Bioartificial liver
This document provides bioartificial liver (BAL) devices. Methods for making and using BAL devices also are provided.
1. A bioartificial liver device comprising a blood separation cartridge in fluid communication with a reservoir chamber, wherein said reservoir chamber comprises hepatocyte spheroids, a means for mixing, a mixing chamber, and a settling volume chamber, wherein said mixing chamber is located below said settling volume chamber and separated from said settling volume chamber by a funnel, wherein said mixing chamber is in fluid communication with said settling volume chamber via an open spout defined in said funnel, wherein said means for mixing is arranged in the mixing chamber and below said open spout, wherein said open spout is located from a bottom, inner surface of said mixing chamber at a distance configured to minimize net loss of said hepatocytes spheroids out of said mixing chamber to said settling volume chamber.
2. The bioartificial liver device of claim 1 , wherein said open spout is located less than 5 cm from said bottom, inner surface of said mixing chamber.
3. The bioartificial liver device of claim 1 , wherein said funnel comprises openings between 100 μm and 5 mm in diameter.
4. The bioartificial liver device of claim 1 , wherein said means for mixing is a magnetic stir bar within said mixing chamber.
5. The bioartificial liver device of claim 1 , wherein said mixing chamber comprises an inlet port.
6. The bioartificial liver device of claim 1 , wherein said settling volume chamber comprises an outlet port.
7. The bioartificial liver device of claim 1 , wherein said funnel comprises more than 25 openings.
8. The bioartificial liver device of claim 7 , wherein said openings are between 100 μm and 5 mm in diameter.
9. The bioartificial liver device of claim 1 , wherein said reservoir chamber comprises a sampling port.
10. The bioartificial liver device of claim 1 , wherein said reservoir chamber comprises temperature probe.