Diagnostic, prognostic, and monitoring methods for chronic lymphocytic leukemia
The invention generally provides improved compositions and methods for detecting, diagnosing, prognosing, and monitoring multiple myeloma, chronic lymphocytic leukemia, or B-cell non-Hodgkin lymphoma in a subject. In particular, the invention provides methods for detecting BCMA in subjects to reliably diagnose, predict survival, or monitor multiple myeloma, chronic lymphocytic leukemia, or B-cell non-Hodgkin lymphoma in the subject.
1. A method of assaying for BCMA, comprising:
(a) providing a first biological sample obtained from a patient diagnosed with chronic lymphocytic leukemia (CLL) and a second biological sample from the patient diagnosed with CLL,
(b) detecting a first amount of BCMA polypeptide or fragment thereof in the first biological sample, and
(c) detecting a second amount of BCMA polypeptide or fragment thereof in the second biological sample;
wherein the first biological sample and the second biological sample are each independently:
(i) a serum sample obtained from the patient's blood,
(ii) a plasma sample obtained from the patient's blood,
(iii) a supernatant sample obtained from culture of the patient's bone marrow mononuclear cells, or
(iv) a supernatant sample obtained from culture of the patient's peripheral blood mononuclear cells.
2. The method of assaying for BCMA according to claim 1 , wherein the first biological sample is a first serum sample obtained from the subject's blood, the first serum sample being obtained prior to administration of a treatment for CLL, and wherein the second biological sample is a second serum sample obtained from the subject's blood, the second serum sample being obtained after administration of a treatment for CLL.
3. The method of assaying for BCMA according to claim 1 , wherein the first biological sample is a first plasma sample obtained from the subject's blood, the first plasma sample being obtained prior to administration of a treatment for CLL, and wherein the second biological sample is a second plasma sample obtained from the subject's blood, the second plasma sample being obtained after administration of a treatment for CLL.
4. The method of assaying for BCMA according to claim 1 , wherein the first biological sample is a first supernatant sample obtained from culture of the patient's bone marrow mononuclear cells, the first biological sample being obtained prior to administration of a treatment for CLL to the patient, and
wherein the second biological sample is a second supernatant sample obtained from culture of the patient's bone marrow mononuclear cells, the second biological sample being obtained after to administration of a treatment for CLL to the patient.
5. The method of assaying for BCMA according to claim 1 , wherein the first biological sample is a first supernatant sample obtained from culture of the patient's peripheral blood mononuclear cells, the first biological sample being obtained prior to administration of a treatment for CLL to the patient, and
wherein the second biological sample is a second supernatant sample obtained from culture of the patient's peripheral blood mononuclear cells, the second biological sample being obtained after to administration of a treatment for CLL to the patient.
6. The method of assaying for BCMA according to claim 1 , wherein the detecting steps are performed using enzyme-linked immunosorbent assay (ELISA).
7. The method of assaying for BCMA according to claim 1 , wherein the detecting steps are performed using radioimmunoassay (RIA).
8. The method of assaying for BCMA according to claim 1 , wherein the detecting steps are performed using enzyme immunoassay (EIA).
9. The method of assaying for BCMA according to claim 1 , wherein the detecting steps are performed using fluorescence immunoassay (FIA).
10. The method of assaying for BCMA according to claim 1 , wherein the detecting steps are performed using luminescence immunoassay (LIA).
11. The method of assaying for BCMA according to claim 1 , wherein the detecting steps are performed using lateral flow assay.
12. The method of assaying for BCMA according to claim 1 , wherein the detecting steps are performed using strip assay.
13. The method of assaying for BCMA according to claim 2 , wherein the second amount being greater than the first amount indicates that said CLL is progressing, and wherein the first amount being greater than the second amount indicates that said CLL is entering remission or responding to treatment.
14. The method of assaying for BCMA according to claim 3 , wherein the second amount being greater than the first amount indicates that said CLL is progressing, and wherein the first amount being greater than the second amount indicates that said CLL is entering remission or responding to treatment.
15. The method of assaying for BCMA according to claim 4 , wherein the second amount being greater than the first amount indicates that said CLL is progressing, and wherein the first amount being greater than the second amount indicates that said CLL is entering remission or responding to treatment.
16. The method of assaying for BCMA according to claim 5 , wherein the second amount being greater than the first amount indicates that said CLL is progressing, and wherein the first amount being greater than the second amount indicates that said CLL is entering remission or responding to treatment.
17. A method of determining progression of chronic lymphocytic leukemia (CLL), comprising:
(a) providing a first biological sample obtained from a patient diagnosed with CLL and a second biological sample from the patient diagnosed with CLL,
(b) detecting a first amount of BCMA polypeptide or fragment thereof in the first biological sample, and
(c) detecting a second amount of BCMA polypeptide or fragment thereof in the second biological sample;
wherein the first biological sample and the second biological sample are each independently:
(i) a serum sample obtained from the patient's blood, or
(ii) a plasma sample obtained from the patient's blood;
further wherein the first biological sample is obtained prior to administration of a treatment for CLL and the second biological sample is obtained after administration of the treatment for CLL; and
wherein the second amount being greater than the first amount indicates that said CLL is progressing, and wherein the first amount being greater than the second amount indicates that said CLL is entering remission or responding to treatment.