Bacillus microbial terroir for pathogen control in swine
Disclosed are methods of administering one or more Bacillus subtilis strains to swine. The Bacillus subtilis strains that are administered include 747 (NRRL B-67257), 1104 (NRRL B-67258), 1541 (NRRL B-67260), 1781 (NRRL B-67259), 2018 (NRRL B-67261), and BS1999 (NRRL B-67318). The Bacillus strains improve bacterial homeostasis in the gastrointestinal tract by inhibiting bacterial pathogens such as E. coli, Clostridium, Salmonella , and Streptococcus . Administering the Bacillus strains also improves performance such as weight gain and feed conversion. Useful combinations of Bacillus strains and methods of using one or more Bacillus strains are also provided.
1. A direct fed microbial composition comprising
(a) an isolated Bacillus strain, wherein the isolated Bacillus strain is chosen from at least one of strains Bacillus subtilis 747, deposited as NRRL B-67257; Bacillus subtilis 1104, deposited as NRRL B-67258; Bacillus subtilis 1541, deposited as NRRL B-67260; Bacillus subtilis 1781, deposited as NRRL B-67259; Bacillus subtilis 2018, deposited as NRRL B-67261; and Bacillus subtilis 1999, deposited as NRRL B-67318; and
(b) a preservative;
wherein said isolated Bacillus strain is a powdered lyophilized isolated Bacillus strain, and
wherein the composition inhibits at least one pathogen selected from Escherichia coli, Salmonella, Clostridia , and Streptococcus suis by at least 85% overall in vitro.
2. The composition of claim 1 , wherein the composition comprises a combination of at least two isolated Bacillus strains chosen from strains Bacillus subtilis 747, Bacillus subtilis 1104, Bacillus subtilis 1541, Bacillus subtilis 1781, Bacillus subtilis 2018, and Bacillus subtilis 1999.
3. The composition of claim 1 , further comprising a carrier.
4. The composition of claim 1 , further comprising a cryoprotectant disposed about the isolated Bacillus strain, and wherein said powdered lyophilized isolated Bacillus strain comprises Bacillus spores.
5. The composition of claim 1 , further comprising an animal feed.
6. The composition of claim 5 , wherein the composition has a concentration of the isolated Bacillus strain in the composition of between about 1×10 5 CFU/g of feed and about 1×10 6 CFU/g of feed.
7. The composition of claim 6 , wherein the composition has a concentration of the isolated Bacillus strain in the composition of about 3.75×10 5 CFU/g of feed.
8. The composition of claim 1 , wherein the effective amount of said direct fed microbial composition ingested by the swine per day comprises a concentration of the isolated Bacillus strain of between about 1×10 6 CFU/swine and about 1×10 9 CFU/swine.
9. The composition of claim 1 , wherein the composition improves the fecal score of the swine at seven days post weaning, wherein the swine ingested the effective amount of said direct fed microbial composition between zero days and seven days post weaning from the sow.
10. The composition of claim 1 , wherein the composition improves the average fecal score of the swine during an initial 14 days of a nursery period, wherein the swine ingested the effective amount of said direct fed microbial composition between zero days and 14 days post weaning from the sow.
11. The composition of claim 1 , wherein the composition further inhibits Escherichia coli in the gastrointestinal tract of the swine at least seven days after the swine ingested the effective amount of said direct fed microbial composition.
12. The composition of claim 1 , wherein the composition further inhibits Salmonella in the gastrointestinal tract of the swine at least seven days after the swine ingested the effective amount of said direct fed microbial composition.
13. The composition of claim 1 , wherein the composition further inhibits Clostridia in the gastrointestinal tract of the swine at least seven days after the swine ingested the effective amount of said direct fed microbial composition.
14. The composition of claim 1 , wherein the composition inhibits Streptococcus suis in the gastrointestinal tract of the swine at least seven days after the swine ingested the effective amount of said direct fed microbial composition.
15. A direct fed microbial composition comprising:
a cryoprotectant disposed about a powdered lyophilized isolated Bacillus strain of spores chosen from at least one of: Bacillus subtilis 747, Bacillus subtilis 1104, Bacillus subtilis 1541, Bacillus subtilis 1781, Bacillus subtilis 2018, and Bacillus subtilis 1999, and
a preservative,
wherein said direct fed microbial composition improves performance of a swine that has ingested the direct fed microbial composition in an effective amount, wherein the effective amount of said direct fed microbial composition comprises a concentration of the isolated Bacillus strain of between about 1×10 6 CFU/swine/day and about 1×10 9 CFU/swine/day.
16. The direct fed microbial composition of claim 15 , wherein the effective amount of said direct fed microbial composition inhibits at least one pathogen selected from Escherichia coli, Salmonella, Clostridia , and Streptococcus suis in the gastrointestinal tract of the swine.