Methods for processing nucleic acid samples
The present disclosure provides methods and systems for amplifying and analyzing nucleic acid samples. The present disclosure provides methods for preparing cDNA and/or DNA molecules and cDNA and/or DNA libraries using modified reverse transcriptases.
1. A kit, comprising: an R2 reverse transcription enzyme comprising an amino acid sequence selected from the group consisting of SEQ ID NO: 49, SEQ ID NO: 50, SEQ ID NO: 51, SEQ ID NO: 53, SEQ ID NO: 54, SEQ ID NO: 55, SEQ ID NO: 56, SEQ ID NO: 57, SEQ ID NO: 58, SEQ ID NO: 59, SEQ ID NO: 60, SEQ ID NO: 61, SEQ ID NO: 62, SEQ ID NO: 63, SEQ ID NO: 64, SEQ ID NO: 65, SEQ ID NO: 66, and SEQ ID NO: 67.
2. The kit of claim 1 , wherein said R2 reverse transcription enzyme has a misincorporation error rate of at most about 1 base out of 100 bases.
3. The kit of claim 1 , wherein said R2 reverse transcription enzyme is configured to generate a plurality of complementary deoxyribonucleic acid molecules from one or more template ribonucleic acid molecules with a specific activity of from about 20,000 units/mg to about 140,000 units/mg.
4. The kit of claim 1 , wherein said R2 reverse transcription enzyme is capable of template jumping independent of sequence identity between said template ribonucleic acid molecule and an acceptor nucleic acid molecule.
5. The kit of claim 1 , wherein said R2 reverse transcription enzyme comprises SEQ ID NO:50.
6. The kit of claim 1 , wherein said R2 reverse transcription enzyme comprises SEQ ID NO:51.
7. The kit of claim 1 , wherein said R2 reverse transcription enzyme comprises SEQ ID NO:53.
8. The kit of claim 1 , wherein said R2 reverse transcription enzyme comprises SEQ ID NO:54.
9. The kit of claim 1 , wherein said R2 reverse transcription enzyme comprises SEQ ID NO:55.
10. The kit of claim 1 , wherein said R2 reverse transcription enzyme comprises SEQ ID NO:56.
11. The kit of claim 1 , wherein said R2 reverse transcription enzyme comprises SEQ ID NO:57.
12. The kit of claim 1 , wherein said R2 reverse transcription enzyme comprises SEQ ID NO:58.
13. The kit of claim 1 , wherein said R2 reverse transcription enzyme comprises SEQ ID NO:59.
14. The kit of claim 1 , wherein said R2 reverse transcription enzyme comprises SEQ ID NO:60.
15. The kit of claim 1 , wherein said R2 reverse transcription enzyme comprises SEQ ID NO:61.
16. The kit of claim 1 , wherein said R2 reverse transcription enzyme comprises SEQ ID NO:62.
17. The kit of claim 1 , wherein said R2 reverse transcription enzyme comprises SEQ ID NO:63.
18. The kit of claim 1 , wherein said R2 reverse transcription enzyme comprises SEQ ID NO:64.
19. The kit of claim 1 , wherein said R2 reverse transcription enzyme comprises SEQ ID NO:65.
20. The kit of claim 1 , wherein said R2 reverse transcription enzyme comprises SEQ ID NO:66.
21. The kit of claim 1 , wherein said R2 reverse transcription enzyme comprises SEQ ID NO:67.