Stabilized soluble pre-fusion RSV F proteins
Stable pre-fusion respiratory syncitial virus (RSV) F proteins (or fragment thereof) are described. Compositions containing the proteins and uses of the compositions for the prevention and/or treatment of RSV infection are also described.
1. A nucleic acid molecule encoding a recombinant pre-fusion respiratory syncytial virus (RSV) Fusion (F) protein, the nucleic acid molecule comprising a nucleotide sequence selected from the group consisting of SEQ ID NO: 20, SEQ ID NO: 21, and SEQ ID NO: 22.
2. The nucleic acid molecule according to claim 1 , wherein the nucleic acid molecule comprises the nucleotide sequence of SEQ ID NO: 20.
3. A vector comprising the nucleic acid molecule according to claim 2 .
4. A composition comprising the nucleic acid molecule according to claim 2 .
5. A method of inducing an immune response against RSV F protein in a subject in need thereof, the method comprising administering to the subject the composition according to claim 4 .
6. A method of prophylaxis and/or treatment of RSV infection in a subject in need thereof, the method comprising administering to the subject the composition according to claim 4 .
7. A vaccine against RSV comprising the nucleic acid molecule according to claim 2 .
8. The nucleic acid molecule according to claim 1 , wherein the nucleic acid molecule comprises the nucleotide sequence of SEQ ID NO: 21.
9. A vector comprising the nucleic acid molecule according to claim 8 .
10. A composition comprising the nucleic acid molecule according to claim 8 .
11. A method of inducing an immune response against RSV F protein in a subject in need thereof, the method comprising administering to the subject the composition according to claim 10 .
12. A method of prophylaxis and/or treatment of RSV infection in a subject in need thereof, the method comprising administering to the subject the composition according to claim 10 .
13. A vaccine against RSV comprising the nucleic acid molecule according to claim 8 .
14. The nucleic acid molecule according to claim 1 , wherein the nucleic acid molecule comprises the nucleotide sequence of SEQ ID NO: 22.
15. A vector comprising the nucleic acid molecule according to claim 14 .
16. A composition comprising the nucleic acid molecule according to claim 14 .
17. A method of inducing an immune response against RSV F protein in a subject in need thereof, the method comprising administering to the subject the composition according to claim 16 .
18. A method of prophylaxis and/or treatment of RSV infection in a subject in need thereof, the method comprising administering to the subject the composition according to claim 16 .
19. A vaccine against RSV comprising the nucleic acid molecule according to claim 14 .