Therapeutic apoptotic cell preparations, method for producing same and uses thereof
The present application provides pharmaceutical compositions comprising a population of mononuclear-enriched cells in an early-apoptotic state, methods for the production of said compositions and uses thereof in the treatment of diseases characterized by pathological immune responses. The pharmaceutical compositions may be used in treatment of conditions such as, but not limited to, graft versus host disease (GVHD) and autoimmune diseases including but not limited to inflammatory bowel disease, gout and arthritis.
1. A method of treating, preventing, or ameliorating an immune disease, an autoimmune disease, or an inflammatory disease in a subject in need thereof, comprising administering to the subject a pharmaceutical composition comprising a stable, high-yield, early-apoptotic mononuclear-enriched cell population comprising at least 30% early apoptotic mononuclear-enriched cells, wherein said immune disease is selected from graft versus host disease (GVHD), high grade GVHD, acute GVHD, or any combination thereof, wherein said inflammatory disease is selected from gout, inflammatory bowel disease, Crohn's disease, and ulcerative colitis, or any combination thereof, wherein said early-apoptotic cell population is stable for more than 24 hours, and wherein production of said cell population comprises the following steps:
(a) freezing a mononuclear-enriched cell population in a freezing medium comprising an anticoagulant;
(b) thawing said mononuclear-enriched cell population; and
(c) inducing apoptosis in said thawed mononuclear-enriched cell population, said inducing comprising incubating said population in a medium comprising methylprednisolone and an anticoagulant.
2. The method of claim 1 , wherein the method induces a shift from high grade GVHD to grade I GVHD in said subject.
3. The method of claim 1 , wherein the method retains a graft-versus-tumor or graft-versus-leukemia (GVL) effect in said subject.
4. The method of claim 1 , wherein the method reduces hepatotoxicity associated with GVHD in said subject.
5. The method of claim 1 , wherein the subject is undergoing a hematopoietic stem-cell transplantation (HSCT) or a solid organ transplantation.
6. The method of claim 5 , wherein the HSCT is allogeneic HSCT, and said pharmaceutical composition comprises cells obtained from the same donor of the hematopoietic stem-cells.
7. The method of claim 5 , wherein the solid organ is selected from the group consisting of lung, heart, kidney, pancreas, liver, and small-bowel.
8. The method of claim 5 , wherein the administering of the pharmaceutical composition is carried out up to 24 hours prior to said transplantation, at the same time as same transplantation, or until 15 days following said transplantation.
9. The method of claim 1 , wherein said subject is suffering from a hematopoietic malignancy.
10. The method of claim 9 , wherein said hematopoietic malignancy is selected from the group consisting of leukemia, myelodysplastic syndrome (MDS), lymphoma, multiple myeloma, acute lymphoblastic leukemia (ALL), acute myeloid leukemia (AML), and chronic myelogenous leukemia (CML).
11. The method of claim 1 , wherein said pharmaceutical composition is administered by intravenous or local injection, or a combination thereof.
12. The method of claim 1 , wherein said pharmaceutical composition is administered at a dosage of about 120×10 6 −250×10 6 cells per kg body weight.
13. The method of claim 1 , wherein said mononuclear enriched cells are collected by leukapheresis.
14. The method of claim 1 , wherein said mononuclear enriched cells comprise at least one cell type selected from the group consisting of lymphocytes, monocytes, and natural killer cells.
15. The method of claim 1 , wherein said mononuclear enriched cells are obtained from a subject in need of receiving administration of a stable early apoptotic cell population, wherein said subject in need is selected from a subject suffering from a hematopoietic malignancy, a subject undergoing hematopoietic stem sell (HSCs) transplantation (HSCT), and a subject suffering from an inflammatory bowel disease (IBD).
16. The method of claim 1 , wherein said mononuclear enriched cells are obtained from a subject allogeneic with the subject in need of receiving administration of the stable early apoptotic cell population, wherein said subject in need is selected from a subject suffering from a hematopoietic malignancy, a subject undergoing hematopoietic stem sell (HSCs) transplantation (HSCT), and a subject suffering from an inflammatory bowel disease (IBD).
17. The method of claim 1 , wherein said composition further comprises an anti-coagulant.