Method for improved production of fermentable sugars from lignocellulosic biomass using additive and enzyme
The present invention relates to an additive or additive composition for improved production of fermentable sugars from lignocellulosic biomass during enzymatic hydrolysis. More particularly, the present invention discloses petrochemical waste as an additive or an additive composition containing one or more ethylene glycol oligomers and one or more metal ions which are useful for improved production of fermentable sugars from lignocellulosic biomass during enzymatic hydrolysis.
1. A method for production of fermentable sugars from lignocellulosic biomass, comprising:
a) feeding a grounded lignocellulosic biomass feedstock into a pretreatment reactor system for dilute acid impregnation at room temperature or at a temperature ranging from 50-100° C. for a period 5-60 minutes to render it acid-soaked;
b) removing 40% 70% acid from said acid-soaked biomass by pressing in a hydraulic press to form a pressed biomass;
c) feeding said pressed biomass to a plug mill using feed hopper with feed rate of 8-12 kg/hour for conversion into solid plug(s) of the biomass;
d) treating said solid plug(s) with dilute acid in an amount of 0.02 to 3% w/w of the biomass having pH 1-3 at 120-200° C. for a residence time in the range of 8-12 minutes for conversion into biomass slurry;
e) centrifuging said biomass slurry for separating solids and liquids, followed by washing solids to a pH of 7.0; and
f) hydrolyzing said solids with an additive and enzyme for 40-50 hours in an incubator shaker at 45-55° C. having speed of 180-220 rpm to obtain fermentable sugars.
2. The method as claimed in claim 1 , wherein the dilute acid used in the pretreatment step is selected from the group consisting of sulphuric acid, hydrochloric acid, acetic acid and phosphoric acid.
3. The method as claimed in claim 1 , wherein the additive is a petrochemical waste.
4. The method as claimed in claim 3 , wherein the petrochemical waste comprises one or more ethylene glycol oligomer(s) and one or more metal(s).
5. The method as claimed in claim 4 , wherein the ethylene glycol oligomer is selected from the group consisting of monoethylene glycol (MEG) present in an amount ranging from 0.0-0.5%, diethylene glycol (DEG) present in an amount ranging from 0.5-30.0%, triethylene glycol (TEG) present in an amount ranging from 40.0-50.0%, and tetraethylene glycol (TTEG) present in an amount ranging from 25-50%.
6. The method as claimed in claim 4 , wherein the metal is selected from the group consisting of iron (Fe) in an amount of 0.58%, sodium (Na) in an amount of 0.02%, Chromium (Cr) in an amount of 0.015%, Boron (B) in an amount of 0.012% and manganese (Mn) in an amount of 0.0078%.
7. The method as claimed in claim 4 , wherein the petrochemical waste is for production of fermentable sugars from lignocellulosic biomass.