Immunochromatographic device
The invention relates to an immunochromatographic device which contains a nitrous acid compound and an organic acid or an organic acid derivative and which is for detecting a detection target in an analyte wherein a sample droplet-receiving member, a labeling substance-holding member, a chromatography medium member and an absorption member are arranged in a manner that a sample develops in this order and wherein a part containing the nitrous acid compound and a part containing the organic acid or the like are at upstream positions from the labeling substance-containing part, and the part containing the nitrous acid compound and the part containing the organic acid or the like are not substantially in contact with each other in the thickness direction.
1. An immunochromatographic device for detecting a detection target in an analyte which comprises a sample droplet-receiving member, a labeling substance-holding member having a labeling substance-containing part, a chromatography medium member having a detection part and an absorption member and which contains a nitrous acid compound and an organic acid or an organic acid derivative,
wherein the sample droplet-receiving member, the labeling substance-holding member, the chromatography medium member and the absorption member are arranged in a manner that a sample develops in this order,
the immunochromatographic device has a part containing the nitrous acid compound and a part containing the organic acid or the organic acid derivative at upstream positions from the labeling substance-containing part, and
the part containing the nitrous acid compound and the part containing the organic acid or the organic acid derivative are not substantially in contact with each other in the thickness direction
wherein the organic acid or the organic acid derivative is a nitrogen-containing heterocyclic compound represented by the following formula (1),
wherein in the formula (1), R 1 and R 2 each independently represents a hydrogen atom, a carbonyl group or an alkyl group which may have a substituent, wherein alkyl groups may bind to each other to form a ring and form an alicyclic hydrocarbon ring or an aromatic hydrocarbon ring,
A represents a single bond or a double bond, X represents —C(═O)— or —(CH 2 ) n —, Y represents a carbon atom or a nitrogen atom, n is 1 or 2, and
Z represents a hydrogen atom, a hydroxyl group, an alkyl group which may have a substituent, —C(═O)R 3 or —O—C(═O)R 3 , wherein R 3 represents an alkyl group which may have a substituent or an alkoxy group.
2. The immunochromatographic device according to claim 1 , wherein the organic acid or the organic acid derivative is at least one selected from the group consisting of phthalimide, N-acetylphthalimide, N-(tert-butoxycarbonyloxy)phthalimide, N-hydroxysuccinimide, N-acetoxysuccinimide and hydantoin.
3. The immunochromatographic device according to claim 1 , wherein the nitrous acid compound is a nitrite.
4. The immunochromatographic device according to claim 1 , wherein the detection target is a Gram-positive bacterium.
5. The immunochromatographic device according to claim 4 , wherein the Gram-positive bacterium is a hemolytic Streptococcus.