Implement for inspection, inspecting device and inspecting method
Provided is an implement for inspection capable of measuring the concentration of a test substance with high accuracy. The implement for inspection according to the present invention is an implement for inspection used for measuring the concentration of a test substance that includes a compound for reacting with a test substance to form a granular substance or a compound which is for being bound to the test substance and is a granular substance, and a wall portion having a periodic structure on its surface.
1. An implement for inspection used for measuring a concentration of a test substance, the implement for inspection comprising:
a compound for reacting with the test substance to form a granular substance or a compound which is for being bound to the test substance and is a granular substance; and
a wall portion having a periodic structure on a surface of the wall portion,
wherein the compound is not in contact with the periodic structure in the wall portion, and is disposed at a position distant from the periodic structure in the wall portion,
the granular substance formed by reacting the test substance with the compound or a granular substance in which the test substance is bound to the compound is deposited on the periodic structure, and
the periodic structure generates a peak of reflected light in the wavelength region of a photonic band gap when light is applied to the periodic structure.
2. The implement for inspection according to claim 1 , wherein
when the implement for inspection includes the compound for reacting with the test substance to form the granular substance, the compound for reacting with the test substance to form the granular substance is a compound for reacting with the test substance to form a granular substance in an aggregated state, and
when the implement for inspection includes the compound which is for being bound to the test substance and is the granular substance, the compound which is for being bound to the test substance and is the granular substance is bound to the test substance to form a granular substance in an aggregated state.
3. The implement for inspection according to claim 1 , comprising the compound which is for being bound to the test substance and is a granular substance, wherein
the test substance has an antigen, and
the compound has an antibody.
4. The implement for inspection according to claim 1 , comprising the compound for reacting with the test substance to form a granular substance, wherein
the test substance is glycolipid, and
the compound is an enzyme for reacting with glycolipid.
5. The implement for inspection according to claim 1 , comprising the compound for reacting with the test substance to form a granular substance, wherein
the test substance is endotoxin, and
the compound is LAL reagent.
6. An inspecting device comprising:
the implement for inspection according to claim 1 ;
a light source for applying light to the wall portion having the periodic structure of the implement for inspection; and
a measuring instrument for measuring intensity of light emitted from the light source and reflected by the wall portion having the periodic structure.
7. An inspecting method using the implement for inspection according to claim 1 , the inspecting method comprising:
a step of applying light to the periodic structure to measure reference light intensity in a state where the test substance is not introduced into the implement for inspection;
a step of applying light to the periodic structure to measure evaluation light intensity in a state where the test substance is introduced into the implement for inspection and the granular substance is deposited on the periodic structure; and
a step of acquiring a relative value of the reference light intensity and the evaluation light intensity, wherein
when the relative value obtained when a sample in which a concentration of the test substance is known is introduced into the implement for inspection is compared to the relative value obtained when a sample in which a concentration of the test substance is unknown is introduced into the implement for inspection, the concentration of the test substance in the sample in which the concentration of the test substance is unknown is determined.
8. The implement for inspection according to claim 1 , wherein the periodic structure of the wall portion has a plurality of recesses or a plurality of protrusions and the periodic structure of the wall portion is a periodic structure by the plurality of recesses or the plurality of protrusions.