ALK polypeptides and methods of use thereof
The invention features immunogenic compositions containing anaplastic lymphoma kinase (ALK) polypeptides and methods of use thereof. The immunogenic compositions and methods of the invention may be used to treat a disease associated with ALK in a subject, such as cancer (e.g., a solid tumor cancer or an ALK+ cancer).
1. An amphiphilic conjugate comprising:
(a) an albumin-binding domain;
(b) an ALK polypeptide; and
(c) an optional linker,
wherein the ALK polypeptide is 9 to 40 amino acids in length, comprises at least 6 contiguous amino acids from a sequence of SEQ ID NO: 3, and does not comprise a sequence of any one of SEQ ID NOs: 67-70 and 140-145, and
wherein the ALK polypeptide is conjugated directly to the albumin-binding domain or is conjugated to the albumin-binding domain through the linker.
2. The amphiphilic conjugate of claim 1 , wherein the albumin-binding domain is a lipid.
3. The amphiphilic conjugate of claim 1 , wherein the linker is selected from the group consisting of polymers, a string of amino acids, nucleic acids, polysaccharides, or a combination thereof.
4. An immunogenic composition comprising an amphiphilic conjugate of claim 1 .
5. A pharmaceutical composition comprising a therapeutically effective amount of an immunogenic composition of claim 4 and one or more pharmaceutically acceptable carriers or excipients.