Biofunctional materials
Provided are methods and compositions for self-cleaning that include a digestive protein capable of decomposing stain forming molecules, a substrate applied to a solid surface, and a linker moiety bound to an outer surface of said substrate and an active group of said digestive protein, said linker moiety between said protein and said substrate and covalently linking said protein to a surface of said substrate by an amide bond, the linker moiety between a free amine of said protein and said outer surface of said substrate wherein the digestive protein forms a layer on a surface of said substrate such that the digestive protein is surface exposed for reaction with a stain.
1. A composition for removing stains from a solid surface comprising:
a digestive protein capable of decomposing stain forming molecules;
a substrate applied to the solid surface; and
a linker moiety bound to an outer surface of said substrate and an active group of said digestive protein, said linker moiety between said protein and said substrate and covalently linking said protein to a surface of said substrate by an amide bond, the linker moiety between a free amine of said protein and said outer surface of said substrate;
said digestive protein forming a layer on a surface of said substrate such that the digestive protein is surface exposed for reaction with a stain.
2. The composition according to claim 1 , wherein the digestive protein comprises a lysozyme, protease, lipase, cellulase, glycosidase, or amylase.
3. The composition according to claim 1 wherein said free amine is in a lysine, arginine, asparagine, glutamine, or an N-terminal end.
4. The composition according to claim 1 , wherein the digestive protein comprises a lipase.
5. The composition according to claim 1 , wherein said substrate comprises paint or polymers.