Plants with modified deoxyhypusine synthase genes
The invention relates to methods of producing a plant with delayed senescence comprising inducing at least one nucleotide deletion, insertion or substitution into at least one copy of a gene encoding deoxyhypusine synthase (DHS) in the plant, wherein the nucleotide deletion, insertion or substitution decreases the activity of DHS encoded by the gene in the plant. The invention also relates to plants produced by the methods and progeny thereof.
1. A method of producing a plant with delayed senescence relative to a wild-type control plant, the method comprising inducing at least one nucleotide substitution into at least one copy of a gene encoding deoxyhypusine synthase (DHS) in the plant in a codon for at least one amino acid selected from the group consisting of K144, K292, D318, E328, A329, V330, S331, W332, K334, K343 of SEQ ID NO: 2, or a corresponding amino acid in another plant species, wherein the nucleotide substitution decreases the activity of DHS encoded by the gene in the plant relative to the activity of DHS in the wild-type control plant so as to delay senescence.
2. The method of claim 1 , wherein the delayed senescence
a) increases seed yield in the plant relative to a wild-type control plant,
b) increases leaf and root biomass relative to a wild-type control plant,
c) enhances plant survival during drought or nutrient stress relative to a wild-type control plant,
d) increases disease resistance of the plant relative to a wild-type control plant, and/or
e) increases the period of time during which leaves, stems, seeds and fruit of the plant may be stored and remain suitable for use relative to a wild-type control plant.
3. The method of claim 1 , wherein the plant is haploid, diploid, tetraploid, or polyploid.
4. The method of claim 1 comprising inducing at least one nucleotide substitution into at least two copies of a gene encoding DHS in the plant.
5. The method of claim 1 , wherein the senescence is age-related senescence.
6. The method of claim 1 , wherein the senescence is environmental stress-induced senescence.
7. A plant produced by the method of claim 1 .
8. Progeny of the plant according to claim 7 , wherein the progeny comprises the nucleotide substitution.
9. The method of claim 1 , wherein the senescence is plant pathogen-induced senescence.
10. The method of claim 1 , wherein the plant is selected from the group consisting of Triticum aestivum, Brassica napus, Gossypium hirsutum, Cicer arietinum, Glycine max, Phaseolus vulgar, Populus deltoides, Beta vulgaris, Oryza sativa, Sorghum bicolor, Zea mays, Brassica rapa, Arachis hypogaea, Vitis vinifera, Vitis labrusca, Theobroma cacao, Camellia sinensis, Lactuca sativa, Manihot esculenta, Phalaenopsis equestris, Coffea canephora, Camelina sativa, Musa acuminate, Medicago sativa, Rosa chinensis, Solanum lycopersicum , and Solanum tuberosum.
11. A method of producing a plant with delayed senescence relative to a wild-type control plant, the method comprising inducing at least one nucleotide deletion or insertion into at least one copy of a gene encoding deoxyhypusine synthase (DHS) in the plant in a codon for at least one amino acid selected from the group consisting of K144, K292, D318, E328, A329, V330, S331, W332, K334, K343 of SEQ ID NO: 2, or a corresponding amino acid in another plant species, wherein the nucleotide deletion or insertion decreases the activity of DHS encoded by the gene in the plant relative to the activity of DHS in the wild-type control plant so as to delay senescence, and the plant contains at least one copy of the gene encoding DHS without the deletion or insertion.
12. The method of claim 11 , wherein the delayed senescence
a) increases seed yield in the plant relative to a wild-type control plant,
b) increases leaf and root biomass relative to a wild-type control plant,
c) enhances plant survival during drought or nutrient stress relative to a wild-type control plant,
d) increases disease resistance of the plant relative to a wild-type control plant, and/or
e) increases the period of time during which leaves, stems, seeds and fruit of the plant may be stored and remain suitable for use relative to a wild-type control plant.
13. The method of claim 11 , wherein the plant is haploid, diploid, tetraploid, or polyploid.
14. The method of claim 11 , wherein the senescence is age-related senescence.
15. The method of claim 11 , wherein the senescence is environmental stress-induced senescence.
16. The method of claim 11 , wherein the senescence is plant pathogen-induced senescence.
17. A plant produced by the method of claim 11 .
18. Progeny of the plant according to claim 17 , wherein the progeny comprises the nucleotide deletion or insertion.
19. The method of claim 11 , wherein the plant is selected from the group consisting of Triticum aestivum, Brassica napus, Gossypium hirsutum, Cicer arietinum, Glycine max, Phaseolus vulgar, Populus deltoides, Beta vulgaris, Oryza sativa, Sorghum bicolor, Zea mays, Brassica rapa, Arachis hypogaea, Vitis vinifera, Vitis labrusca, Theobroma cacao, Camellia sinensis, Lactuca sativa, Manihot esculenta, Phalaenopsis equestris, Coffea canephora, Camelina sativa, Musa acuminate, Medicago sativa, Rosa chinensis, Solanum lycopersicum , and Solanum tuberosum.