Sample dishes for use in microscopy and methods of their use
Disclosed herein are sample dishes for use with microscopes that are simple to mount on a microscope and facilitate easy manipulation of tissue samples disposed thereon during imaging as well as methods of their use. A sample dish comprises an optical interface and, optionally, a support member that holds the optical interface. The optical interface of a sample dish is suitably transparent and planar such that a focal plane of a microscope can reside uniformly at or within a surface of a sample during imaging. In certain embodiments, a support member comprises a dish for holding excess fluid. In certain embodiments, a sample dish comprises separation ribs. In certain embodiments, a sample dish comprises one or more manipulation members (e.g., tabs). In certain embodiments, a sample dish is used with an imaging artifact reducing fluid.
1. A sample dish for use in imaging a sample, the dish comprising:
a support member; and
a substantially planar transparent optical interface having a sample side and a mounting side opposite the sample side, wherein the optical interface is attached to the support member,
wherein the support member comprises a ditch that surrounds a periphery of the optical interface, the ditch comprises an outer lip, and the optical interface is disposed such that a surface of the optical interface on the sample side is entirely above the outer lip.
2. The sample dish of claim 1 , wherein the sample dish is a single use dish.
3. The sample dish of claim 1 , wherein the sample dish is sterile.
4. The sample dish of claim 1 , wherein the sample side is exposed to a user when the sample dish is mounted on a microscope.
5. The sample dish of claim 1 , comprising one or more manipulation elements that protrude from the ditch of the support member above the outer lip.
6. The sample dish of claim 5 , wherein the one or more manipulation elements are two tabs sized and shaped to be grasped by a user's hands, wherein the two tabs are disposed around a periphery of the dish.
7. The sample dish of claim 5 , wherein each of the one or more manipulation elements is a portion of the support member.
8. The sample dish of claim 5 , wherein the one or more manipulation elements each protrude upwardly and outwardly from the outer lip above the optical interface.
9. The sample dish of claim 5 , wherein the support member is a one-part component.
10. The sample dish of claim 1 , wherein the optical interface has a circular cross section.
11. The sample dish of claim 10 , wherein the optical interface has a diameter of at least 50 mm.
12. The sample dish of claim 1 , wherein the optical interface comprises at least one of imaging grade plastic, sapphire, and glass.
13. The sample dish of claim 12 , wherein the optical interface consists essentially of imaging grade plastic.
14. The sample dish of claim 1 , wherein the optical interface has a thickness between 5 μm and 500 μm.
15. The sample dish of claim 14 , wherein the optical interface has a thickness of about 50 μm.
16. The sample dish of claim 1 , wherein the optical interface is sized and shaped such that only the optical interface of the sample dish contacts a microscope when the sample dish is mounted on the microscope.
17. The sample dish of claim 1 , wherein the optical interface is flexible.
18. The sample dish of claim 1 , wherein the ditch is at least 3 mm deep.
19. The sample dish of claim 1 , wherein the ditch is sized and shaped to contain at least 5 mL of fluid.
20. The sample dish of claim 1 , wherein the support member comprises a plurality of separation ribs disposed at least partially within the ditch such that the plurality of separation ribs partition the ditch.
21. The sample dish of claim 1 , wherein the ditch comprises an inner lip and the optical interface is disposed on and above the inner lip.
22. The sample dish of claim 1 , wherein the optical interface has a relative mass variation of no more than 0.1% as measured using a ASTM International D570 test.
23. The sample dish of claim 1 , wherein an anti-reflection coating is disposed on the mounting side of the optical interface.
24. A set of sample dishes comprising a plurality of sample dishes according to claim 1 , wherein, for each of the plurality of sample dishes, a thickness of the optical interface is within 5 μm of a standardized thickness for the set.
25. The set of sample dishes of claim 24 , wherein the plurality of sample dishes comprises at least 20.
26. The sample dish of claim 1 , wherein the support member has a surface roughness (Ra) of no more than 6 micrometers.
27. The sample dish of claim 1 , wherein the support member comprises an outwardly sloped inner lip.
28. The sample dish of claim 1 , wherein the optical interface is entirely above the outer lip.
29. The sample dish of claim 1 , wherein the sample dish is mounted on an imaging system, the sample is disposed on the optical interface on the sample side, and the sample is accessible to a user during the imaging, wherein light is transmitted through the optical interface during the imaging.
30. A sample dish for use in imaging a sample, the dish comprising:
a flexible transparent optical interface comprising imaging grade plastic, the optical interface having a sample side and a mounting side opposite the sample side; and
a support member,
wherein the optical interface is attached to the support member and the optical interface is disposed such that a surface of the optical interface on the sample side is entirely above the support member.
31. The sample dish of claim 30 , wherein the optical interface is disposed entirely above the support member.