Promoter useful for high expression of a heterologous gene of interest in
The present invention provides for an Aspergillus niger host cell comprising a gene of interest operatively linked to an ecm33 promoter of an ascomycete fungi, wherein the gene of interest is heterologous to the ecm33 promoter and/or to Aspergillus niger . In some embodiments, the gene of interest is a glycoside hydrolase enzyme. In some embodiments, the glycoside hydrolase enzyme is a glucosidase.
1. An Aspergillus niger host cell comprising a nucleic acid encoding a gene of interest operatively linked to an ecm33 promoter of an ascomycete fungi, wherein the gene of interest is heterologous to the ecm33 promoter and/or to the Aspergillus niger host cell.
2. The Aspergillus niger cell of claim 1 , wherein the ascomycete fungi is an Aspergillus species.
3. The Aspergillus niger cell of claim 2 , wherein the Aspergillus species is an Aspergillus niger.
4. The Aspergillus niger host cell of claim 3 , wherein the Aspergillus niger is an Aspergillus niger strain 1015.
5. The Aspergillus niger host cell of claim 1 , wherein the ecm33 promoter comprises the nucleotide sequence of SEQ ID NO:1.
6. The Aspergillus niger host cell of claim 1 , wherein the gene of interest is a glycoside hydrolase enzyme.
7. The Aspergillus niger host cell of claim 6 , wherein the glycoside hydrolase enzyme is a glucosidase.
8. The Aspergillus niger host cell of claim 1 , wherein the ecm33 promoter is heterologous to Aspergillus niger host cell.
9. The Aspergillus niger host cell of claim 8 , wherein the gene of interest is heterologous to Aspergillus niger host cell.
10. The Aspergillus niger host cell of claim 1 , wherein the gene of interest is heterologous to Aspergillus niger host cell.
11. A method of expressing a heterologous gene of interest in an Aspergillus niger host cell, comprising: (a) introducing a nucleic acid encoding a queen of interest operatively linked to an ecm33 promoter of an ascomycete fungi, wherein the queen of interest is heterologous to the ecm33 promoter and/or to Aspergillus niger , into an A. niger host cell, and (b) culturing or growing the host cell in a medium for expressing the gene of interest.
12. The method of claim 11 , further comprising (c) separating or purifying a gene product encoded by the gene of interest from the host cell and/or medium.