Detergent compositions comprising polypeptide variants

1. A detergent composition comprising a) a variant of a DNase parent, which compared to a DNase with SEQ ID NO: 1, comprises two or more substitutions selected from the group consisting of: T1I, T1L, T1V, S13Y, T22P, S25P, S27L, S39P, S42G, S42A, S42T, S57W, S57Y, S57F, S59V, S59I, S59L, V76L, V76I, Q109R, S116D, S116E, T127V, T127I, T127L, S144P, A147H, S167L, S167I, S167V, G175D and G175E, wherein the positions correspond to the positions of SEQ ID NO: 1 (numbering according to SEQ ID NO: 1), wherein the variant has a sequence identity to the polypeptide shown in SEQ ID NO: 1 or SEQ ID NO: 28 of at least 90% and wherein the variant has DNase activity; and b) from 0.01 to 99.9 wt % of an adjunct.

2. A detergent composition according to claim 1 wherein the variant comprises one or more substitution sets selected from the group consisting of: T1I+S13Y, T1I+T22P, T1I+S25P, T1I+S27L, T1I+S39P, T1I+S42G, T1I+S42A, T1I+S42T, T1I+S57W, T1I+S57Y, T1I+S57F, T1I+S59V, T1I+S591, T1I+S59L, T1I+V76L, T1I+V761, T1I+Q109R, T1I+S116D, T1I+S116E, T1I+T127V, T1I+T127I, T1I+T127L, T1I+S144P, T1I+A147H, T1I+S167L, T1I+S167I, T1I+S167V, T1I+G175D, T1I+G175E, T1L+S13Y, T1L+T22P, T1L+S25P, T1L+S27L, T1L+S39P, T1L+S42G, T1L+S42A, T1L+S42T, T1L+S57W, T1L+S57Y, T1L+S57F, T1L+S59V, T1L+S591, T1L+S59L, T1L+V76L, T1L+V761, T1L+Q109R, T1L+S116D, T1L+S116E, T1L+T127V, T1L+T1271, T1L+T127L, T1L+S144P, T1L+A147H, T1L+S167L, T1L+S1671, T1L+S167V, T1L+G175D, T1L+G175E, T1V+S13Y, T1V+T22P, T1V+S25P, T1V+S27L, T1V+S39P, T1V+S42G, T1V+S42A, T1V+S42T, T1V+S57W, T1V+S57Y, T1V+S57F, T1V+S59V, T1V+S59I, T1V+S59L, T1V+V76L, T1V+V76I, T1V+Q109R, T1V+S116D, T1V+S116E, T1V+T127V, T1V+T127I, T1V+T127L, T1V+S144P, T1V+A147H, T1V+S167L, T1V+S167I, T1V+S167V, T1V+G175D, T1V+G175E, S13Y+T22P, S13Y+S25P, S13Y+S27L, S13Y+S39P, S13Y+S42G, S13Y+S42A, S13Y+S42T, S13Y+S57W, S13Y+S57Y, S13Y+S57F, S13Y+S59V, S13Y+S59I, S13Y+S59L, S13Y+V76L, S13Y+V761, S13Y+Q109R, S13Y+S116D, S13Y+S116E, S13Y+T127V, S13Y+T127I, S13Y+T127L, S13Y+S144P, S13Y+A147H, S13Y+S167L, S13Y+S1671, S13Y+S167V, S13Y+G175D, S13Y+G175E, T22P+S25P, T22P+S27L, T22P+S39P, T22P+S42G, T22P+S42A, T22P+S42T, T22P+S57W, T22P+S57Y, T22P+S57F, T22P+S59V, T22P+S59I, T22P+S59L, T22P+V76L, T22P+V76I, T22P+Q109R, T22P+S116D, T22P+S116E, T22P+T127V, T22P+T127I, T22P+T127L, T22P+S144P, T22P+A147H, T22P+S167L, T22P+S1671, T22P+S167V, T22P+G175D, T22P+G175E, S25P+S27L, S25P+S39P, S25P+S42G, S25P+S42A, S25P+S42T, S25P+S57W, S25P+S57Y, S25P+S57F, S25P+S59V, S25P+S59I, S25P+S59L, S25P+V76L, S25P+V76I, S25P+Q109R, S25P+S116D, S25P+S116E, S25P+T127V, S25P+T127I, S25P+T127L, S25P+S144P, S25P+A147H, S25P+S167L, S25P+S167I, S25P+S167V, S25P+G175D, S25P+G175E, S27L+S39P, S27L+S42G, S27L+S42A, S27L+S42T, S27L+S57W, S27L+S57Y, S27L+S57F, S27L+S59V, S27L+S59I, S27L+S59L, S27L+V76L, S27L+V76I, S27L+Q109R, S27L+S116D, S27L+S116E, S27L+T127V, S27L+T127I, S27L+T127L, S27L+S144P, S27L+A147H, S27L+S167L, S27L+S167I, S27L+S167V, S27L+G175D, S27L+G175E, S39P+S42G, S39P+S42A, S39P+S42T, S39P+S57W, S39P+S57Y, S39P+S57F, S39P+S59V, S39P+S59I, S39P+S59L, S39P+V76L, S39P+V76I, S39P+Q109R, S39P+S116D, S39P+S116E, S39P+T127V, S39P+T127I, S39P+T127L, S39P+S144P, S39P+A147H, S39P+S167L, S39P+S167I, S39P+S167V, S39P+G175D, S39P+G175E, S42G+S57W, S42G+S57Y, S42G+S57F, S42G+S59V, S42G+S59I, S42G+S59L, S42G+V76L, S42G+V76I, S42G+Q109R, S42G+S116D, S42G+S116E, S42G+T127V, S42G+T1271, S42G+T127L, S42G+S144P, S42G+A147H, S42G+S167L, S42G+S167I, S42G+S167V, S42G+G175D, S42G+G175E, S42A+S57W, S42A+S57Y, S42A+S57F, S42A+S59V, S42A+S59I, S42A+S59L, S42A+V76L, S42A+V76I, S42A+Q109R, S42A+S116D, S42A+S116E, S42A+T127V, S42A+T1271, S42A+T127L, S42A+S144P, S42A+A147H, S42A+S167L, S42A+S1671, S42A+S167V, S42A+G175D, S42A+G175E, S42T+S57W, S42T+S57Y, S42T+S57F, S42T+S59V, S42T+S59I, S42T+S59L, S42T+V76L, S42T+V76I, S42T+Q109R, S42T+S116D, S42T+S116E, S42T+T127V, S42T+T127I, S42T+T127L, S42T+S144P, S42T+A147H, S42T+S167L, S42T+S167I, S42T+S167V, S42T+G175D, S42T+G175E, S57W+S59V, S57W+S59I, S57W+S59L, S57W+V76L, S57W+V76I, S57W+Q109R, S57W+S116D, S57W+S116E, S57W+T127V, S57W+T127I, S57W+T127L, S57W+S144P, S57W+A147H, S57W+S167L, S57W+S167I, S57W+S167V, S57W+G175D, S57W+G175E, S57Y+S59V, S57Y+S59I, S57Y+S59L, S57Y+V76L, S57Y+V76I, S57Y+Q109R, S57Y+S116D, S57Y+S116E, S57Y+T127V, S57Y+T1271, S57Y+T127L, S57Y+S144P, S57Y+A147H, S57Y+S167L, S57Y+S1671, S57Y+S167V, S57Y+G175D, S57Y+G175E, S57F+S59V, S57F+S59I, S57F+S59L, S57F+V76L, S57F+V76I, S57F+Q109R, S57F+S116D, S57F+S116E, S57F+T127V, S57F+T127I, S57F+T127L, S57F+S144P, S57F+A147H, S57F+S167L, S57F+S167I, S57F+S167V, S57F+G175D, S57F+G175E, S59V+V76L, S59V+V76I, S59V+Q109R, S59V+S116D, S59V+S116E, S59V+T127V, S59V+T127I, S59V+T127L, S59V+S144P, S59V+A147H, S59V+S167L, S59V+S167I, S59V+S167V, S59V+G175D, S59V+G175E, S59I+V76L, S59I+V761, S591+Q109R, S59I+S116D, S59I+S116E, S59I+T127V, S59I+T1271, S59I+T127L, S59I+S144P, S591+A147H, S59I+S167L, S59I+S1671, S59I+S167V, S59I+G175D, S59I+G175E, S59L+V76L, S59L+V761, S59L+Q109R, S59L+S116D, S59L+S116E, S59L+T127V, S59L+T127I, S59L+T127L, S59L+S144P, S59L+A147H, S59L+S167L, S59L+S1671, S59L+S167V, S59L+G175D, S59L+G175E, V76L+Q109R, V76L+S116D, V76L+S116E, V76L+T127V, V76L+T127I, V76L+T127L, V76L+S144P, V76L+A147H, V76L+S167L, V76L+S167I, V76L+S167V, V76L+G175D, V76L+G175E, V761+Q109R, V76I+S116D, V76I+S116E, V76I+T127V, V76I+T127I, V76I+T127L, V76I+S144P, V761+A147H, V76I+S167L, V76I+S1671, V76I+S167V, V76I+G175D, V76I+G175E, Q109R+S116D, Q109R+S116E, Q109R+T127V, Q109R+T127I, Q109R+T127L, Q109R+S144P, Q109R+A147H, Q109R+S167L, Q109R+S167I, Q109R+S167V, Q109R+G175D, Q109R+G175E, S116D+T127V, S116D+T127I, S116D+T127L, S116D+S144P, S116D+A147H, S116D+S167L, S116D+S167I, S116D+S167V, S116D+G175D, S116D+G175E, S116E+T127V, S116E+T127I, S116E+T127L, S116E+S144P, S116E+A147H, S116E+S167L, S116E+S167I, S116E+S167V, S116E+G175D, S116E+G175E, T127V+S144P, T127V+A147H, T127V+S167L, T127V+S1671, T127V+S167V, T127V+G175D, T127V+G175E, T127I+S144P, T127I+A147H, T127I+S167L, T127I+S1671, T127I+S167V, T127I+G175D, T127I+G175E, T127L+S144P, T127L+A147H, T127L+S167L, T127L+S1671, T127L+S167V, T127L+G175D, T127L+G175E, S144P+A147H, S144P+S167L, S144P+S1671, S144P+S167V, S144P+G175D, S144P+G175E, A147H+S167L, A147H+S1671, A147H+S167V, A147H+G175D, A147H+G175E, S167L+G175D, S167L+G175E, S167I+G175D, 51671-G175E, S167V+G175D and S167V+G175E.

3. A detergent composition according to claim 1 wherein in the DNase variant the two or more mutations are selected from the group consisting of T1I, S13Y, T22P, S25P, S27L, S39P, S42G, S57W, S59V, V76L, Q109R, S116D, T127V, S144P, A147H, S167L and G175D.

4. A detergent composition according to claim 3 wherein in the DNase variant the two or more mutations are selected from a set of substitutions selected from the group consisting of:

a. T1I+T22P+S57W+V76L+A147H+S167L,

b. T1I+T22P+S57W+V76L+A147H+G175D,

c. T1I+T22P+S57W+V76L+S167L+G175D,

d. T1I+T22P+S57W+A147H+S167L+G175D,

e. T1I+T22P+V76L+A147H+S167L+G175D,

f. T1I+S57W+V76L+A147H+S167L+G175D,

g. T22P+S57W+V76L+A147H+S167L+G175D, and

h. T1I+T22P+S57W+V76L+A147H+S167L+G175D.

5. A detergent composition according to claim 1 , wherein the variant comprises one or both motifs [D/M/L][S/T]GYSR[D/N] (SEQ ID NO: 25) and/or ASXNRSKG (SEQ ID NO: 26).

6. A detergent composition according to claim 1 wherein the DNase variant is selected from variants from the group comprising one of the following sets of substitutions, using numbering compared to SEQ ID NO: 1:

i. T1I+S13Y+T22P+S25P+S27L+S39P+S42G+S57W+S59V+V76L+S144P+A 147H+S167L+G175D;

ii. T1I+S13Y+T22P+S27L+S42G+S57W+S59V+V76L+Q109R+S116D+T127 V+S144P+A147H+S167L+G175D;

iii. T1I+S13Y+T22P+S25P+S27L+S39P+S42G+S57W+S59V+V76L+Q109R+S 116D+T127V+5144P+A147H+5167L+G175D;

iv. T1I+S13Y+T22P+S25P+S27L+S39P+S42G+S57W+S59V+V76L+T77Y+Q 109R+S116D+T127V+S144P+A147H+S167L+G175D;

v. T1I+T22P+D561+S57W+V76L+Q109R+S116D+A147H+G162S+S167L+G 175N+N178;

vi. T1I+S13Y+T22P+S27L+S39P+S42G+D561+S57W+S59V+V76L+Q109R+S 116D+T127V+S144P+A147H+S167L+G175D;

vii. T1I+T22P+S25P+S27L+S42G+D561+S57Y+S59V+V76L+T77Y+Q109R+S1 16D+T127V+S144P+A147H+Q166D+S167L+G175D+S181L;

viii. T1I+S13Y+T22P+S25P+S27L+S39P+S42G+D561+S57W+S59V+V76L+Q10 9R+S116D+T127V+S144P+A147H+S167L+G175D;

ix. T1I+S13Y+T22P+S25P+S27L+S39P+S42G+D561+S57W+S59V+V76L+Q1 09R+S116D+T127V+S144P+A147H+Q166D+S167L+G175D;

x. T1I+S13Y+T22P+S27R+S39P+S42G+D561+S57W+S59V+V76L+Q109R+S 116D+T127V+S144P+A147H+S167L+G175D;

xi. T1I+S13Y+T22P+S27L+S39P+S42G+D561+S57W+S59V+T65L+V76L+Q1 09R+S116D+T127V+S144P+A147H+S167L+G175D;

xii. T1I+S13Y+T22P+S27L+L33K+S39P+S42G+D561+S57W+S59V+T65V+V7 6L+Q109R+S116D+T127V+S144P+A147H+S167L+G175D;

xiii. T1I+S13Y+T22P+S25P+S27R+S39P+S42G+S57W+S59V+S66W+V76L+T 77Y+Q109R+S116D+T127V+S144P+A147H+S167L+G175D;

xiv. T1I+S13Y+T22P+S25P+S27L+L33K+S39P+S42G+S57W+S59V+T65V+V 76L+T77Y+Q109R+S116D+T127V+S144P+A147H+S167L+G175D;

xv. T1I+S13Y+T22P+S25P+S27L+L33K+S39P+S42G+S57W+S59V+S66Y+V7 6L+T77Y+Q109R+S116D+T127V+S144P+A147H+S167L+G175D;

xvi. T1I+S13Y+T22P+S25P+S27L+S39P+S42G+S57W+S59V+T65V+S66Y+V7 6L+T77Y+Q109R+S116D+T127V+S144P+A147H+S167L+G175D;

xvii. T1I+S13Y+T22P+S27L+S39P+S42G+D56L+S57W+S59V+T65V+V76L+Q 109R+S116D+T127V+S144P+A147H+G162D+S167L+G175D;

xviii. T1I+S13Y+T22P+S27R+S39P+S42G+D56L+S57W+S59V+T65V+V76L+Q 109R+S116D+T127V+S144P+A147H+S167L+G175D;

xix. T1I+S13Y+T22P+S27R+S39P+S42G+D56L+S57W+S59V+T65V+V76L+Q 109R+S116D+T127V+S144P+A147H+G162D+S167L+G175D;

xx. T1I+S13Y+T22P+S27K+S39P+S42G+D561+S57W+S59V+T65V+V76L+S1 06L+Q109R+S116D+T127V+S144P+A147H+S167L+G175D;

xxi. T1I+S13Y+T22P+S27K+S39P+S42G+D561+S57W+S59V+T65V+V76L+Q 109R+S116D+T127V+S130A+S144P+A147H+S167L+G175D;

xxii. T1I+S13Y+T22P+S27L+S39P+S42G+D56L+S57W+S59V+T65L+V76L+Q 109R+S116D+T127V+S130A+S144P+A147H+S167L+G175D; and

xxiii. T1I+S13Y+T22P+S27L+S39P+S42G+D561+S57W+S59V+T65V+V76L+Q1 09R+S116D+T127V+S144P+A147H+G162D+S167L+G175.

7. A detergent composition according to claim 1 , wherein the DNase variant has improved stability, measured as having an improvement factor Residual Activity Ratio (RAR)>1 or Half-life Improvement Factor (HIF)>1, compared to the parent DNase or compared to the DNase having the polypeptide shown in SEQ ID NO: 1 or compared to the DNase having the polypeptide shown in SEQ ID NO: 28.

8. A detergent composition according to claim 7 wherein the DNase variant has improved stability measured as Half-life Improvement Factor (HIF), of at least 1.2 compared to the parent DNase.

9. The DNase variant according to claim 7 wherein the DNase variant has improved residual activity ratio (RAR) being above 1 in at least one assay A, B or C.

10. A detergent composition according to claim 1 wherein the variant has at least 91% sequence identity to the polypeptide shown in SEQ ID NO: 1.

11. A detergent composition according to claim 1 wherein the variant further comprises one or more of the substitutions selected from the group consisting of: G4N, TSF, TSC, P6V, P6G, S7D, S7T, K8V, S9K, S9Q, S9V, S9L, S9F, S9P, S9R, A10D, A10M, A10I, A10Q, A10V, A10L, A10K, Q125, Q12V, Q12E, S13DQ14M, Q14R, N16S , A17C, A17V, A17E, A17T, T19K, T19L, T19S , T19I, T19V, K21E, K21M, G24Y, G28L, Y29W, S30K, 530D, S30H, 530T, D32Q, I38V, I38M, S39A, Q40V, V49R, L51I, K52I, K52H, A55S, D56I, D56L, D56T, Y58A, Y58T, N61D, P63A, T65L, T65I, T65V, T65R, T65K, S68V, S68I, S68W, S68Y, S68H, S68C, S68T, S68L, T77N, T77Y, T77W, T77R, F78I, F78H, F78Y, F78C, T79G, T79R, N80K, S82L, S82E, S82K, S82R, S82H, D83C, D83F, D83L, L92T, A93G, E94N, G99S, S101D, S101A, S102M, S102L, S102V, S102A, S102K, S102T, S102R, T104P, T104A, T105V, T105I, K107L, K107C, K107R, K107H, K107S, K107M, K107E, K107A, K107D, A112S , A125K, S126I, S126E, S126A, S126C, S130E, G132R, D135R, T138Q, W139R, R143E, R143K, G145V, G145E, G145D, G145A, G149S, K152H, K152R, S156C, S156G, S156K, S156R, S156T, S156A, T157S, Y159F, K160V, W161L, W161Y, G162Q, G162D, G162M, G162R, G162A, G162S, G162E, G162L, G162K, G162V, G162H, S164R, S164T, Q166D, S168V, S168E, S168D, S168L, K170S, K170L, K170F, K170R, T171D, T171E, T171A, T171C, A172G, A172S, L173T, L173A, L173V, Q174L, M176H, L177I, N178D, N178E, N178T, N178S, N178A, S179E, S181R, S181E, S181D, S181F, S181H, S181W, S181L, S181M, S181Y, S181Q, S181G, S181A, Y182M, Y182C, Y182K, Y182G, Y182A, Y182S, Y182V, Y182D, Y182Q, Y182F, Y182L, Y182N, Y182I, Y182E, Y182T and Y182W.

12. A detergent composition according to claim 1 wherein the variant is a variant of a parent DNase selected from the group of polypeptides:

a) a polypeptide having at least 90% sequence identity to the polypeptide shown in SEQ ID NO: 1,

b) a polypeptide having at least 90% sequence identity to the polypeptide shown in SEQ ID NO: 2,

c) a polypeptide having at least 90% sequence identity to the polypeptide shown in SEQ ID NO: 3,

d) a polypeptide having at least 90% sequence identity to the polypeptide shown in SEQ ID NO: 4,

e) a polypeptide having at least 90% sequence identity to the polypeptide shown in SEQ ID NO: 5,

f) a polypeptide having at least 90% sequence identity to the polypeptide shown in SEQ ID NO: 6,

g) a polypeptide having at least 90% sequence identity to the polypeptide shown in SEQ ID NO: 7,

h) a polypeptide having at least 90% sequence identity to the polypeptide shown in SEQ ID NO: 8,

i) a polypeptide having at least 90% sequence identity to the polypeptide shown in SEQ ID NO: 9,

j) a polypeptide having at least 90% sequence identity to the polypeptide shown in SEQ ID NO: 10,

k) a polypeptide having at least 90% sequence identity to the polypeptide shown in SEQ ID NO: 11,

l) a polypeptide having at least 90% sequence identity to the polypeptide shown in SEQ ID NO: 12,

m) a polypeptide having at least 90% sequence identity to the polypeptide shown in SEQ ID NO: 13,

n) a polypeptide having at least 90% sequence identity to the polypeptide shown in SEQ ID NO: 14,

o) a polypeptide having at least 90% sequence identity to the polypeptide shown in SEQ ID NO: 15,

p) a polypeptide having at least 90% sequence identity to the polypeptide shown in SEQ ID NO: 16,

q) a polypeptide having at least 90% sequence identity to the polypeptide shown in SEQ ID NO: 17,

r) a polypeptide having at least 90% sequence identity to the polypeptide shown in SEQ ID NO: 18,

s) a polypeptide having at least 90% sequence identity to the polypeptide shown in SEQ ID NO: 19,

t) a polypeptide having at least 90% sequence identity to the polypeptide shown in SEQ ID NO: 20,

u) a polypeptide having at least 90% sequence identity to the polypeptide shown in SEQ ID NO: 21,

v) a polypeptide having at least 90% sequence identity to the polypeptide shown in SEQ ID NO: 22,

w) a polypeptide having at least 90% sequence identity to the polypeptide shown in SEQ ID NO: 23, and

x) a polypeptide having at least 90% sequence identity to the polypeptide shown in SEQ ID NO: 24.

13. A detergent composition according to claim 1 wherein the adjunct comprises an additional enzyme selected from the group consisting of proteases, amylases, lipases, cutinases, cellulases, endoglucanases, xyloglucanases, pectinases, pectin lyases, xanthanases, peroxidaes, haloperoxygenases, catalases and mannanases, and mixtures thereof.

14. A detergent composition according to claim 1 wherein the adjunct comprises from 5 to 70 wt % surfactant.

15. A detergent composition according to claim 1 comprising (i) 2 to 50 wt % quaternary ammonium ester fabric softener compound selected from compounds of the following formula:

{R 2 (4-m) -N+—[X-Y-R 1 ] m }A-

wherein:

m is 1, 2 or 3 with proviso that the value of each m is identical;

each R 1 is independently hydrocarbyl, or branched hydrocarbyl group;

each R 2 is independently a methyl, ethyl, propyl, hydroxyethyl, 2-hydroxypropyl, 1-methyl-2-hydroxyethyl, poly(C 2-3 alkoxy), polyethoxy, benzyl;

each X is independently (CH 2 ) n , CH 2 —CH(CH 3 )— or CH—(CH 3 )—CH 2 — and

each n is independently 1, 2, 3 or 4;

each Y is independently —O—(O)C— or —C(O)—O—;

A- is independently selected from the group consisting of chloride, methyl sulfate, and ethyl sulfate; with the proviso that when Y is —O—(O)C—, the sum of carbons in each R 1 is from 13 to 21.

16. A method of washing comprising (i) contacting an article or surface with an aqueous wash liquor comprising a variant of a parent DNase, which compared to a DNase with SEQ ID NO: 1, comprises two or more substitutions selected from the group consisting of: T1I, T1L, T1V, 513Y, T22P, S25P, S27L, S39P, S42G, S42A, S42T, S57W, S57Y, S57F, S59V, S59I, S59L, V76L, V76I, Q109R, S116D, S116E, T127V, T1271, T127L, S144P, A147H, S167L, S1671, S167V, G175D and G175E, wherein the positions correspond to the positions of SEQ ID NO: 1 (numbering according to SEQ ID NO: 1), wherein the variant has a sequence identity to the polypeptide shown in SEQ ID NO: 1 or SEQ ID NO: 28 of at least 90% and wherein the variant has DNase activity; and an adjunct; and (ii) optionally rinsing and (iii) drying the surface.