Formulation containing TLR agonist and methods of use
Stable aqueous formulations of adjuvant comprising a TLR7/8 agonist or a TLR4 agonist with a helper lipid that are adsorbed to alum are provided. Compositions and methods of using the formulations for stimulating an immune response are also provided.
1. A composition comprising:
(a) a TLR7/8 agonist, wherein the TLR7/8 agonist is 3M-052;
(b) a helper lipid, wherein the helper lipid is DSTAP, DLPG, DMPG, DPPG, DSPG, or DOPG, wherein a molar ratio of the TLR7/8 agonist to the helper lipid is about 1:2; and
(c) an aluminum salt, wherein the composition is an aqueous formulation comprising a stable nanosuspension of the TLR7/8 agonist and the helper lipid adsorbed to the aluminum salt.
2. The composition of claim 1 , wherein the stable nanosuspension has a particle size of 400 nm or less.
3. The composition of claim 1 , wherein the TLR7/8 agonist in the stable nanosuspension is adsorbed to the aluminum salt at 75 percent or more of the aluminum salt.
4. The composition of claim 1 , wherein the aluminum salt is selected from the group consisting of aluminum hydroxide, aluminum trihydrate, aluminum oxyhydroxide, aluminum phosphate, aluminum hydroxyphosphate, aluminum hydroxyphosphate sulfate, and potassium aluminum sulfate.
5. The composition of claim 4 , wherein the aluminum salt comprises an aluminum hydroxide gel.
6. The composition of claim 4 , wherein the aluminum salt comprises an aluminum phosphate gel.
7. A composition comprising:
(a) a TLR7/8 agonist, wherein the TLR7/8 agonist is 3M-052;
(b) a helper lipid; and
(c) an aluminum salt, wherein the aluminum salt is aluminum hydroxide gel and the helper lipid is DLPG, DMPG, DPPG, DSPG, or DOPG, wherein the composition is an aqueous formulation comprising a stable nanosuspension of the TLR7/8 agonist and the helper lipid adsorbed to the aluminum salt.
8. A composition comprising:
(a) a TLR7/8 agonist, wherein the TLR7/8 agonist is 3M-052;
(b) a helper lipid; and
(c) an aluminum salt, wherein the aluminum salt is aluminum phosphate gel and the helper lipid is DSTAP, wherein the composition is an aqueous formulation comprising a stable nanosuspension of the TLR7/8 agonist and the helper lipid adsorbed to the aluminum salt.
9. The composition of claim 1 , further comprising an antigen.
10. The composition of claim 9 , wherein the antigen is selected from a tuberculosis-related antigen, influenza-related antigen, hemagglutinin-related antigen, cancer-related antigen, viral-related antigen and amebiasis-related antigen.
11. The composition of claim 10 , wherein the tuberculosis-related antigen is selected from the group consisting of ID93, 1D91, and BCG.
12. The composition of claim 10 , wherein the influenza-related antigen is selected from the group consisting of H5N1, influenza A, influenza B, and influenza C.
13. The composition of claim 10 , wherein the amebiasis-related antigen is LecA.
14. The composition of claim 10 , wherein the viral-related antigen is selected from the group consisting of hepatitis B and hepatitis C.
15. The composition of claim 1 , wherein the composition is stable at 2-8° C. for at least 16 weeks.
16. The composition of claim 1 , wherein the composition is a vaccine.
17. The composition of claim 2 , wherein the stable nanosuspension has a particle size of 200 nm or less.