Molecular analysis system and use thereof
A molecular testing device comprises a heating and cooling module having a thin-film thermoelectric heating and cooling device, and a removable test module having a combined amplification and hybridization reaction chamber. The reaction chamber comprises a thermo-conductive exterior surface and a microarray on an interior surface. The thin-film thermoelectric heating and cooling device has a heat transfer surface that is adapted to make contact with the thermo-conductive exterior surface of the reaction chamber. The molecular testing device may be used to perform a PCR in the reaction chamber.
1. A method for performing a polymerase chain reaction (PCR), comprising the step of:
placing a test module in a PCR device; and
heating and cooling the test module based on a predetermined PCR program stored in the PCR device,
wherein the test module comprises a reaction chamber having a thermo-conductive first exterior surface, a second exterior surface and a microarray on an interior surface, wherein the microarray is an ordered array of spots presented for binding to ligands of interest, and
wherein the PCR device comprises:
a heating and cooling module comprising a thermoelectric heating and cooling device comprising a heat transfer surface;
a sample holder for receiving the removable test module, wherein the thermoelectric heating and cooling device heats and cools depending on the direction of an electrical current the reaction chamber by contacting the heat transfer surface with the thermo-conductive first exterior surface of the reaction chamber, and wherein the sample holder comprises a thermal insulation layer to insulate the second exterior surface of the reaction chamber;
a moving system that brings the heat transfer surface in contact with the thermo-conductive first exterior surface when the test module is placed in the sample holder, wherein the heat transfer surface undergoes thermal cycling during the PCR; and
a programmable control module configured to control the direction of an electrical current flowing through the thermoelectric heating and cooling device to control the heating and cooling of the thermoelectric heating and cooling device.
2. The method of claim 1 , wherein the thermoelectric device is a Peltier device.
3. The method of claim 1 , wherein the thermoelectric heating and cooling device comprises a semiconductor and a heat sink.
4. The method of claim 1 , wherein the heating and cooling module further comprises a temperature sensor.
5. The method of claim 4 , wherein the temperature sensor comprises a thermistor or resistance thermal device.
6. The method of claim 1 , wherein the heating and cooling module comprises a plurality of thermoelectric heating and cooling devices each comprising a heat transfer surface, wherein the test module comprises a plurality of reaction chambers each having a thermo-conductive first exterior surface, wherein said programmable control module is capable of regulating temperature of each of said heat transfer surface individually in order to perform PCR under different conditions in each reaction chamber.