Detection of vitamins A and E by tandem mass spectrometry
View Patent ↗Methods are described for measuring the amount of one or more of vitamin A, α-tocopherol, and the combination of β-tocopherol and γ-tocopherol in a sample. More specifically, mass spectrometric methods are described for detecting and quantifying one or more of vitamin A, α-tocopherol, and the combination of β-tocopherol and γ-tocopherol in a sample.
1. A method for determining an amount of vitamin A in a sample by mass spectrometry, said method comprising:
a. adding one or more isotopically labeled internal standards to the sample;
b. subjecting the sample to turbulent flow liquid chromatography (TFLC) to provide an extracted sample;
c. subjecting the extracted sample to high pressure liquid chromatography (HPLC) to provide a purified sample;
d. ionizing vitamin A from the purified sample to produce one or more vitamin A ions detectable by mass spectrometry; and
e. determining an amount of one or more of said Vitamin A ions by mass spectrometry, wherein the one or more said vitamin A ions comprise a fragment ion having a mass to charge ratio of about 105.00±0.80;
wherein the amount of the one or more ions determined in step (e) is related to the amount of vitamin A in the sample.
2. The method of claim 1 , wherein the mass spectrometry is tandem mass spectrometry.
3. The method of claim 1 , wherein said HPLC and said ionization are conducted with on-line processing.
4. The method of claim 1 , wherein said ionization comprises ionization with an atmospheric chemical ionization (APCI) source.
5. The method of claim 1 , wherein the sample comprises a biological sample.
6. The method of claim 5 , wherein the biological sample is from a human patient suspected of having a deficiency or an excess of vitamin A.
7. The method of claim 1 , wherein the sample comprises serum or plasma.
8. The method of claim 1 , wherein the mass spectrometry is conducted by multiple reaction monitoring, precursor ion scanning, or product ion scanning.
9. The method of claim 1 , the method further comprising determining an amount of one or more additional analytes simultaneously with the determination of the amount of vitamin A.
10. The method of claim 9 , wherein the one or more additional analytes comprises one or more of α-tocopherol, β-tocopherol, and γ-tocopherol.
11. The method of claim 10 , wherein the one or more isotopically labeled internal standard is d 9 -α-tocopherol.
12. The method of claim 9 , wherein the one or more additional analytes comprises α-tocopherol, and determining the amount of α-tocopherol comprises fragmenting an α-tocopherol precursor ion having a mass to charge ratio of about 430.47±0.80 into one or more fragment ions comprising a fragment ion having a mass to charge ratio of about 165.03±0.80.
13. The method of claim 9 , wherein the one or more additional analytes comprises combined β-tocopherol and γ-tocopherol, and determining the amount of combined β-tocopherol and γ-tocopherol comprises fragmenting β-tocopherol and γ-tocopherol precursor ions having a mass to charge ratio of about 416.35±0.80 into one or more fragment ions comprising a fragment ion having a mass to charge ratio of about 151.00±0.80.
14. The method of claim 9 , wherein the one or more additional analytes comprises α-tocopherol and combined β-tocopherol and γ-tocopherol.
15. The method of claim 1 , wherein the one or more isotopically labeled internal standard is d 5 -vitamin A.