Sensors and sensor arrays for detection of analytes
Methods, apparatus and compositions are described for the measurement of metal and/or metalloid elements, including selenium in samples. More specifically, the invention provides a sensor and/or array of sensors to measure metal and/or metalloid analytes, e.g. sensor and/or array of sensors having a chelator molecule, the chelator molecule including a peptide which has a phenylalanine derivative.
1. A sensor or array of sensors comprising a chelator molecule, wherein the chelator molecule comprises a peptide, the peptide having a general formula of:
F 1 CZ 1 Z 2 CZ 3 Z 4 Z 5 CF 2 ,
wherein:
F 1 and F 2 are each independently a phenylalanine derivative of the structure:
wherein X is a halogen;
C is a cysteine amino acid; and
Z 1 , Z 2 , Z 3 , Z 4 , and Z 5 are each independently an amino acid.
2. The sensor or array of sensors of claim 1 , wherein F 1 is 4-iodophenylalanine (F4I).
3. The sensor or array of sensors of claim 1 , wherein F 2 is 4-bromophenylalanine (F4Br).
4. The sensor or array of sensors of claim 1 , wherein the peptide comprises one or more amino acid sequences of F4ICDICPNHCF4Br (SEQ ID NO: 1), F4ICQRCERWCF4Br (SEQ ID NO: 2), F4ICFHCFSECF4Br (SEQ ID NO: 3), F4ICAGCFTGCF4Br (SEQ ID NO: 4), and F4ICQLCNVLCF4Br (SEQ ID NO: 5),
wherein F4I is 4-iodophenylalanine and F4Br is 4-bromophenylalanine.
5. The sensor or array of sensors of claim 1 , wherein Z 1 , Z 2 , Z 3 , Z 4 , and Z 5 are each independently an amino acid selected from D, I, P, N, H, Q, R, E, W, S, A, G, F, T, L, V, or modifications thereof.