Observation device
In the present invention, information is analyzed, the positional relationship of cells/microbes in the optical axis direction is detected, and motility of cells/microbes is evaluated even in an out-of-focus view from an image obtained by a single image capture in an observation view of the cells/microbes. The present invention is provided with an optical system used to measure microparticles present in a sample liquid in a sample container, a drive mechanism for driving the sample container and/or a portion of the optical system in order to three-dimensionally search a bottom surface of the sample container, a control unit for controlling the optical system or the drive mechanism, an image processing unit for dividing an image of microparticles in the sample container at a first time and a second time into an in-focus region and an out-of-focus region and acquiring information relating to the microparticles, and a display unit for displaying the information relating to the microparticles as information representing a temporal change between the first time and the second time.
1. An observation device comprising:
an optical system which is used to measure microparticles present in a sample liquid in a sample container;
a drive mechanism which drives at least any one of the sample container and a portion of the optical system to three-dimensionally search a bottom surface of the sample container;
a control unit which controls the optical system or the drive mechanism;
an image processing unit which divides an image of microparticles in the sample container at a first time and a second time into an in-focus region and an out-of-focus region, and acquires information relating to the microparticles; and
a display unit which displays the information relating to the microparticles as information representing a temporal change between the first time and the second time;
wherein the image processing unit performs division into the in-focus region and the out-of-focus region by performing an outline extraction using a contrast of the image;
wherein the information representing the temporal change is information representing a relation between information of an amount of microparticles floating in the sample liquid and a time, and the information on the amount of the microparticles floating in the sample liquid is calculated with a variation in luminance of an out-of-focus region as an index.
2. The observation device according to claim 1 , wherein
the out-of-focus region is further partitioned into a plurality of regions according to a defocus amount, and
an image processing is performed for each of the partitioned regions, and an amount of the microparticles is calculated for each of the partitioned regions to obtain a spatial distribution of the microparticles in the sample liquid.
3. The observation device according to claim 2 , wherein
a defocus correction is performed for each of the regions partitioned according to the defocus amount to obtain the spatial distribution of the microparticles in the sample liquid.
4. The observation device according to claim 2 , wherein
the display device displays information on the spatial distribution of the microparticles at the first time and information on the spatial distribution of the microparticles at the second time.
5. The observation device according to claim 1 ,
the information relating to the microparticles is at least one of the number of the microparticles, a size of the microparticles, a shape of the microparticles, and an occupation percentage of the microparticles on the focal plane.
6. The observation device according to claim 1 , wherein
the microparticles are microbes,
the observation device further includes a mechanism which performs temperature control on the sample liquid, and
the information representing the temporal change is information representing a relation between information on an amount of the microbes floating in the sample liquid and a time.
7. The observation device according to claim 6 , wherein
a data analyzing unit obtains information on a motility of the microbes from the information representing the temporal change.
8. The observation device according to claim 6 , wherein
a data analyzing unit obtains information on a response of a drug solution to the microbes from the information representing the temporal change before and after the drug solution is put in the sample liquid.