Esculentin 1a derivatives and uses thereof
View Patent ↗Provided herein are synthetic antibacterial peptides that have a sequence at least 80% identical to a sequence shown in SEQ ID NO: 2 or the diastereomer thereof with a sequence shown in SEQ ID NO: 3 or pharmaceutical compositions thereof. Also provided are methods for stimulating wound healing via the synthetic antibacterial peptides and for forming an antimicrobial coating on contact lenses. In addition a device is provided that has a surface with a coating of the synthetic antibacterial peptides.
1. An antimicrobial lens formed by coating a contact lens with an antimicrobial component comprising an antibacterial peptide with a sequence at least 80% identical to the sequence of SEQ ID NO: 2 or a diastereomer thereof with a sequence at least 80% identical to the sequence of SEQ ID NO: 3, said coating incorporated onto the lens or into the lens or a combination thereof, wherein said coating comprises about 2 μg of the antibacterial peptide.
2. The antimicrobial lens of claim 1 , wherein the antimicrobial component is effective to prevent or treat an ocular surface infection caused by Gram-positive bacteria or Gram-negative bacteria.
3. The antimicrobial lens of claim 2 , wherein the ocular surface infection is microbial keratitis.
4. A contact lens with at least one surface comprising a coating of an effective amount of an antibacterial peptide, wherein the coating comprises about 2 μg of the antibacterial peptide, and wherein the antibacterial peptide comprises a sequence at least 80% identical to the sequence of SEQ ID NO: 2 or a diastereomer thereof with a sequence at least 80% identical to the sequence of SEQ ID NO: 3.
5. The contact lens of claim 4 , wherein the surface further comprises a polymer hydrogel, a silicone hydrogel, 2-hydroxyethylmethacrylate polymer or a copolymer or mixtures thereof.
6. The antimicrobial lens of claim 1 , wherein coating the contact lens comprises:
treating the contact lens with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC);
exposing the treated contact lens to a solution containing the antimicrobial component under conditions sufficient to incorporate an effective amount of the antimicrobial component on and/or into the lens; and
immersing said treated contact lens in a solution of sodium chloride to obtain a layer of the antibacterial component coated onto the contact lens.
7. The antimicrobial lens of claim 6 , wherein the sodium chloride is about 10% wt/vol in the solution.
8. The antimicrobial lens of claim 6 , wherein the conditions comprise a temperature of about 25° C. for a time of about 15 minutes to about 37° C. for a time of about 2 hours.
9. The antimicrobial lens of claim 1 , wherein the antimicrobial component comprises an antibacterial peptide with a sequence at least 80% identical to the sequence of SEQ ID NO:3.
10. The contact lens of claim 4 , wherein the antibacterial peptide comprises a sequence at least 80% identical to the sequence of SEQ ID NO:3.