Smallpox vaccine for cancer treatment
Disclosed herein are methods and compositions related to therapy for cancer. More specifically, the disclosed methods and compositions are related to the use of smallpox vaccine to induce an effective anti-tumor immune response.
1. A method for treating a solid tumor or hematologic malignancy in a subject, comprising administering to the subject an oncolytic virus in a composition comprising adipose stromal cells, wherein:
prior to administration, the virus had been cultured with the adipose stromal cells; and
the virus is selected from among a poxvirus, adenovirus, herpes simplex virus, Newcastle disease virus, vesicular stomatitis virus, reovirus, cytomegalovirus (CMV), and lentivirus.
2. The method of claim 1 , wherein the oncolytic virus is a vaccinia virus.
3. The method of claim 1 , wherein the oncolytic virus does not comprise heterologous nucleic acid that encodes a heterologous product.
4. A method for treating a solid tumor or hematologic malignancy in a subject, comprising administering to the subject an oncolytic virus in a composition comprising adipose stromal cells, wherein:
the virus is a vaccinia virus; and
the virus is incubated with the composition comprising the adipose stromal cells for 2 to 4 hours prior to administration.
5. The method of claim 4 , wherein the vaccinia virus is an attenuated New York City Board of Health (NYCBOH) strain of vaccinia virus.
6. The method of claim 5 , wherein the NYCBOH strain of vaccinia virus has all of the identifying characteristics of the strain deposited as ATCC VR-118 or CJ-MVB-SPX.
7. The method of claim 4 , wherein the vaccinia virus is selected from among Dryvax, ACAM1000, ACAM2000, Lister, EM63, LIVP, Tian Tan, Copenhagen, Western Reserve, Modified Vaccinia Ankara (MVA), New York City Board of Health (NYCBOH), Dairen, Ikeda, LC16M8, Tashkent, Wyeth, IHD-J, IHD-W, Brighton, Dairen I, and Connaught strains.
8. The method of claim 1 , wherein the solid tumor or hematologic malignancy is a solid tumor that is a glioblastoma, breast carcinoma, lung carcinoma, pancreatic adenocarcinoma, prostate carcinoma, colon carcinoma, ovarian carcinoma, neuroblastoma, central nervous system tumor, or melanoma.
9. The method of claim 1 , wherein the oncolytic virus encodes a heterologous gene product.
10. The method of claim 1 , wherein the subject is human.
11. The method of claim 10 , wherein the subject is a pediatric patient.
12. The method of claim 1 , wherein the adipose stromal cells are in an adipose-derived stromal vascular fraction (SVF).
13. The method of claim 12 , wherein the adipose-derived SVF is autologous to the subject.
14. The method of claim 12 , wherein the adipose-derived SVF is allogeneic to the subject.
15. The method of claim 4 , wherein the vaccinia virus is ACAM1000 or ACAM2000.
16. The method of claim 15 , wherein the vaccinia virus is ACAM2000.
17. The method of claim 4 , wherein the solid tumor or hematologic malignancy is a solid tumor that is a glioblastoma, breast carcinoma, lung carcinoma, pancreatic adenocarcinoma, prostate carcinoma, colon carcinoma, ovarian carcinoma, neuroblastoma, central nervous system tumor, or melanoma.
18. A method of treating a solid tumor or a hematological malignancy in a subject, comprising administering to the subject a composition comprising a smallpox vaccine and a carrier cell for treating the solid tumor or hematological malignancy, wherein:
the smallpox vaccine and the carrier cell are cultured together in vitro prior to administration to the subject; and
the carrier cell is an immune cell.
19. The method of claim 18 , wherein the immune cell is a lymphocyte.
20. The method of claim 19 , wherein the lymphocyte is a T cell.
21. The method of claim 18 , wherein the smallpox vaccine is selected from among Dryvax, ACAM1000, ACAM2000, Lister, EM63, LIVP, Tian Tan, Copenhagen, Western Reserve, Modified Vaccinia Ankara (MVA), New York City Board of Health, Dairen, Ikeda, LC16M8, Tashkent, Wyeth, IHD-J, IHD-W, Brighton, Dairen I, and Connaught strains.
22. The method of claim 18 , wherein the smallpox vaccine is ACAM1000 or ACAM2000.
23. The method of claim 18 , wherein the solid tumor or hematological malignancy is a solid tumor that is a glioblastoma, pancreatic adenocarcinoma, breast carcinoma, lung carcinoma, prostate carcinoma, colon carcinoma, ovarian carcinoma, neuroblastoma, central nervous system tumor, or melanoma.
24. The method of claim 4 , wherein the oncolytic virus encodes a heterologous gene product.
25. The method of claim 4 , wherein the subject is human.
26. The method of claim 4 , wherein the composition comprising the adipose stromal cells is an adipose-derived stromal vascular fraction (SVF).
27. The method of claim 26 , wherein the adipose-derived SVF is autologous to the subject.
28. The method of claim 26 , wherein the adipose-derived SVF is allogeneic to the subject.
29. The method of claim 1 , wherein the composition containing the virus and cells was stored following culturing together and prior to use.