IP Library Granted Patent US 10,995,354
Granted Patent B2
US 10,995,354 · App. 16/165,372 · Granted May 4, 2021

Modification of RNA-related enzymes for enhanced production

Inventors: Bettina Strack-Logue (Lexington, MA); Frank DeRosa (Lexington, MA); Michael Heartlein (Lexington, MA)
Assignee: Translate Bio, Inc.
C12P19/34C07K14/47C12N9/1241C12N15/62C12Y207/0705C07K2319/35C07K2319/95
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Quick Facts
Patent No.
US 10,995,354
App. No.
16/165,372
Granted
May 4, 2021
Kind
B2
Abstract

The present invention provides, among other things, methods and compositions for large-scale production of capped mRNA using SUMO-Guanylyl Transferase fusion protein.

Claims (3)

1. A fusion protein, wherein the fusion protein comprises guanylyl transferase and a small ubiquitin-like molecule (SUMO) protein, wherein the fusion protein comprises SEQ ID NO: 8 and SEQ ID NO: 7.

2. The fusion protein of claim 1 , wherein the guanylyl transferase comprises a large subunit and a small subunit.

3. The fusion protein of claim 2 , wherein the SUMO protein is covalently linked and co-expressed with the large subunit.

Assignments (3)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Oct 19, 2018
From: STRACK-LOGUE, BETTINA; DEROSA, FRANK; HEARTLEIN, MICHAEL
To: SHIRE HUMAN GENETIC THERAPIES, INC.
Reel/Frame 047237/0001 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Oct 19, 2018
From: SHIRE HUMAN GENETIC THERAPIES, INC.
To: RANA THERAPEUTICS, INC.
Reel/Frame 047273/0835 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Oct 19, 2018
From: RANA THERAPEUTICS, INC.
To: TRANSLATE BIO, INC.
Reel/Frame 047872/0767 →
Continuity (3)
Continuation 15294249 · Oct 14, 2016
Provisional Application 62241350 · Oct 14, 2015
Related Publication 20190136283A1 · May 9, 2019
Cited By (1)
US 12,577,600