Adipose tissue products and methods of production
Compositions and methods for treating tissue are provided. The compositions may include tissue matrix derived from adipose tissue suitable for injection, small-volume implantation, or use as a soft-tissue regenerative material. Also provided are methods for producing such compositions.
1. A method for producing a tissue product, comprising:
selecting an adipose tissue;
mechanically processing the adipose tissue to reduce the tissue size;
treating the mechanically processed tissue to remove substantially all cellular material from the tissue;
suspending the tissue in a solution to form a suspension;
treating the suspension to produce a stabilized three-dimensional structure with a micro-porous structure; and
mechanically processing the stabilized three-dimensional structure to produce particles.
2. The method of claim 1 , wherein treating the suspension to produce a stabilized three-dimensional structure includes drying the suspension.
3. The method of claim 2 , wherein drying includes freeze-drying.
4. The method of claim 1 , wherein treating the suspension to produce a stabilized three-dimensional structure includes cross-linking the tissue.
5. The method of claim 4 , wherein cross-linking includes treatment with at least one of a chemical, a photo-activated cross-linking process, or heating.
6. The method of claim 5 , wherein the chemical includes at least one of glutaraldehyde, genepin, carbodiimides, and diisocyantes.
7. The method of claim 4 , wherein cross-linking includes heating the tissue.
8. The method of claim 7 , wherein the tissue is heated in a vacuum.
9. The method of claim 4 , wherein the tissue is heated to 70° C. to 120° C.
10. The method of claim 1 , wherein the stabilized three-dimensional structure maintains its micro-porous structure when in contact with an aqueous environment.
11. The method of claim 10 , wherein the material maintains the stable three-dimensional structure when implanted in a body.
12. The method of claim 1 , wherein mechanically processing the stabilized three-dimensional structure to produce particles includes producing a group of particles comprising a longest dimension between 50 microns to 4 mm.
13. The method of claim 1 , wherein mechanically processing the stabilized three-dimensional structure to produce particles includes producing a group of particles comprising a longest dimension between 1.5 mm to 4 mm.
14. The method of claim 1 , wherein mechanically processing the stabilized three-dimensional structure to produce particles includes producing a group of particles comprising a longest dimension between 2 mm to 3.5 mm.
15. The method of claim 1 , wherein mechanically processing the stabilized three-dimensional structure to produce particles includes producing a group of particles comprising a longest dimension between 2 mm to 3 mm.
16. The method of claim 1 , wherein mechanically processing the stabilized three-dimensional structure to produce particles includes producing a group of particles having a micro-porous structure retained from the stabilized three-dimensional structure.
17. The method of claim 1 , further comprising hydrating the particles.
18. The method of claim 17 , wherein hydrating the particles includes combining the particles with a mass of an aqueous solution such that the particles and aqueous solution form a composition that is between 5% and 20% particles by weight.
19. The method of claim 18 , wherein hydrating the particles includes combining the particles with a mass of an aqueous solution such that the particles and aqueous solution form a composition that is between 5% and 12% particles by weight.