Cancer immunotherapy by disrupting PD-1/PD-L1 signaling
The disclosure provides a method for immunotherapy of a subject afflicted with cancer, comprises administering to the subject a composition comprising a therapeutically effective amount of an antibody that inhibits signaling from the PD-1/PD-L1 signaling pathway. This disclosure also provides a method for immunotherapy of a subject afflicted with cancer comprising selecting a subject that is a suitable candidate for immunotherapy based on an assessment that the proportion of cells in a test tissue sample from the subject that express PD-L1 on the cell surface exceeds a predetermined threshold level, and administering a therapeutically effective amount of an anti-PD-1 antibody to the selected subject. The invention additionally provides rabbit mAbs that bind specifically to a cell surface-expressed PD-L1 antigen in a FFPE tissue sample, and an automated IHC method for assessing cell surface expression in FFPE tissues using the provided anti-PD-L1 Abs.
1. A method of treating a tumor derived from a metastatic non-small cell lung cancer (NSCLC) in a human subject, comprising administering to the subject a therapeutically effective amount of an anti-PD-1 antibody; wherein the anti-PD-1 antibody is administered by intravenous infusion; wherein the tumor is refractory to a platinum-based chemotherapy; wherein at least 10% of tumor cells in the tumor exhibit membrane PD-L1 expression; and wherein the administering results in a reduction in the growth or size of the tumor that is refractory to a platinum-based chemotherapy.
2. The method of claim 1 , wherein the therapeutically effective amount of the anti-PD-1 antibody is administered once every 3 weeks.
3. The method of claim 1 , wherein the anti-PD-1 antibody is formulated in a pharmaceutical composition.
4. The method of claim 3 , wherein the pharmaceutical composition further comprises a pharmaceutically acceptable salt, an anti-oxidant, an aqueous carrier, a non-aqueous carrier, or any combination thereof.
5. The method of claim 4 , wherein the salt comprises a sodium salt.
6. The method of claim 4 , wherein the salt comprises sodium chloride.
7. The method of claim 1 , wherein the platinum-based chemotherapy comprises cisplatin, carboplatin, or both.
8. The method of claim 1 , wherein the membranous PD-L1 expression on the tumor cells is measured prior to administering the anti-PD-1 antibody.
9. The method of claim 8 , wherein the measuring comprises an immunohistochemistry.
10. The method of claim 9 , wherein the immunohistochemistry is performed using an antibody comprising:
(a) a heavy chain (HC) complementarity determining region (CDR) 1 comprising the amino acid sequence set forth in the HC-CDR1 of SEQ ID NO: 35;
(b) an HC-CDR2 comprising the amino acid sequence set forth in the HC-CDR2 of SEQ ID NO: 35;
(c) an HC-CDR3 comprising the amino acid sequence set forth in the HC-CDR3 of SEQ ID NO: 35;
(d) a light chain (LC) CDR1 comprising the amino acid sequence set forth in the LC-CDR1 of SEQ ID NO: 36;
(e) an LC-CDR2 comprising the amino acid sequence set forth in the LC-CDR2 of SEQ ID NO: 36; and
(f) an LC-CDR3 comprising the amino acid sequence set forth in the LC-CDR3 of SEQ ID NO: 36.
11. A method of treating a tumor derived from a metastatic NSCLC in a human subject, comprising administering to the subject a therapeutically effective amount of an anti-PD-1 antibody once every 3 weeks;
wherein the anti-PD-1 antibody is administered by intravenous infusion;
wherein the tumor is refractory to a platinum-based chemotherapy;
wherein at least 10% of tumor cells in the tumor exhibit membrane PD-L1 expression, as determined using an immunohistochemistry; and
wherein the administering results in a reduction in the growth or size of the tumor that is refractory to a platinum-based chemotherapy.
12. The method of claim 11 , wherein the anti-PD-1 antibody is formulated in a pharmaceutical composition.
13. The method of claim 12 , wherein the pharmaceutical composition further comprises a pharmaceutically acceptable salt, an anti-oxidant, an aqueous carrier, a non-aqueous carrier, or any combination thereof.
14. The method of claim 13 , wherein the salt comprises a sodium salt.
15. The method of claim 13 , wherein the salt comprises sodium chloride.
16. The method of claim 11 , wherein the platinum-based chemotherapy comprises cisplatin, carboplatin, or both.
17. The method of claim 11 , wherein the immunohistochemistry is performed using an antibody comprising:
(a) a heavy chain (HC) complementarity determining region (CDR) 1 comprising the amino acid sequence set forth in the HC-CDR1 of SEQ ID NO: 35;
(b) an HC-CDR2 comprising the amino acid sequence set forth in the HC-CDR2 of SEQ ID NO: 35;
(c) an HC-CDR3 comprising the amino acid sequence set forth in the HC-CDR3 of SEQ ID NO: 35;
(d) a light chain (LC) CDR1 comprising the amino acid sequence set forth in the LC-CDR1 of SEQ ID NO: 36;
(e) an LC-CDR2 comprising the amino acid sequence set forth in the LC-CDR2 of SEQ ID NO: 36; and
(f) an LC-CDR3 comprising the amino acid sequence set forth in the LC-CDR3 of SEQ ID NO: 36.