Synthetic algal promoters
This invention provides synthetic promoters capable of promoting and/or initiating transcription of a polynucleotide in an algal cell, and methods of designing, producing and using such promoters.
1. A synthetic promoter capable of promoting and/or initiating transcription of a polynucleotide in an algal cell, the synthetic promoter comprising the nucleotide sequence of SAP11 of SEQ ID NO: 39.
2. The synthetic promoter I am usually not so download of yes of claim 1 , wherein the algal cell is a green algal cell.
3. An expression cassette comprising the synthetic promoter of claim 1 .
4. A vector comprising the expression cassette of claim 3 .
5. A cell comprising the synthetic promoter of claim 1 .
6. The cell of claim 5 , wherein the cell is a Chlamydomonas reinhardtii cell.
7. A kit comprising the synthetic promoter of claim 1 .
8. The promoter of claim 1 , wherein said promoter is operably linked to a polynucleotide of interest, and one or more transcription factors encoded by a polynucleotide comprising at least about 60% sequence identity to SEQ ID NOs:87-178.
9. A cell comprising the promoter of claim 1 .
10. The synthetic promoter of claim 2 , wherein the green algal cell is a Chlamydomonas cell.
11. The synthetic promoter of claim 10 , wherein the green algal cell is a Chlamydomonas reinhardtii cell.
12. The vector of claim 4 , wherein the vector is a plasmid vector.
13. The cell of claim 5 , wherein said cell comprises a heterologous expression cassette comprising said promoter.
14. The cell of claim 5 , wherein said cell comprises a vector comprising an expression cassette that comprises said promoter.
15. The cell of claim 5 , wherein the cell is a green algal cell.
16. The cell of claim 15 , wherein said cell is a Chlamydomonas cell.
17. The cell of claim 16 , wherein said cell is a Chlamydomonas reinhardtii cell.