate channels to a reservoir for depositing, which comprises at least one valve positioned between the entries of permeate channels of two filtrations segments and

e. filtering a fluid mixture through the filtration module to obtain a retentate and a permeate,

wherein the means for urging the fluid to the first filtration segment, all pumps mounted in the permeate channel of each of the two or more filtration segments, and the at least one valve in the fluid line segment for exiting permeate are controlled by a processor to control the permeate flux in the permeate channels of the two or more filtration segments.

11. The method of claim 10 , wherein the selected lengths of each of the two or more filtration segments is chosen to be from about 4 inches to about 100 inches.

12. The method of claim 10 , wherein the selected lengths of the two or more filtration segments are chosen from about 12 inches, about 24 inches, and about 41 inches.

13. The method of claim 10 , wherein the two or more filtration segments each have a filtration segment housing inner diameter, and wherein each filtration segment housing inner diameter is chosen from about 0.5 cm to about 26 cm.

14. The method of claim 10 , wherein the selected lumen diameters are about 0.1 mm to about 5 mm.

15. The method of claim 10 , wherein the fluid to be filtered comprises a viscosity lowering agent chosen from proline, histidine, lysine, arginine, glutamic acid, betaine, glutamine, asparagine, imidazole and salts thereof.

16. The method of claim 10 wherein the hollow fiber filter membranes have pore sizes from about 10 nanometers to about 500 micrometers.

17. The method of claim 10 , further comprising operating the at least one pump mounted in the permeate channel of each of the two or more filtration segments to provide a back pressure in the permeate channel.

18. The method of claim 17 , further comprising periodically restricting the flow of the permeate by closing off the permeate channel.

19. The method of claim 10 , wherein for each filtration segment that is located on a downstream side of an upstream filtration segment of the two or more filtration segments, the selected lumen diameter of the filtration segment on the downstream side is larger than the selected lumen diameter of the upstream filtration segment.

20. The method of claim 10 , wherein the shear rate within the one or more hollow fiber filter is maintained below 2300 sec −1 .

Assignments (2)

Continuity (1)

References Cited (16)