Enzymatically polymerized gelling dextrans
Compositions are disclosed herein comprising dextran that comprises (i) 87-93 wt % glucose linked at positions 1 and 6; (ii) 0.1-1.2 wt % glucose linked at positions 1 and 3; (iii) 0.1-0.7 wt % glucose linked at positions 1 and 4; (iv) 7.7-8.6 wt % glucose linked at positions 1, 3 and 6; and (v) about 0.4-1.7 wt % glucose linked at (a) positions 1, 2 and 6, or (b) positions 1, 4 and 6. Aqueous forms of this composition have enhanced viscosity profiles. Further disclosed are methods of using compositions comprising dextran, such as increasing the viscosity of an aqueous composition. Enzymatic reactions for producing dextran are also disclosed.
1. An isolated composition comprising water, sucrose and a glucosyltransferase enzyme comprising an amino acid sequence that is at least 98% identical to SEQ ID NO:1 or SEQ ID NO:2,
wherein the isolated composition is a food product selected from a cream soup, gravy, sauce, salad dressing, mayonnaise, frozen dessert, ice cream, or cultured dairy product,
and wherein the food product comprises dextran synthesized by the glucosyltransferase enzyme.
2. The isolated composition of claim 1 , wherein the glucosyltransferase enzyme comprises an amino acid sequence that is at least 99% identical to SEQ ID NO:1 or SEQ ID NO:2.
3. The isolated composition of claim 1 , wherein the glucosyltransferase enzyme comprises an amino acid sequence that is 100% identical to SEQ ID NO:1.
4. The isolated composition of claim 1 , wherein the glucosyltransferase enzyme comprises an amino acid sequence that is 100% identical to SEQ ID NO:2.
5. The isolated composition of claim 1 , comprised within an inert vessel.
6. The isolated composition of claim 5 , comprised within a stainless steel vessel, plastic vessel, or glass vessel.
7. The isolated composition of claim 1 , wherein the isolated composition comprises only one type of glucosyltransferase enzyme.
8. The isolated composition of claim 1 , wherein the food product is the cultured dairy product.
9. The isolated composition of claim 8 , wherein the cultured dairy product is cottage cheese or cheese.
10. The isolated composition of claim 8 , wherein the cultured dairy product is sour cream.
11. The isolated composition of claim 8 , wherein the cultured dairy product is yogurt.
12. The isolated composition of claim 1 , wherein the isolated composition is cell-free.