Method, biomarker and diagnostic agent for detection of high-risk prostate cancer
[Problem] To provide a method for detecting high-risk prostate cancer, for the purpose of providing useful information, such as necessity of biopsy, to a test-positive patient in a PSA test. [Solution] The method for detecting high-risk prostate cancer according to the present invention comprises reacting a PSA contained in a sample composed of urine collected from a human body which is suspected to be suffering from prostate cancer with (1) a fucose α1→6 affinitive lectin which has a characteristic property that the lectin has affinity expressed by a binding constant of 1.0×10 4 M −1 or more (at 25° C.) for an α1→6 fucose sugar chain No. 405. The fucose α1→6 affinitive lectin is preferably (2) a fucose α1→6 specific lectin which has a characteristic property that the lectin has a binding constant of 1.0×10 4 M −1 or less (at 25° C.) for a sugar chain No. 003 that does not contain α1→6 fucose and a glycolipid-type sugar chain No. 909 that does not contain α1→6 fucose.
1. A method for detecting high-risk prostate cancer, the method comprising:
reacting a PSA contained in a specimen composed of urine collected from a human body suspected of prostate cancer with a lectin capable of binding to an α1→6 fucose sugar chain;
comparing a reaction value of a signal attributed to the reaction of the PSA with the lectin with a reference value of a signal attributed to a reaction of a PSA contained in a specimen composed of urine collected from a person with Gleason score of 6 with the lectin;
evaluating a presence or an absence of the high-risk prostate cancer in the human body based on a result of the comparison between the reaction value and the reference value; and
detecting the presence of the high-risk prostate cancer in the human body based on the result of the comparison that indicates that the reaction value is lower than the reference value,
wherein the lectin is at least one selected from a group consisting of Aleuria aurantia lectin and Pholiota squarrosa lectin.
2. The method for detecting high-risk prostate cancer according to claim 1 , wherein
the specimen is urine of a person having a blood PSA level of 4 ng/mL or more.
3. The method for detecting high-risk prostate cancer according to claim 2 , wherein
the specimen is urine of a person having a blood PSA level of lower than 20 ng/mL.
4. The method for detecting high-risk prostate cancer according to claim 1 , wherein
the PSA is a free PSA (fPSA).
5. The method for detecting high-risk prostate cancer according to claim 1 , wherein the lectin is Pholiota squarrosa lectin.
6. The method for detecting high-risk prostate cancer according to claim 1 , wherein
the lectin is labeled.
7. The method for detecting high-risk prostate cancer according to claim 1 , wherein
the PSA is detected using the lectin, and one or more kinds of lectins or antibodies.
8. The method for detecting high-risk prostate cancer according to claim 7 , wherein the PSA is detected by an assay using the lectin and an anti-PSA antibody.
9. The method for detecting high-risk prostate cancer according to claim 8 , wherein the anti-PSA antibody is an anti-free PSA antibody (anti-fPSA antibody), and
the PSA is a free PSA (fPSA).