IP Library › Granted Patent US 11,162,066
Granted Patent B2
US 11,162,066 · App. 16/071,533 · Granted Nov 2, 2021

Method for degrading microcystins in an aqueous medium

Inventors: Rehab El-Shehawy (Alcalá de Henares, ES); María Ángeles Lezcano Vega (Alcalá de Henares, ES); Jesús Morón López (Alcalá de Henares, ES)
Assignee: FUNDACIÓN IMDEA AGUA
C12N1/205C12N1/20C12R2001/01
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Quick Facts
Patent No.
US 11,162,066
App. No.
16/071,533
Granted
Nov 2, 2021
Kind
B2
Abstract

The invention relates to a bacterial strain capable of degrading microcystins in an aqueous medium. A method for reducing the content or for preventing the accumulation of microcystins in an aqueous medium is also disclosed. Finally, the present invention relates to an efficient method for isolating microcystin-degrading microorganisms.

Claims (11)

1. A method for preventing the accumulation or for reducing the content of microcystins present in an aqueous medium which comprises adding to said medium a microorganism from the species Sphingopyxis sp. deposited in the Spanish Type Culture Collection (CECT) with accession number CECT 8967 or a mutant thereof which substantially maintains the ability of degrading microcystins, or a biologically pure culture of said microrganism, a biomass comprising said microorganism, a bacterial consortium comprising said microorganism or an extract of said microorganism and maintaining the medium under conditions suitable for the degradation of said microcystins, thereby preventing the accumulation or reducing the content of said microcystins.

2. The method according to claim 1 wherein the microorganism or the biologically pure culture, the biomass, or the bacterial consortium has been expanded in a culture medium comprising at least one carbon source and at least one nitrogen source in appropriate conditions suitable for growth.

3. The method according to claim 1 or 2 wherein the microorganism, the biologically pure culture, the biomass, or the bacterial consortium has been maintained prior to the addition to the aqueous medium containing microcystins under nutrient starvation conditions.

4. The method according to claim 1 , wherein the concentration of organic carbon in the aqueous medium is from <1 to 300 mg/L.

5. The method according to claim 1 wherein said aqueous medium comprises microcystins in a concentration from 0.1 μg/L to 50,000 μg/L.

6. The method according to claim 1 , wherein said microcystins are selected from the group consisting of microcystin-RR, microcystin-LR, microcystin-YR and combinations thereof.

7. The method according to claim 1 , wherein the conditions suitable for degrading microcystins comprise:

temperature in range between 15° C. and 35° C.

aerobic conditions

pH between 6 and 8 and/or

incubation time between 1 and 120 hours.

Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Sep 6, 2018
From: EL-SHEHAWY, REHAB; LEZCANO VEGA, MARÍA ÁNGELES; LÓPEZ, JESÚS MORÓN
To: FUNDACIÓN IMDEA AGUA
Reel/Frame 046800/0493 →
Continuity (1)
Related Publication 20200080161A1 · Mar 12, 2020
Cited By (1)
US 12,365,604