Techniques for enhancing the selectivity and efficacy of antimicrobial and anticancer polymer agents
The subject disclosure is directed to techniques for enhancing the selectivity and efficacy of therapeutic polymers against a broad spectrum of pathogens and cancer cell lines. According to an embodiment, a method is provided that comprises forming a therapeutic polymer based on polymerization of a plurality of therapeutic monomers, wherein the therapeutic polymer provides a therapeutic functionality. The method further comprises attaching biotin to the therapeutic polymer, resulting in a biotin-functionalized therapeutic polymer, wherein the biotin-functionalized therapeutic polymer provides greater therapeutic efficacy relative to the therapeutic polymer.
1. A method, comprising:
forming a therapeutic polymer based on a ring-opening polymerization of a plurality of cyclic carbonate monomers, wherein the therapeutic polymer provides at least one therapeutic functionality selected from the group consisting of antimicrobial functionality and anticancer functionality, and wherein the cyclic monomer comprises at least one protected functional group selected from the group consisting of a protected guanidinium functional group, a protected thiol functional group, and a protected carboxylic acid functional group; and
forming a biotin-functionalized therapeutic polymer by attaching biotin to an end of a backbone of the therapeutic polymer.
2. The method of claim 1 , wherein the protected functional group is the protected guanidinium functional group, and wherein the method further comprises:
forming a cationic guanidinium moiety attached to the backbone by deprotecting the protected guanidinium functional group.
3. The method of claim 2 , further comprising:
forming a coacervate complex by combining the biotin-functionalized therapeutic polymer with an anionic therapeutic polymer.
4. The method of claim 1 , wherein the wherein the protected functional group is the protected thiol functional group, and wherein the method further comprises:
forming a sulfur-hydrogen thiol by deprotecting the protected thiol functional group; and
forming a sulfonate moiety attached to the backbone by oxidizing the sulfur-hydrogen thiol.
5. The method of claim 4 , further comprising:
forming a coacervate complex by combining the biotin-functionalized therapeutic polymer with a cationic therapeutic polymer.
6. The method of claim 1 , wherein the protected functional group is the protected carboxylic acid functional group, and wherein the method further comprises:
forming an anionic carboxylic acid moiety attached to the backbone by deprotecting the protected carboxylic acid functional group.
7. The method of claim 6 , further comprising:
forming a coacervate complex by combining the biotin-functionalized therapeutic polymer with a cationic therapeutic polymer.
8. The method of claim 3 , wherein the the biotin-functionalized therapeutic polymer provides greater therapeutic efficacy relative to the therapeutic polymer for a treatment of cancer in cancer cell line BT 4-474 at a concentration rang of greater than or equal to 31 μg/mL and less than or equal to 250 μg/mL.