Compositions for treatment of cancer, methods and systems for forming the same
Solutions having a solvent and a cold atmospheric plasma dissolved in the solvent are described. Methods and systems of forming cold atmospheric plasma (CAP)-containing solutions are also described. A system for producing (CAP)-containing solutions includes a gas source; a plasma generating device having a hollow body fluidically coupled with the gas source, a closed proximal end and an open distal end, the hollow body receiving gas from the gas source, and at least one electrode in or about the hollow body and ionizing the gas to discharge a cold atmospheric plasma (CAP) from the open distal end; and a container for housing a fluid, the open distal end of the plasma generating device in fluid communication with an inner portion of the container. CAP-containing solutions can be used in treatment of cancer cells, infected tissue sterilization, microorganism inactivation, promotion of wound healing, skin regeneration, and blood coagulation, and teeth bleaching/whitening.
1. A system for producing a cold atmospheric plasma (CAP)-containing solution, the system comprising:
a gas source;
a plasma generating device, the plasma generating device comprising:
a hollow body fluidically coupled with the gas source and having a closed proximal end and an open distal end, the hollow body receiving a gas from the gas source; and
at least one electrode in or about the hollow body and configured to ionize the gas to discharge a cold atmospheric plasma (CAP) from the open distal end, the at least one electrode comprising a first electrode extending longitudinally through at least a portion of the hollow body, and a second electrode extending around the hollow body in proximity to the distal end, wherein a high voltage power supply is electrically coupled with the first electrode and the second electrode; and
a container having an inner portion for housing a fluid, wherein the open distal end of the plasma generating device is configured to be submerged in the fluid such the CAP is discharged directly into the fluid.
2. The system of claim 1 , wherein the container further comprises an opening located at a bottom portion of the container, at least a portion of the plasma generating device protrudes into the container through the opening, and the opening and the plasma generating device are sealingly engaged.
3. The system of claim 1 , wherein the container further comprises an opening at a top portion of the container and the plasma generating device is in fluid communication with the inner portion of the container via the opening.
4. The system of claim 1 , wherein the gas source supplies one or more of nitrogen gas, helium gas, and argon gas to the plasma generating device.
5. The system of claim 1 , wherein the hollow body of the plasma generating device further comprises:
a main body,
a neck having a diameter smaller than the main body; and
a portion inwardly tapering from the main body to the neck,
wherein at least a portion of the main body defines the proximal end of the hollow body and the neck defines at least a portion of the open distal end of the hollow body.
6. The system of claim 5 , wherein the gas is ionized and the CAP is formed in the inwardly tapering portion.
7. The system of claim 5 , wherein the second electrode extends around an outer surface of the neck.
8. The system of claim 1 , further comprising a fluid housed in the container.
9. The system of claim 8 , wherein the fluid comprises deionized (DI) water, a water-based electrolyte solution, a phosphate-buffered saline (PBS) solution, a glucose solution, a cell culture medium, or any combination thereof.
10. The system of claim 8 , wherein the CAP is discharged directly into the fluid as any one of a bubble, jet or stream.
11. The system of claim 1 , wherein the gas source supplies helium gas to the plasma generating device.