Manufacture of a cereal-based lactic acid-fermented product
The present invention relates to a culture or kit-of-part comprising a Lactobacillus delbrueckii subsp lactis strain, and uses thereof to manufacture a cereal-based lactic acid-fermented product, in particular a maize-based lactic acid-fermented product such as mahewu.
1. A culture or kit-of-part, suitable for the manufacture of a ready-to-eat cereal-based lactic acid-fermented product, wherein the culture or kit-of-part comprises:
(a) a Lactobacillus delbrueckii subsp lactis strain which is a strong acidifier on cereal substrate when tested in assay A; and
(b) at least one other microorganism, wherein the at least one other microorganism is at least one Lactobacillus species strain(s) selected from the group consisting of Lactobacillus plantarum and Lactobacillus delbrueckii subsp bulgaricus , a proprionibacterium, or a protease-negative (prtS-) Streptococcus thermophilus strain; and
wherein the Lactobacillus delbrueckii subsp lactis strain and the at least one other microorganism are in frozen, dried, or freeze-dried form and are, each separately, at a concentration of at least 10 5 cfu per g of culture.
2. The culture or kit-of-part according to claim 1 , wherein:
said Lactobacillus delbrueckii subsp lactis strain is the Lactobacillus delbrueckii subsp lactis strain DGCC4550 deposited at the DSMZ under accession number DSM32049 on May 20, 2015 or a variant of said DSM32049 strain, and
said variant is a strong acidifier when tested in assay A.
3. The culture or kit-of-part according to claim 1 , wherein the Lactobacillus delbrueckii subsp lactis strain and the at least one other microorganism are, each separately, at a concentration in the range of 10 5 to 10 12 cfu per g of culture.