SUBSTANCE LABELING PATCH, METHOD AND APPARATUS FOR TISSUE DIAGNOSIS USING THE SAME

1 .- 33 . (canceled)

34 . A tissue diagnosis method for detecting a target substance from a tissue sample by using a patch which includes a mesh structural body forming micro-cavities and is configured to contain a substance in the micro-cavities, the tissue diagnosis method comprising:

placing the tissue sample in a reaction region;

providing a fluorescence labeling substance for specifically labeling the target substance to the tissue sample by using a patch that contains the fluorescence labeling substance; and

detecting the fluorescence-labeled target substance from the tissue sample.

35 . The tissue diagnosis method of claim 34 , wherein the fluorescence labeling substance is a fluorescence labeling complex that includes a reaction derivative that reacts specifically with the target substance and a fluorescence marker for detecting the target substance.

36 . The tissue diagnosis method of claim 34 , wherein the detecting of the fluorescence-labeled target substance is performed by acquiring a fluorescence image of the tissue sample.

37 . The tissue diagnosis method of claim 34 , wherein:

the target substance is a target base sequence included in the tissue sample; and

the fluorescence labeling substance includes a fluorescence-labeled nucleic acid probe, the nucleic acid probe binding complementarily to the target base sequence.

38 . The tissue diagnosis method of claim 34 , wherein:

the target substance is a target protein included in the tissue sample; and

the fluorescence labeling substance include a fluorescence-labeled antibody, the antibody binds specifically to the target protein.

39 . The tissue diagnosis method of claim 34 , wherein the providing of the fluorescence labeling substance to the tissue sample includes:

contacting the patch containing the fluorescence labeling substance with the tissue sample; and

when the patch is in contact with the tissue sample, the fluorescence labeling substance is allowed to move to the reaction region.

40 . The tissue diagnosis method of claim 39 , wherein:

the providing of the fluorescence labeling substances to the tissue sample further includes separating the patch containing the fluorescence labeling substances from the tissue sample; and

when the patch is separated from the tissue sample, a residual fluorescence labeling substance that has not bound to the target substance of the fluorescence labeling substance is removed from the reaction region.

41 . The tissue diagnosis method of claim 34 , wherein the detecting of the fluorescence-labeled target substance is performed by measuring an amount of fluorescence emitted from the target substance included in the tissue sample.

42 . The tissue diagnosis method of claim 34 , wherein the detecting of the fluorescence-labeled target substance includes obtaining information on distribution of the target substance in the tissue sample.

43 . A substance labeling patch comprising:

a labeling substance that binds to a target substance included in a tissue sample to label the target substance; and

a mesh structural body having a mesh structure forming micro-cavities in which the labeling substance is contained that is configured to come into contact with the tissue sample and provide the labeling substance to a reaction region in which the target substance is placed.

44 . The substance labeling patch of claim 43 , wherein the labeling substance is a fluorescence labeling substance.

45 . The substance labeling patch of claim 44 , wherein:

the fluorescence labeling substance includes a fluorescence-labeled antibody; and

the target substance is a target protein included in the tissue sample.

46 . The substance labeling patch of claim 44 , wherein:

the fluorescence labeling substance includes a fluorescence-labeled nucleic acid probe; and

the target substance is a target base substance included in the tissue sample.

47 . The substance labeling patch of claim 43 , wherein the target substance is a DNA included in the tissue sample.

48 . The substance labeling patch of claim 43 , wherein the labeling substance is a color labeling substance.

49 . The substance labeling patch of claim 48 , wherein the color labeling substance includes an enzyme-attached antibody; and

the target substance is a target protein included in the sample.

50 . The substance labeling patch of claim 48 , wherein:

the color labeling substance includes hematoxylin; and

the target substance is a nucleus included in the sample.

51 . A tissue diagnosis device for detecting a target substance from a tissue sample by using a patch which includes a mesh structural body forming micro-cavities and is configured to contain a substance in the micro-cavities, the tissue diagnosis device comprising:

a plate supporter configured to support a plate on which a reaction region is placed and a sample is placed in the reaction region;

a patch controller configured to support the patch which contains a labeling substance that specifically labels the target substance, and control a position of the patch relative to the reaction region so that the patch comes into contact with the reaction region and provides the labeling substance to the reaction region; and

a target substance detector configured to detect the labeling substance and detect the target substance included in the tissue sample.

52 . The tissue diagnosis device of claim 51 , wherein the target substance detector include an imaging module configured to acquire an image of the reaction region in which the tissue sample is placed.

53 . The tissue diagnosis device of claim 51 , wherein the target substance detector includes a measurement module configured to measure an amount of the target substance included in the tissue sample.