Recombinant chimeric antigen receptor gene and use thereof
The invention provides a recombinant chimeric antigen receptor (CAR) gene, a vector containing the same, a CAR-T cell and a use thereof. The recombinant CAR gene comprises a nucleic acid sequence encoding an antigen-binding portion of a CD30 antibody, a transmembrane portion and a CD137 cytoplasmic functional region and a CD3zeta cytoplasmic functional region linked in any order; also provides a method of treating Hodgkin's lymphoma or anaplastic large cell lymphoma or other CD30-positive tumors using the CAR-T cells of the invention.
1. A product, which is a fusion polypeptide encoded by an isolated recombinant nucleic acid comprising nucleotide sequences encoding from 5′ to 3′an antigen-binding portion of a CD30 antibody-a transmembrane portion-a cytoplasmic functional region of CD137-a cytoplasmic functional region of CD3zeta,
wherein the recombinant nucleic acid comprises or consists of the sequence set forth in SEQ ID NO: 20 or 21, and
wherein the antigen binding portion of a CD30 antibody is an scFv.
2. A product, which is a fusion polypeptide encoded by an isolated recombinant nucleic acid comprising nucleotide sequences encoding from 5′ to 3′an antigen-binding portion of a CD30 antibody-a transmembrane portion-a cytoplasmic functional region of CD137-a cytoplasmic functional region of CD3zeta,
wherein the fusion polypeptide comprises or consists of the sequence set forth in SEQ ID NO: 19 or 22, and
wherein the antigen binding portion of a CD30 antibody is an scFv.