Fast two-photon imaging by diffracted swept-laser excitation
An apparatus and methods for high-speed non-linear spectrally encoded multi-photon imaging that are particularly suited for use in two photon fluorescence and fluorescence lifetime imaging. The system is capable of optical image compression and scale invariant digital zoom. A wavelength agile laser with digitally synthesized electro-optic modulation in a master oscillator-power amplifier configuration is combined with spectral encoding to eliminate the speed limitations of inertial scanning. The technique for fast two photon fluorescent imaging with simultaneous lifetime imaging independently detects the location, amplitude and lifetime of fluorescent emission by synthesizing a sequential excitation beam via digital electro-optic modulation of a quasi-CW swept source followed by time encoded detection. For fluorescent imaging, spectral and temporal mappings are employed separately, with quasi-CW spectral encoding used for pumping and time encoding for constructing the image at fluorescence wavelength.
1. A laser scanning apparatus, comprising:
(a) a wavelength agile laser multi-color light source;
(b) a modulator configured for modulating said multi-color light source into a dynamic time pattern; and
(c) a diffraction grating configured for diffracting said dynamic time pattern of wavelength agile light onto a sample;
(d) wherein different wavelengths of light are preferentially diffracted at different angles.
2. The apparatus of claim 1 , wherein the wavelength agile laser light source is a wavelength swept laser.
3. The apparatus of claim 1 , said modulator further comprising:
an optical amplifier for amplifying the modulator output to instantaneous powers higher than an average power.
4. The apparatus of claim 1 , wherein said modulator comprises stimulated Raman scattering (SRS) providing both pulse modulation and amplification.
5. The apparatus of claim 1 , wherein the dynamic time pattern of the modulator encodes a diffraction pattern imposed on the sample.
6. The apparatus of claim 1 , wherein the diffraction grating comprises a spatially adjustable grating.
7. The apparatus of claim 1 , further comprising:
an objective lens for focusing diffracted beams on to the sample.
8. The apparatus of claim 1 , further comprising:
a detector configured to detect interactions of the dynamic time pattern with the sample; and
an imager.
9. The apparatus of claim 1 , further comprising:
a controller configured to control laser, modulator, diffraction grating and detector functions.
10. The apparatus of claim 9 , wherein an illumination pattern applied to the sample can be dynamically formed by control of one or more of the wavelength outputs of the agile laser, the modulation dynamic time pattern, and an adjustable diffraction grating.
11. The apparatus of claim 9 , further comprising:
an electro-optical modulator configured for time modulation; and
a time-dependent bias voltage applied to the electro-optical modulator by the controller to match a wavelength dependent bias voltage to the instantaneous color output of the wavelength agile laser.
12. The apparatus of claim 1 , wherein the detector is selected from the group of detectors consisting of an avalanche photodetector, a photomultiplier tube, a hybrid photodetector, a multichannel plate, charged coupled detector, a CMOS detector, an arrayed detector, a gated detector, and an image intensifier.
13. A laser scanning apparatus, comprising:
(a) a wavelength swept laser configured for generating a broadband light source;
(b) a pulse modulator configured for modulating said broadband light source into short pulses; and
(c) a diffraction grating configured for diffracting said wavelength swept short pulses into a spectral brush directed onto a line or a two-dimensional array on a sample; and
(d) a detector configured to detect interactions of the spectral brush with the sample;
(e) wherein each of said short pulses are diffracted at a different angle in response to their differing wavelength inducing a pixel wise interaction with the sample.
14. The apparatus of claim 13 , further comprising:
an optical amplifier for amplifying the short pulses.
15. The apparatus of claim 13 , further comprising:
an adjustable beam scanner configured to direct beams from the diffraction grating to the sample.
16. The apparatus of claim 15 , further comprising:
an objective lens for focusing diffracted beams from the beam scanner on to the sample.
17. The apparatus of claim 16 , wherein individual pulse length, repetition rate, pulse pattern, individual pulse height and individual pulse form of the broadband light source are controlled by the controller.
18. The apparatus of claim 15 , further comprising:
a controller configured to control laser, modulator, diffractive element, beam scanner and detector functions.
19. The apparatus of claim 18 , wherein the wavelength swept laser is controlled with an electronic waveform from the controller to produce a linear wavelength sweep.
20. The apparatus of claim 18 , wherein the wavelength swept laser is controlled with an electronic waveform from the controller to produce a k-space linear frequency sweep.
21. The apparatus of claim 18 , wherein detection by the detector is synchronized with the pulse modulation by the controller to only detect signals generated synchronously with the applied pulses thereby suppressing any background signals.
22. The apparatus of claim 18 , wherein the modulator is operated by the controller with both a pulse pattern and a sweep pattern to improve modulation depth of the pulses.
23. The apparatus of claim 18 , wherein a digitally sculpted waveform is applied to the pulse modulation of the modulator by the controller to produce non-uniform sampling of the sample.
24. The apparatus of claim 13 , wherein a non-linear interaction comprising the simultaneous absorption of two or more photons is induced at the sample.
25. The apparatus of claim 13 , wherein said interaction detected by the detector is selected from the group consisting of absorption, non-linear absorption, reflection, scattering, ionization, plasma formation, polymerization, lithography, ablation, spectroscopy, and laser induced breakdown spectroscopy.
26. The apparatus of claim 13 , further comprising:
a plate with at least one fluidic channel with a fluidic flow where sample objects pass the optical beam in flow;
wherein fluorescence lifetime imaging is performed of the objects in flow.
27. The apparatus of claim 13 , further comprising an imager operably coupled to the detector.