Immunotherapy against melanoma and other cancers
A method of treating a patient who has melanoma includes administering to said patient a composition containing a population of activated T cells that selectively recognize cells in the patient that aberrantly express a peptide. A pharmaceutical composition contains activated T cells that selectively recognize cells in a patient that aberrantly express a peptide, and a pharmaceutically acceptable carrier, in which the T cells bind to the peptide in a complex with an MHC class I molecule, and the composition is for treating the patient who has melanoma. A method of treating a patient who has melanoma includes administering to said patient a composition comprising a peptide in the form of a pharmaceutically acceptable salt, thereby inducing a T-cell response to the melanoma.
1. A method for treating a patient who has cancer comprising administering to the patient a population of activated T cells that selectively recognize cancer cells that present a peptide consisting of the amino acid sequence of FVYGEPREL (SEQ ID NO: 45),
wherein said cancer is selected from the group consisting of melanoma, acute myelogenous leukemia, bile duct cancer, brain cancer, gallbladder cancer, gastric cancer, hepatocellular cancer, non-small cell lung cancer, ovarian cancer, pancreatic cancer, prostate cancer, renal cell cancer, small cell lung cancer, urinary bladder cancer, and uterine cancer.
2. The method of claim 1 , wherein the T cells are autologous to the patient.
3. The method of claim 1 , wherein the T cells are obtained from a healthy donor.
4. The method of claim 1 , wherein the activated T cells are produced by contacting T cells with the peptide loaded human class I or II MHC molecules expressed on the surface of an antigen-presenting cell for a period of time sufficient to activate the T cells.
5. The method of claim 1 , wherein the activated T cells are expanded in vitro.
6. The method of claim 1 , wherein the peptide is in a complex with an MHC class I molecule.
7. The method of claim 4 , wherein the antigen presenting cell is infected with a recombinant virus expressing the peptide.
8. The method of claim 7 , wherein the antigen presenting cell is a dendritic cell or a macrophage.
9. The method of claim 5 , wherein the expansion is in the presence of an anti-CD28 antibody and IL-12.
10. The method of claim 1 , wherein the population of activated T cells comprises CD8-positive cells.
11. The method of claim 4 , wherein the contacting is in vitro.
12. The method of claim 1 , wherein the population of activated T cells are administered in the form of a composition.
13. The method of claim 12 , wherein the composition comprises an adjuvant.
14. The method of claim 13 , wherein the adjuvant is selected from the group consisting of anti-CD40 antibody, imiquimod, resiquimod, GM-CSF, cyclophosphamide, Sunitinib, bevacizumab, interferon-alpha, interferon-beta, CpG oligonucleotides and derivatives, poly-(I:C) and derivatives, RNA, sildenafil, particulate formulations with poly(lactide co-glycolide) (PLG), virosomes, interleukin (IL)-1, IL-2, IL-4, IL-7, IL-12, IL-13, IL-15, IL-21, and IL-23.
15. The method of claim 1 , wherein the cancer is melanoma.
16. A method of eliciting an immune response in a patient who has cancer comprising administering to the patient a population of activated T cells that selectively recognize cancer cells that present a peptide consisting of the amino acid sequence of FVYGEPREL (SEQ ID NO: 45),
wherein said cancer is selected from the group consisting of melanoma, acute myelogenous leukemia, bile duct cancer, brain cancer, gallbladder cancer, gastric cancer, hepatocellular cancer, non-small cell lung cancer, ovarian cancer, pancreatic cancer, prostate cancer, renal cell cancer, small cell lung cancer, urinary bladder cancer, and uterine cancer.
17. The method of claim 16 , wherein the activated T cells are produced by contacting T cells with the peptide loaded human class I or II MHC molecules expressed on the surface of an antigen-presenting cell for a period of time sufficient to activate the T cells.
18. The method of claim 16 , wherein the immune response comprises a cytotoxic T cell response.
19. The method of claim 16 , wherein the immune response is capable of killing cancer cells that present a peptide consisting of the amino acid sequence of FVYGEPREL (SEQ ID NO: 45).
20. The method of claim 16 , wherein the cancer is melanoma.