Biosensor array for the detection of analytes
Embodiments of the present disclosure relate generally devices for detecting analytes in a subject. More particularly, the present disclosure provides a biosensor array for detecting analytes in a subject. Embodiments of the present disclosure include a biosensor array comprising a plurality of sensor cells for detecting an analyte in a subject. In accordance with these embodiments, the plurality of sensor cells comprise at least one electrode, at least one antibody immobilized on a surface of the at least one electrode, and a biodegradable coating in contact with the at least one antibody.
1. An implantable biosensor for detecting insulin comprising:
an implantable biosensor array comprising a plurality of sensor cells comprising at least one working electrode, a counter electrode, and a reference electrode;
one or more insulin-specific antibodies immobilized on a surface of the at least one working electrode;
each sensor cell containing a biodegradable gelatin coating of varying thicknesses in direct contact with the one or more insulin-specific antibodies; and
where upon subcutaneous implantation in a subject, the various thicknesses of biodegradable gelatin coating degrade at different rates over a period of 1 week to 6 months thereby exposing the insulin-specific antibodies at varying time points.
2. The biosensor of claim 1 , wherein the biosensor array comprises at least 72 sensor cells.
3. The biosensor of claim 1 , wherein the biosensor array comprises 96 sensor cells.
4. The biosensor of claim 1 , wherein the biosensor array is about 0.1 mm-10 mm thick.
5. The biosensor of claim 1 , wherein the biosensor array is about 0.1 mm-5 mm thick.
6. A method for detecting insulin, the method comprising:
subcutaneously implanting the biosensor of claim 1 in a subject;
after the biodegradable gelatin coating degrades over the period of 1 week to 6 months exposing the insulin-specificantibody site, detecting insulin in the subject.