Methods of preserving mesenchymal stem cells
View Patent ↗This disclosure relates to methods of preserving mesenchymal stromal/stem cells (MSCs) for use in clinical applications. In certain embodiments, this disclosure relates to methods of preserving MSCs comprising mixing MSCs with interferon-gamma prior to cryopreserving, freezing, or cooling the MSCs to a temperature below zero degrees Celsius.
1. A method of cryopreserving mesenchymal stem cells comprising
mixing mesenchymal stem cells with interferon gamma at a concentration of 20 ng/mL in culture medium for 48 hours providing mixed mesenchymal stem cells, and cooling the mixed mesenchymal stem cells to a temperature below zero degrees Celsius providing frozen interferon gamma exposed mesenchymal stem cells, wherein mixing is prior to cooling the mixed mesenchymal stem cells, and
thawing the frozen interferon gamma exposed mesenchymal stem cells to a temperature above zero degrees Celsius providing thawed interferon gamma exposed mesenchymal stem cells that express indoleamine 2,3-dioxygenase (IDO) mRNA with greater than a 100-fold change in concentration when compared to cryopreserved mesenchymal stem cells that were not mixed with interferon gamma prior to freezing.
2. The method of claim 1 , wherein the temperature is less than negative 50 degrees Celsius.
3. The method of claim 1 , wherein the mesenchymal stem cells are in a cryoprotectant during cooling.
4. The method of claim 3 wherein the cryoprotectant comprises a component selected from monosaccharide, a disaccharide, trehalose, glycerol, ethylene glycol, and propylene glycol, or combinations thereof.
5. The method of claim 3 wherein the cryoprotectant comprises a polyampholyte.
6. The method of claim 1 further comprising mixing the thawed interferon gamma exposed mesenchymal stem cells with a component selected from interferon-gamma, interleukin-1beta, interleukin-1alpha, transforming growth factor-beta, interleukin-6, interleukin-10, prostaglandin-E2, hepatocyte growth factor, tumor necrosis factor-alpha, or combinations thereof.
7. The method of claim 1 further comprising mixing the thawed interferon gamma exposed mesenchymal stem cells with interferon-gamma optionally in combination with a cytokine selected from interleukin-1beta, interleukin-1 alpha, and tumor necrosis factor-alpha.
8. The method of claim 1 further comprising purifying, plating, passaging, and harvesting the thawed mesenchymal stem cells providing regenerated mesenchymal stem cells.