HER2 binders
Imaging agents comprising an isolated polypeptide conjugated with a radionuclide and a chelator; wherein the isolated polypeptide binds specifically to HER2, or a variant thereof; and methods for preparing and using these imaging agents.
1. A method of imaging at least a portion of a subject comprising:
administering a composition to the subject, wherein the composition comprises an isolated polypeptide and an 18 F coupled to the peptide through a bifunctional linker molecule, wherein the 18 F is coupled to the bifunctional linker via an aminoxy group,
wherein the isolated polypeptide comprises SEQ. ID No. 1 or SEQ. ID No. 2, and
wherein the isolated polypeptide binds specifically to HER2; and
imaging the subject with PET.
2. The method of claim 1 , further comprising the step of diagnosing the subject with a HER2 expressing cancer.
3. The method of claim 2 , wherein the HER2 expressing cancer is breast cancer.
4. The method of claim 1 , wherein the bifunctional aminoxy linker is a tert-butyl 2-(2-(2,5-dioxo-2,5-dihydro-1H-pyrrol-1-yl)ethylamino)-2-oxoethoxycarbamate linker.
5. The method of claim 1 , wherein the ′ 8 F is conjugated to the isolated polypeptide via the bifunctional aminoxy linker at the N-terminus of the isolated polypeptide.
6. The method of claim 1 , further comprising the step of monitoring delivery of the composition to the subject.
7. A method of imaging at least a portion of a subject comprising:
administering a composition to the subject, wherein the composition comprises an isolated polypeptide comprising SEQ. ID No. 2 conjugated with a 18 F via a tert-butyl 2-(2-(2,5-dioxo-2,5-dihydro-1H-pyrrol-1-yl)ethylamino)-2-oxoethoxycarbamate linker, wherein the isolated polypeptide binds specifically to HER2; and
imaging the subject with PET.
8. The method of claim 7 , further comprising the step of diagnosing the subject with a HER2 expressing cancer.
9. The method of claim 8 , wherein the HER2 expressing cancer is breast cancer.
10. The method of claim 7 , further comprising the step of monitoring delivery of the composition to the subject.