Specific monoclonal antibody to acetylated mouse BubR1 and preparation method therefor
Provided is a rabbit monoclonal antibody to acetylated mouse BubR1 and a preparation method therefor and, more particularly, provides the use of the monoclonal antibody in a method for measuring the activity of cell division checkpoints on the basis of a degree of acetylation of BubR1, a method for detecting a tumor disease on the basis of aberrant cell division, a method for diagnosing cancer, a method for screening anti-cancer agents, or a method for regulating a cell division cycle.
1. A monoclonal antibody against acetylated mouse BubR1 produced by a hybridoma selected from the group consisting of mAcBubR1-SNU-20-4 (KCLRF-BP-00391), mAcBubR1-SNU-20-7 (KCLRF-BP-00392), and mAcBubR1-SNU-20-9 (KCLRF-BP-00393).
2. The monoclonal antibody of claim 1 , which is produced by mAcBubR1-SNU-20-7 (KCLRF-BP-00392).
3. The monoclonal antibody of claim 2 , which is specific to endogenous acetylated mouse BubR1.
4. The monoclonal antibody of claim 1 , which does not exhibit cross-reactivity to a mouse BubR1 protein having non-acetylated 243rd residue lysine and a mouse BubR1 protein having substitution of the 243rd residue lysine with arginine.
5. A hybridoma selected from the group consisting of mAcBubR1-SNU-20-4 (KCLRF-BP-00391), mAcBubR1-SNU-20-7 (KCLRF-BP-00392), and mAcBubR1-SNU-20-9 (KCLRF-BP-00393), which produces a monoclonal antibody against acetylated mouse BubR1.
6. A method of detecting acetylation degree of BubR1 protein, comprising determining the acetylation degree of the BubR1 protein using the monoclonal antibody of claim 1 .
7. The method of claim 6 , wherein the monoclonal antibody is produced by mAcBubR1-SNU-20-7 (KCLRF-BP-00392).
8. The method of claim 7 , wherein the acetylation degree is that of endogenous acetylated mouse BubR1.